AkiraImamotoAkira Imamoto41.78927490000000-87.601250000000001744Imamoto, AkiraDiGeorge SyndromeVelocardiofacial SyndromeCardiovascular DiseasesPharyngeal ArchesSignal Transduction PathwaysNetwork, GeneWe are interested in elucidating mechanisms by which a mutation or combination of mutations affect broader network within and between cells during development. Our current model is DiGeorge/velocardiofacial/del22q11 syndrome (OMIM: 188400 and 192430), a contiguous gene syndrome in approximately 1 in 4000 live births. The common manifestations of this syndrome include cardiovascular defects, aplasia or hypoplasia of the thymus and parathyroid glands, and craniofacial anomalies. Urogenital defects, learning disabilities, and other psychiatric disorders are also common. Two 22q11 genes, TBX1 and CRKL, have been implicated in this syndrome. While they show a strong dosage-sensitive interaction in mouse models, they encode different classes of proteins. Tbx1 is a T-box transcription factor, while CRKL is an adapter protein presumably down stream of tyrosine kinases. To date, few direct links have been known between the signaling pathways in which they are involved. Using multitudes of techniques, we aim to find missing pieces to establish their functional links.Associate Professorprns:coAuthorOfcoauthor ofprns:emailEncryptedemail addressprns:endDateend dateprns:fullNamefull nameprns:grantAwardedBygrant awarded byprns:hasAuthorListauthor listprns:hasNetworkhas networkprns:hasPublicationVenuepublished inprns:informationResourceReferenceinformation resource referenceprns:isPrimaryPositionis primary positionprns:latitudelatitudeprns:longitudelongitudeprns:mainImagephotoprns:maxWeightmaximum weightprns:medlineTAjournal title abbreviationprns:meshDescriptorUIMeSH DescriptorUIprns:meshSemanticGroupNameMeSH semantic group nameprns:minWeightminimum weightprns:numberOfAuthorsnumber of authorsprns:numberOfConnectionsnumber of connectionsprns:numberOfPublicationsnumber of publicationsprns:personIdPerson IDprns:personInPrimaryPositionperson in primary positionprns:physicalNeighborOfphysical neighborprns:positionInDepartmentposition in departmentprns:predicateNodepredicate nodeprns:principalInvestigatorNameprincipal investigator nameprns:publicationDatepublication dateprns:similarTosimilar toprns:sortOrdersort orderprns:startDatestart dateprns:trainingAtOrganizationeducational organizationprns:trainingLocationtraining locationprns:uniquenessWeightuniqueness weightprns:yearyearAcademic ArticleArticleDocumentbibo:pmidPubMed IdentifierAddressvivo:addressCitycityvivo:addressPostalCodepostal codevivo:addressStatestate or provinceAgreementvivo:authorInAuthorshipselected publicationsvivo:authorRankauthor rank in publicationAuthorshipvivo:degreeEarneddegree earnedDepartmentvivo:educationalTrainingeducation and trainingEducational Trainingvivo:freetextKeywordkeywordsGrantvivo:hasResearchArearesearch areasvivo:hasResearcherRoleresearch activitiesvivo:hrJobTitleHR job titleInformation Resourcevivo:linkAnchorTextlink anchor textvivo:linkedAuthorlinked authorvivo:linkedInformationResourcelinked information resourcevivo:mailingAddressmailing addressvivo:overviewoverviewvivo:personInPositionpositionsPositionvivo:positionInOrganizationposition in organizationvivo:preferredTitlepreferred titleResearcher Rolevivo:researcherRoleOfresearcher role ofRolevivo:roleContributesTocontributes tovivo:sponsorAwardIdsponsor award idURLLinkvivo:webpagewebpagerdf:predicatepredicaterdf:typetyperdfs:labellabelConceptAgentfoaf:firstNamefirst namefoaf:lastNamelast nameOrganizationPersonAuthorship 1014827028121514Lopez-Rivera E, Liu YP, Verbitsky M, Anderson BR, Capone VP, Otto EA, Yan Z, Mitrotti A, Martino J, Steers NJ, Fasel DA, Vukojevic K, Deng R, Racedo SE, Liu Q, Werth M, Westland R, Vivante A, Makar GS, Bodria M, Sampson MG, Gillies CE, Vega-Warner V, Maiorana M, Petrey DS, Honig B, Lozanovski VJ, Salomon R, Heidet L, Carpentier W, Gaillard D, Carrea A, Gesualdo L, Cusi D, Izzi C, Scolari F, van Wijk JA, Arapovic A, Saraga-Babic M, Saraga M, Kunac N, Samii A, McDonald-McGinn DM, Crowley TB, Zackai EH, Drozdz D, Miklaszewska M, Tkaczyk M, Sikora P, Szczepanska M, Mizerska-Wasiak M, Krzemien G, Szmigielska A, Zaniew M, Darlow JM, Puri P, Barton D, Casolari E, Furth SL, Warady BA, Gucev Z, Hakonarson H, Flogelova H, Tasic V, Latos-Bielenska A, Materna-Kiryluk A, Allegri L, Wong CS, Drummond IA, D'Agati V, Imamoto A, Barasch JM, Hildebrandt F, Kiryluk K, Lifton RP, Morrow BE, Jeanpierre C, Papaioannou VE, Ghiggeri GM, Gharavi AG, Katsanis N, Sanna-Cherchi SThe New England journal of medicineGenetic Drivers of Kidney Defects in the DiGeorge Syndrome. N Engl J Med. 2017 02 23; 376(8):742-754.N Engl J Med2017-01-25T00:00:002017Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.Authorship 103751328439006Haller M, Mo Q, Imamoto A, Lamb DJProceedings of the National Academy of Sciences of the United States of AmericaMurine model indicates 22q11.2 signaling adaptor CRKL is a dosage-sensitive regulator of genitourinary development. Proc Natl Acad Sci U S A. 2017 05 09; 114(19):4981-4986.Proc Natl Acad Sci U S A2017-04-24T00:00:002017Murine model indicates 22q11.2 signaling adaptor CRKL is a dosage-sensitive regulator of genitourinary development.D004062Disorders9140.982025DiGeorge SyndromeRadiation and Cellular Oncology03/1988Osaka UniversityOsaka, JapanOsaka University, PhD03/1984Osaka UniversityOsaka, JapanOsaka University, DDS12/1996Fred Hutchinson Cancer Res CtrSeattle, WAFred Hutchinson Cancer Res Ctr, Post-docAuthorship 114891Authorship 106471Authorship 117483Authorship 133292Authorship 15402Authorship 146685Authorship 202128Authorship 181499Authorship 199687Authorship 200423Authorship 19911Authorship 246015Authorship 236682Authorship 286757Authorship 297298Authorship 235344Authorship 25511Authorship 286764Authorship 360532Authorship 423578Authorship 384567Authorship 33941Authorship 135805132041892Imamoto A, Ki S, Li L, Iwamoto K, Maruthamuthu V, Devany J, Lu O, Kanazawa T, Zhang S, Yamada T, Hirayama A, Fukuda S, Suzuki Y, Okada MLife science allianceEssential role of the Crk family-dosage in DiGeorge-like anomaly and metabolic homeostasis. Life Sci Alliance. 2020 02; 3(2).Life Sci Alliance2020-02-10T00:00:002020Essential role of the Crk family-dosage in DiGeorge-like anomaly and metabolic homeostasis.Authorship 376515Authorship 91193Authorship 92851Authorship 186465Authorship 223534Authorship 2924938472338Imamoto A, Wang XJ, Fujiki H, Walker SE, Beltrán LM, DiGiovanni JCarcinogenesisComparison of 12-O-tetradecanoylphorbol-13-acetate and teleocidin for induction of epidermal hyperplasia, activation of epidermal PKC isozymes and skin tumor promotion in SENCAR and C57BL/6 mice. Carcinogenesis. 1993 Apr; 14(4):719-24.Carcinogenesis1993-04-01T00:00:001993Comparison of 12-O-tetradecanoylphorbol-13-acetate and teleocidin for induction of epidermal hyperplasia, activation of epidermal PKC isozymes and skin tumor promotion in SENCAR and C57BL/6 mice.9108056Zambrowicz BP, Imamoto A, Fiering S, Herzenberg LA, Kerr WG, Soriano PProceedings of the National Academy of Sciences of the United States of AmericaDisruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proc Natl Acad Sci U S A. 1997 Apr 15; 94(8):3789-94.Proc Natl Acad Sci U S A1997-04-15T00:00:001997Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells.1576703DiGiovanni J, Imamoto A, Naito M, Walker SE, Beltrán L, Chenicek KJ, Skow LCarcinogenesisFurther genetic analyses of skin tumor promoter susceptibility using inbred and recombinant inbred mice. Carcinogenesis. 1992 Apr; 13(4):525-31.Carcinogenesis1992-04-01T00:00:001992Further genetic analyses of skin tumor promoter susceptibility using inbred and recombinant inbred mice.11242111Guris DL, Fantes J, Tara D, Druker BJ, Imamoto ANature geneticsMice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. Nat Genet. 2001 Mar; 27(3):293-8.Nat Genet2001-03-01T00:00:002001Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome.9582366Tsuda M, Matozaki T, Fukunaga K, Fujioka Y, Imamoto A, Noguchi T, Takada T, Yamao T, Takeda H, Ochi F, Yamamoto T, Kasuga MThe Journal of biological chemistryIntegrin-mediated tyrosine phosphorylation of SHPS-1 and its association with SHP-2. Roles of Fak and Src family kinases. J Biol Chem. 1998 May 22; 273(21):13223-9.J Biol Chem1998-05-22T00:00:001998Integrin-mediated tyrosine phosphorylation of SHPS-1 and its association with SHP-2. Roles of Fak and Src family kinases.11784865Hagel M, George EL, Kim A, Tamimi R, Opitz SL, Turner CE, Imamoto A, Thomas SMMolecular and cellular biologyThe adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling. Mol Cell Biol. 2002 Feb; 22(3):901-15.Mol Cell Biol2002-02-01T00:00:002002The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling.11809811Li L, Okura M, Imamoto AMolecular and cellular biologyFocal adhesions require catalytic activity of Src family kinases to mediate integrin-matrix adhesion. Mol Cell Biol. 2002 Feb; 22(4):1203-17.Mol Cell Biol2002-02-01T00:00:002002Focal adhesions require catalytic activity of Src family kinases to mediate integrin-matrix adhesion.11071910Dey A, She H, Kim L, Boruch A, Guris DL, Carlberg K, Sebti SM, Woodley DT, Imamoto A, Li WMolecular biology of the cellColony-stimulating factor-1 receptor utilizes multiple signaling pathways to induce cyclin D2 expression. Mol Biol Cell. 2000 Nov; 11(11):3835-48.Mol Biol Cell2000-11-01T00:00:002000Colony-stimulating factor-1 receptor utilizes multiple signaling pathways to induce cyclin D2 expression.11866427Lekmine F, Sassano A, Uddin S, Majchrzak B, Miura O, Druker BJ, Fish EN, Imamoto A, Platanias LCBiochemical and biophysical research communicationsThe CrkL adapter protein is required for type I interferon-dependent gene transcription and activation of the small G-protein Rap1. Biochem Biophys Res Commun. 2002 Mar 08; 291(4):744-50.Biochem Biophys Res Commun2002-03-08T00:00:002002The CrkL adapter protein is required for type I interferon-dependent gene transcription and activation of the small G-protein Rap1.14515252Peterson AC, Marks RE, Fields PE, Imamoto A, Gajewski TFEuropean journal of immunologyT cell development and function in CrkL-deficient mice. Eur J Immunol. 2003 Oct; 33(10):2687-95.Eur J Immunol2003-10-01T00:00:002003T cell development and function in CrkL-deficient mice.12665586Li L, Guris DL, Okura M, Imamoto AMolecular and cellular biologyTranslocation of CrkL to focal adhesions mediates integrin-induced migration downstream of Src family kinases. Mol Cell Biol. 2003 Apr; 23(8):2883-92.Mol Cell Biol2003-04-01T00:00:002003Translocation of CrkL to focal adhesions mediates integrin-induced migration downstream of Src family kinases.14576066Duan LJ, Imamoto A, Fong GHBloodDual roles of the C-terminal Src kinase (Csk) during developmental vascularization. Blood. 2004 Feb 15; 103(4):1370-2.Blood2003-10-23T00:00:002003Dual roles of the C-terminal Src kinase (Csk) during developmental vascularization.16574955Lee BC, Avraham S, Imamoto A, Avraham HKBloodIdentification of the nonreceptor tyrosine kinase MATK/CHK as an essential regulator of immune cells using Matk/CHK-deficient mice. Blood. 2006 Aug 01; 108(3):904-7.Blood2006-03-30T00:00:002006Identification of the nonreceptor tyrosine kinase MATK/CHK as an essential regulator of immune cells using Matk/CHK-deficient mice.Ben May Department of Cancer ResearchOrthopedic Surgery and Rehabilitation Medicine16399080Guris DL, Duester G, Papaioannou VE, Imamoto ADevelopmental cellDose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev Cell. 2006 Jan; 10(1):81-92.Dev Cell2006-01-01T00:00:002006Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome.Authorship 145063833768715Kanazawa T, Michida H, Uchino Y, Ishihara A, Zhang S, Tabata S, Suzuki Y, Imamoto A, Okada MThe FEBS journalCell shape-based chemical screening reveals an epigenetic network mediated by focal adhesions. FEBS J. 2021 10; 288(19):5613-5628.FEBS J2021-04-22T00:00:002021Cell shape-based chemical screening reveals an epigenetic network mediated by focal adhesions.19307307Seo JH, Suenaga A, Hatakeyama M, Taiji M, Imamoto AMolecular and cellular biologyStructural and functional basis of a role for CRKL in a fibroblast growth factor 8-induced feed-forward loop. Mol Cell Biol. 2009 Jun; 29(11):3076-87.Mol Cell Biol2009-03-23T00:00:002009Structural and functional basis of a role for CRKL in a fibroblast growth factor 8-induced feed-forward loop.16399079Moon AM, Guris DL, Seo JH, Li L, Hammond J, Talbot A, Imamoto ADevelopmental cellCrkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev Cell. 2006 Jan; 10(1):71-80.Dev Cell2006-01-01T00:00:002006Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes.16740711Zhu F, Choi BY, Ma WY, Zhao Z, Zhang Y, Cho YY, Choi HS, Imamoto A, Bode AM, Dong ZCancer researchCOOH-terminal Src kinase-mediated c-Jun phosphorylation promotes c-Jun degradation and inhibits cell transformation. Cancer Res. 2006 Jun 01; 66(11):5729-36.Cancer Res2006-06-01T00:00:002006COOH-terminal Src kinase-mediated c-Jun phosphorylation promotes c-Jun degradation and inhibits cell transformation.20807813Seo JH, Wood LJ, Agarwal A, O'Hare T, Elsea CR, Griswold IJ, Deininger MW, Imamoto A, Druker BJCancer researchA specific need for CRKL in p210BCR-ABL-induced transformation of mouse hematopoietic progenitors. Cancer Res. 2010 Sep 15; 70(18):7325-35.Cancer Res2010-08-31T00:00:002010A specific need for CRKL in p210BCR-ABL-induced transformation of mouse hematopoietic progenitors.18781826Zeng L, Imamoto A, Rosner MRExpert opinion on therapeutic targetsRaf kinase inhibitory protein (RKIP): a physiological regulator and future therapeutic target. Expert Opin Ther Targets. 2008 Oct; 12(10):1275-87.Expert Opin Ther Targets2008-10-01T00:00:002008Raf kinase inhibitory protein (RKIP): a physiological regulator and future therapeutic target.2401043Imamoto A, Beltrán LM, DiGiovanni JCarcinogenesisDifferential mechanism for the inhibition of epidermal growth factor binding to its receptor on mouse keratinocytes by anthrones and phorbol esters. Carcinogenesis. 1990 Sep; 11(9):1543-9.Carcinogenesis1990-09-01T00:00:001990Differential mechanism for the inhibition of epidermal growth factor binding to its receptor on mouse keratinocytes by anthrones and phorbol esters.2009135Imamoto A, Beltrán LM, DiGiovanni JMolecular carcinogenesisEvidence for autocrine/paracrine growth stimulation by transforming growth factor-alpha during the process of skin tumor promotion. Mol Carcinog. 1991; 4(1):52-60.Mol Carcinog1991-01-01T00:00:001991Evidence for autocrine/paracrine growth stimulation by transforming growth factor-alpha during the process of skin tumor promotion.D050737Chemicals & Drugs590.992808Proto-Oncogene Proteins c-crk7685657Imamoto A, Soriano PCellDisruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice. Cell. 1993 Jun 18; 73(6):1117-24.Cell1993-06-18T00:00:001993Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice.8193538Imamoto A, Soriano P, Stein PLCurrent opinion in genetics & developmentGenetics of signal transduction: tales from the mouse. Curr Opin Genet Dev. 1994 Feb; 4(1):40-6.Curr Opin Genet Dev1994-02-01T00:00:001994Genetics of signal transduction: tales from the mouse.19551145Shemon AN, Eves EM, Clark MC, Heil G, Granovsky A, Zeng L, Imamoto A, Koide S, Rosner MRPloS oneRaf Kinase Inhibitory Protein protects cells against locostatin-mediated inhibition of migration. PLoS One. 2009 Jun 24; 4(6):e6028.PLoS One2009-06-24T00:00:002009Raf Kinase Inhibitory Protein protects cells against locostatin-mediated inhibition of migration.8562483Xian W, Kiguchi K, Imamoto A, Rupp T, Zilberstein A, DiGiovanni JCell growth & differentiation : the molecular biology journal of the American Association for Cancer ResearchActivation of the epidermal growth factor receptor by skin tumor promoters and in skin tumors from SENCAR mice. Cell Growth Differ. 1995 Nov; 6(11):1447-55.Cell Growth Differ1995-11-01T00:00:001995Activation of the epidermal growth factor receptor by skin tumor promoters and in skin tumors from SENCAR mice.1740006Imamoto A, Beltrán LM, Fujiki H, Chenicek KJ, DiGiovanni JCarcinogenesisEnhanced induction of epidermal ornithine decarboxylase activity in C57BL/6 compared to DBA/2 mice by protein kinase C-activating skin tumor promoters: relevance to genetically mediated differences in promotion susceptibility. Carcinogenesis. 1992 Feb; 13(2):177-82.Carcinogenesis1992-02-01T00:00:001992Enhanced induction of epidermal ornithine decarboxylase activity in C57BL/6 compared to DBA/2 mice by protein kinase C-activating skin tumor promoters: relevance to genetically mediated differences in promotion susceptibility.15062102Ballif BA, Arnaud L, Arthur WT, Guris D, Imamoto A, Cooper JACurrent biology : CBActivation of a Dab1/CrkL/C3G/Rap1 pathway in Reelin-stimulated neurons. Curr Biol. 2004 Apr 06; 14(7):606-10.Curr Biol2004-04-06T00:00:002004Activation of a Dab1/CrkL/C3G/Rap1 pathway in Reelin-stimulated neurons.7617039Thomas SM, Soriano P, Imamoto ANatureSpecific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature. 1995 Jul 20; 376(6537):267-71.Nature1995-07-20T00:00:001995Specific and redundant roles of Src and Fyn in organizing the cytoskeleton.D048868Chemicals & Drugs1653020.849232Adaptor Proteins, Signal Transducinghttp://www.boneandcancer.orgLab homepagehttps://scholar.google.com/citations?user=YRifvyAAAAAJ&hl=enGoogle Scholarhttps://benmay.uchicago.edu/faculty/imamotoBenMayhttps://rosnerlab.comRosner Labhttp://voices.uchicago.edu/wtang-lab/Wei-Jen Tang laboratory site2010-06-30NIHIMAMOTO, AKIRA2004-07-01Developmental/Genetic Analysis of DiGeoge ModelsR01DE015883Principal InvestigatorD009687Chemicals & Drugs2646940.701846Nuclear ProteinsD015398Physiology69932330.460071Signal TransductionMedicinePathologyUniversity of ChicagoXiaoyangWuXiaoyang Wu41.78927490000000-87.601250000000001066Wu, XiaoyangAssociate ProfessorJi-HeuiSeoJi-Heui Seo41.78927490000000-87.601250000000001376Seo, Ji-Heui2.397070.00492765286research areas0.4506850.06040114coauthor of18.06723.9594560similar to1134selected publicationsCatherine ReardonAlulisCatherine Reardon Alulis41.78927490000000-87.60125000000000178Alulis, Catherine ReardonResearch ProfessorRaviSalgiaRavi Salgia41.78927490000000-87.601250000000001937Salgia, RaviEmeritus/Emerita, ProfessorRalph R.WeichselbaumxXB+DqgWEf/5hHlsaUXv9Gq0+s+oRalph R. Weichselbaum41.78927490000000-87.601250000000002521Weichselbaum, Ralph R.ProfessorMarshaRosnerMarsha Rosner41.78927490000000-87.60125000000000257Rosner, MarshaProfessorThomas F.GajewskiThomas F. Gajewski41.78927490000000-87.60125000000000355Gajewski, Thomas F.ProfessorTong-ChuanHew2FhDoEXCuiXhHlsaUXv9Gq0+s+oTong-Chuan He41.78927490000000-87.60125000000000550He, Tong-ChuanAssociate ProfessorYusukeNakamuraYusuke Nakamura41.78927490000000-87.60125000000000582Nakamura, YusukeEmeritus/Emerita, ProfessorAnningLinAnning Lin41.78927490000000-87.60125000000000677Lin, AnningProfessorLeiLiLei Li41.78927490000000-87.6012500000000089Li, LeiWei-JenTangWei-Jen Tang41.78927490000000-87.60125000000000957Tang, Wei-JenProfessortrue1true1true1ProfessorProfessortrue1Research ProfessorResearch Professortrue1ProfessorProfessortrue1ProfessorProfessortrue1Associate ProfessorAssociate Professortrue1Associate ProfessorAssociate Professortrue1Emeritus/Emerita, ProfessorEmeritus/Emerita, Professortrue1Emeritus/Emerita, ProfessorEmeritus/Emerita, Professortrue1Associate ProfessorAssociate Professortrue1ProfessorProfessortrue1ProfessorProfessorChicago60637ILAuthorship 61542823416466Zeng L, Ehrenreiter K, Menon J, Menard R, Kern F, Nakazawa Y, Bevilacqua E, Imamoto A, Baccarini M, Rosner MRCellular signallingRKIP regulates MAP kinase signaling in cells with defective B-Raf activity. Cell Signal. 2013 May; 25(5):1156-65.Cell Signal2013-02-14T00:00:002013RKIP regulates MAP kinase signaling in cells with defective B-Raf activity.Authorship 952341327686861Shigeno-Nakazawa Y, Kasai T, Ki S, Kostyanovskaya E, Pawlak J, Yamagishi J, Okimoto N, Taiji M, Okada M, Westbrook J, Satta Y, Kigawa T, Imamoto AScientific reportsA pre-metazoan origin of the CRK gene family and co-opted signaling network. Sci Rep. 2016 Sep 30; 6:34349.Sci Rep2016-09-30T00:00:002016A pre-metazoan origin of the CRK gene family and co-opted signaling network.