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David Wu

TitleInstructor
InstitutionUniversity of Chicago
DepartmentMedicine-Pulmonary/Critical Care
AddressChicago IL 60637
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    Collapse Overview 
    Collapse overview
    Our lab is interested in how mechanotransduction affects endothelial cell phenotype. We recently discovered that oscillatory shear stress affects endothelial cell metabolism by turning on HIF-1alpha which turns up glycolytic pathways and suppresses mitochondrial function. This metabolic switch, induced by mechanical forces, is necessary for promoting endothelial inflammation. Currently, we are using novel FRET probes to investigate how modulating the actin cytoskeleton affects metabolism at the single cell level.


    Collapse Biography 
    Collapse education and training
    University of ChicagoBS2001Biological Chemistry
    California Institute of TechnologyPhD2010Bioengineering
    University of California, Los AngelesMD2012Medicine
    University of Chicago2014Internal Medicine Residency
    University of Chicago2018Pulmonary and Critical Care Fellowship
    University of Chicago2018Postdoctoral Fellowship, Mutlu and Fang labs

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Krause MD, Huang RT, Wu D, Shentu TP, Harrison DL, Whalen MB, Stolze LK, Di Rienzo A, Moskowitz IP, Civelek M, Romanoski CE, Fang Y. Genetic variant at coronary artery disease and ischemic stroke locus 1p32.2 regulates endothelial responses to hemodynamics. Proc Natl Acad Sci U S A. 2018 11 27; 115(48):E11349-E11358. PMID: 30429326.
      View in: PubMed
    2. Wu D, Woods PS, Duong HT, Mutlu GM. Role of Cellular Metabolism in Pulmonary Diseases. Am J Respir Cell Mol Biol. 2018 07; 59(1):127-129. PMID: 29634283.
      View in: PubMed
    3. Hamanaka RB, Nigdelioglu R, Meliton AY, Tian Y, Witt LJ, O'Leary E, Sun KA, Woods PS, Wu D, Ansbro B, Ard S, Rohde JM, Dulin NO, Guzy RD, Mutlu GM. Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis. Am J Respir Cell Mol Biol. 2018 05; 58(5):585-593. PMID: 29019702.
      View in: PubMed
    4. Yi-Ju Chen, David Wu, William Gelbart, Charles M. Knobler, Rob Phillips, and Willem K. Kegel. Two-stage dynamics of in vivo bacteriophage genome ejection. Physical Review X. 2018; 8:021029.
    5. Wu D, Hamanaka RB, Fang Y, Mutlu GM. Letter by Wu et al Regarding Article, "Mechanical Activation of Hypoxia-Inducible Factor 1a Drives Endothelial Dysfunction at Atheroprone Sites". Arterioscler Thromb Vasc Biol. 2017 12; 37(12):e197-e198. PMID: 29162600.
      View in: PubMed
    6. Wu D, Huang RT, Hamanaka RB, Krause M, Oh MJ, Kuo CH, Nigdelioglu R, Meliton AY, Witt L, Dai G, Civelek M, Prabhakar NR, Fang Y, Mutlu GM. HIF-1a is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. Elife. 2017 05 30; 6. PMID: 28556776.
      View in: PubMed
    7. Huang RT, Wu D, Meliton A, Oh MJ, Krause M, Lloyd JA, Nigdelioglu R, Hamanaka RB, Jain MK, Birukova A, Kress JP, Birukov KG, Mutlu GM, Fang Y. Experimental Lung Injury Reduces Krüppel-like Factor 2 to Increase Endothelial Permeability via Regulation of RAPGEF3-Rac1 Signaling. Am J Respir Crit Care Med. 2017 03 01; 195(5):639-651. PMID: 27855271.
      View in: PubMed
    8. Nigdelioglu R, Hamanaka RB, Meliton AY, O'Leary E, Witt LJ, Cho T, Sun K, Bonham C, Wu D, Woods PS, Husain AN, Wolfgeher D, Dulin NO, Chandel NS, Mutlu GM. Transforming Growth Factor (TGF)-ß Promotes de Novo Serine Synthesis for Collagen Production. J Biol Chem. 2016 12 30; 291(53):27239-27251. PMID: 27836973.
      View in: PubMed
    9. Anton H, Harlepp S, Ramspacher C, Wu D, Monduc F, Bhat S, Liebling M, Paoletti C, Charvin G, Freund JB, Vermot J. Pulse propagation by a capacitive mechanism drives embryonic blood flow. Development. 2013 Nov; 140(21):4426-34. PMID: 24089470.
      View in: PubMed
    10. Van Valen D, Wu D, Chen YJ, Tuson H, Wiggins P, Phillips R. A single-molecule Hershey-Chase experiment. Curr Biol. 2012 Jul 24; 22(14):1339-43. PMID: 22727695.
      View in: PubMed
    11. Qiu W, Derr ND, Goodman BS, Villa E, Wu D, Shih W, Reck-Peterson SL. Dynein achieves processive motion using both stochastic and coordinated stepping. Nat Struct Mol Biol. 2012 Jan 08; 19(2):193-200. PMID: 22231401.
      View in: PubMed
    12. Trinh le A, Hochgreb T, Graham M, Wu D, Ruf-Zamojski F, Jayasena CS, Saxena A, Hawk R, Gonzalez-Serricchio A, Dixson A, Chow E, Gonzales C, Leung HY, Solomon I, Bronner-Fraser M, Megason SG, Fraser SE. A versatile gene trap to visualize and interrogate the function of the vertebrate proteome. Genes Dev. 2011 Nov 01; 25(21):2306-20. PMID: 22056673.
      View in: PubMed
    13. Wu D, Freund JB, Fraser SE, Vermot J. Mechanistic basis of otolith formation during teleost inner ear development. Dev Cell. 2011 Feb 15; 20(2):271-8. PMID: 21316594.
      View in: PubMed
    14. Wu D, Van Valen D, Hu Q, Phillips R. Ion-dependent dynamics of DNA ejections for bacteriophage lambda. Biophys J. 2010 Aug 09; 99(4):1101-9. PMID: 20712993.
      View in: PubMed
    15. Pantazis P, Maloney J, Wu D, Fraser SE. Second harmonic generating (SHG) nanoprobes for in vivo imaging. Proc Natl Acad Sci U S A. 2010 Aug 17; 107(33):14535-40. PMID: 20668245.
      View in: PubMed
    16. Vermot J, Forouhar AS, Liebling M, Wu D, Plummer D, Gharib M, Fraser SE. Reversing blood flows act through klf2a to ensure normal valvulogenesis in the developing heart. PLoS Biol. 2009 Nov; 7(11):e1000246. PMID: 19924233.
      View in: PubMed
    17. Wu D, Ghosh K, Inamdar M, Lee HJ, Fraser S, Dill K, Phillips R. Trajectory approach to two-state kinetics of single particles on sculpted energy landscapes. Phys Rev Lett. 2009 Jul 31; 103(5):050603. PMID: 19792475.
      View in: PubMed
    18. Colantonio JR, Vermot J, Wu D, Langenbacher AD, Fraser S, Chen JN, Hill KL. The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature. 2009 Jan 08; 457(7226):205-9. PMID: 19043402.
      View in: PubMed
    19. Kulkarni RP, Wu DD, Davis ME, Fraser SE. Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy. Proc Natl Acad Sci U S A. 2005 May 24; 102(21):7523-8. PMID: 15897455.
      View in: PubMed
    20. Xu S, Wu D, Arnsdorf M, Johnson R, Getz GS, Cabana VG. Chemical colloids versus biological colloids: a comparative study for the elucidation of the mechanism of protein fiber formation. Biochemistry. 2005 Apr 12; 44(14):5381-9. PMID: 15807531.
      View in: PubMed
    21. Qu X, Wu D, Mets L, Scherer NF. Nanometer-localized multiple single-molecule fluorescence microscopy. Proc Natl Acad Sci U S A. 2004 Aug 03; 101(31):11298-303. PMID: 15277661.
      View in: PubMed
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