WilliamGreenWilliam Green41.78927490000000-87.60125000000000303Green, WilliamAcetylcholineAcetylcholine Receptors, NicotinicGlutamateGlutamate Receptors, IonotropicSynapsesTrafficking, ProteinCell Biology of NeuronsNicotineNicotine AddictionMy research is focused on ionotropic neurotransmitter receptors, the receptors responsible for the rapid postsynaptic response in nerve and muscle. These receptors are large oligomeric membrane proteins with subunits surrounding an ion channel that opens when neurotransmitters bind to the receptor. There are two different families of ionotropic neurotransmitter receptors. One family includes nicotinic acetylcholine receptors (AChRs), GABA and glycine receptors, and the other family are glutamate receptors, both NMDA- and AMPA-type glutamate receptors. The overall goal of my research is to understand how nerve and muscle build these receptors and traffic them specifically to and from synapses. These events regulate the number, density and function of the receptors at synapses, which helps define synaptic strength. The same events underlie learning and memory formation, and when they fail, can contribute to a number of diseases including Alzheimer’s disease, Huntington’s Disease, Myasthenia Gravis and Myasthenic Syndromes.
There are several projects ongoing in my lab characterizing the basic cell biology of these receptors, which include receptor assembly, trafficking and clustering. Assembly refers to the processes that transform newly synthesized subunits into functional receptors usually in the endoplasmic reticulum. Trafficking refers to the processes that transport the receptors to and from different location in cells and targets them to these locations. Clustering is the process that packs and maintains the receptors in regions of high density such as synapses. Recently, we have developed new techniques for assaying the protein post-translation modification known as palmitoylation. This work has led to several collaborations in which we are helping to characterize the palmitoylation of a number of different proteins. I also am collaborating with Dr. Paul Selvin (University of Illinois) developing fluorescent single-molecule methods to characterize neurotransmitter receptor subunit composition, stoichiometry and the diffusion/trafficking of these receptors.Professorplugins:FeaturedVideosFeatured Videosplugins:TwitterTwitterprns:coAuthorOfcoauthor ofprns:emailEncryptedemail addressprns:endDateend dateprns:fullNamefull nameprns:grantAwardedBygrant awarded byprns:hasAuthorListauthor listprns:hasNetworkhas networkprns:hasPublicationVenuepublished inprns:informationResourceReferenceinformation resource referenceprns:isPrimaryPositionis primary positionprns:latitudelatitudeprns:longitudelongitudeprns:mainImagephotoprns:maxWeightmaximum weightprns:medlineTAjournal title abbreviationprns:meshDescriptorUIMeSH DescriptorUIprns:meshSemanticGroupNameMeSH semantic group nameprns:minWeightminimum weightprns:numberOfAuthorsnumber of authorsprns:numberOfConnectionsnumber of connectionsprns:numberOfPublicationsnumber of publicationsprns:personIdPerson IDprns:personInPrimaryPositionperson in primary positionprns:physicalNeighborOfphysical neighborprns:pluginSearchableDataProfilesRNS Plugin Searchable Dataprns:positionInDepartmentposition in departmentprns:predicateNodepredicate nodeprns:principalInvestigatorNameprincipal investigator nameprns:publicationDatepublication dateprns:similarTosimilar toprns:sortOrdersort orderprns:startDatestart dateprns:trainingAtOrganizationeducational organizationprns:trainingLocationtraining locationprns:uniquenessWeightuniqueness weightprns:yearyearAcademic ArticleArticleDocumentbibo:pmidPubMed IdentifierAddressvivo:addressCitycityvivo:addressPostalCodepostal codevivo:addressStatestate or provinceAgreementvivo:authorInAuthorshipselected publicationsvivo:authorRankauthor rank in publicationAuthorshipvivo:degreeEarneddegree earnedDepartmentvivo:educationalTrainingeducation and trainingEducational Trainingvivo:freetextKeywordkeywordsGrantvivo:hasResearchArearesearch areasvivo:hasResearcherRoleresearch activitiesvivo:hrJobTitleHR job titleInformation Resourcevivo:linkAnchorTextlink anchor textvivo:linkedAuthorlinked authorvivo:linkedInformationResourcelinked information resourcevivo:mailingAddressmailing addressvivo:majorFieldmajor field of degreevivo:overviewoverviewvivo:personInPositionpositionsPositionvivo:positionInOrganizationposition in organizationvivo:preferredTitlepreferred titleResearcher Rolevivo:researcherRoleOfresearcher role ofRolevivo:roleContributesTocontributes tovivo:sponsorAwardIdsponsor award idURLLinkvivo:webpagewebpagerdf:predicatepredicaterdf:typetyperdfs:labellabelConceptAgentfoaf:firstNamefirst namefoaf:lastNamelast nameOrganizationPerson06/1986Cornell University Graduate School of Medical SciencesNew York, NYCornell University Graduate School of Medical Sciences, Physiology & BiophysicsPh.D.Physiology & Biophysics06/1978University College, University of TorontoToronto, Canada University College, University of Toronto, Physics & ZoologyB.Sc. Physics & Zoology12/1992Yale UniversityNew Haven, CTYale University, Physiology & NeurosciencePostdoctoral FellowPhysiology & NeuroscienceAuthorship 1077916Authorship 10779215Authorship 10779315Authorship 1077946Authorship 1077959Authorship 10779616Authorship 10779715Authorship 1077986Authorship 10779912Authorship 10780010Authorship 1078019Authorship 10780211Authorship 1078039Authorship 1078043Authorship 1078056Authorship 10780612Authorship 1078072Authorship 1078083Authorship 1078092Authorship 1078101Authorship 1078111Authorship 1078124Authorship 1078133Authorship 1078142Authorship 1078151Authorship 1078161Authorship 1078173Authorship 1078183Authorship 1078192Authorship 1078201Authorship 1078211Authorship 107822119556522Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MRCirculation researchPalmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 2009 Jul 17; 105(2):138-47.Circ Res2009-06-25T00:00:002009Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function.18434415Liu Y, Padgett D, Takahashi M, Li H, Sayeed A, Teichert RW, Olivera BM, McArdle JJ, Green WN, Lin WDevelopment (Cambridge, England)Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning. Development. 2008 Jun; 135(11):1957-67.Development2008-04-23T00:00:002008Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning.8334706Green WN, Claudio TCellAcetylcholine receptor assembly: subunit folding and oligomerization occur sequentially. Cell. 1993 Jul 16; 74(1):57-69.Cell1993-07-16T00:00:001993Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially.1658190Chabala LD, Urban BW, Weiss LB, Green WN, Andersen OSThe Journal of general physiologySteady-state gating of batrachotoxin-modified sodium channels. Variability and electrolyte-dependent modulation. J Gen Physiol. 1991 Jul; 98(1):197-224.J Gen Physiol1991-07-01T00:00:001991Steady-state gating of batrachotoxin-modified sodium channels. Variability and electrolyte-dependent modulation.1849906Ross AF, Green WN, Hartman DS, Claudio TThe Journal of cell biologyEfficiency of acetylcholine receptor subunit assembly and its regulation by cAMP. J Cell Biol. 1991 May; 113(3):623-36.J Cell Biol1991-05-01T00:00:001991Efficiency of acetylcholine receptor subunit assembly and its regulation by cAMP.2359035Hartman DS, Poo MM, Green WN, Ross AF, Claudio TJournal de physiologieSynaptic contact between embryonic neurons and acetylcholine receptor-fibroblast. J Physiol (Paris). 1990; 84(1):42-9.J Physiol (Paris)1990-01-01T00:00:001990Synaptic contact between embryonic neurons and acetylcholine receptor-fibroblast.2440978Green WN, Weiss LB, Andersen OSThe Journal of general physiologyBatrachotoxin-modified sodium channels in planar lipid bilayers. Characterization of saxitoxin- and tetrodotoxin-induced channel closures. J Gen Physiol. 1987 Jun; 89(6):873-903.J Gen Physiol1987-06-01T00:00:001987Batrachotoxin-modified sodium channels in planar lipid bilayers. Characterization of saxitoxin- and tetrodotoxin-induced channel closures.3468845Green WN, Andersen OSAnnals of the New York Academy of SciencesSurface charges near the guanidinium neurotoxin binding site. Ann N Y Acad Sci. 1986; 479:306-12.Ann N Y Acad Sci1986-01-01T00:00:001986Surface charges near the guanidinium neurotoxin binding site.29277457Samra GK, Intskirveli I, Govind AP, Liang C, Lazar R, Green WN, Metherate R, Mukherjee JBioorganic & medicinal chemistry lettersDevelopment of fluorescence imaging probes for nicotinic acetylcholine a4ß2* receptors. Bioorg Med Chem Lett. 2018 02 01; 28(3):371-377.Bioorg Med Chem Lett2017-12-17T00:00:002017Development of fluorescence imaging probes for nicotinic acetylcholine a4ß2* receptors.25397889Wang Y, Cai E, Rosenkranz T, Ge P, Teng KW, Lim SJ, Smith AM, Chung HJ, Sachs F, Green WN, Gottlieb P, Selvin PRBioconjugate chemistrySmall quantum dots conjugated to nanobodies as immunofluorescence probes for nanometric microscopy. Bioconjug Chem. 2014 Dec 17; 25(12):2205-11.Bioconjug Chem2014-11-25T00:00:002014Small quantum dots conjugated to nanobodies as immunofluorescence probes for nanometric microscopy.21775500Singaraja RR, Huang K, Sanders SS, Milnerwood AJ, Hines R, Lerch JP, Franciosi S, Drisdel RC, Vaid K, Young FB, Doty C, Wan J, Bissada N, Henkelman RM, Green WN, Davis NG, Raymond LA, Hayden MRHuman molecular geneticsAltered palmitoylation and neuropathological deficits in mice lacking HIP14. Hum Mol Genet. 2011 Oct 15; 20(20):3899-909.Hum Mol Genet2011-07-20T00:00:002011Altered palmitoylation and neuropathological deficits in mice lacking HIP14.12566536Kopito RR, Green WN, Andersen OThe Journal of general physiologyThe 56th Annual Meeting and Symposium of the Society of General Physiologists: trafficking of transporters. J Gen Physiol. 2003 Feb; 121(2):73-9.J Gen Physiol2003-02-01T00:00:002003The 56th Annual Meeting and Symposium of the Society of General Physiologists: trafficking of transporters.1931053Green WN, Ross AF, Claudio TNeuronAcetylcholine receptor assembly is stimulated by phosphorylation of its gamma subunit. Neuron. 1991 Oct; 7(4):659-66.Neuron1991-10-01T00:00:001991Acetylcholine receptor assembly is stimulated by phosphorylation of its gamma subunit.2045417Paulson HL, Ross AF, Green WN, Claudio TThe Journal of cell biologyAnalysis of early events in acetylcholine receptor assembly. J Cell Biol. 1991 Jun; 113(6):1371-84.J Cell Biol1991-06-01T00:00:001991Analysis of early events in acetylcholine receptor assembly.1846964Green WN, Ross AF, Claudio TProceedings of the National Academy of Sciences of the United States of AmericacAMP stimulation of acetylcholine receptor expression is mediated through posttranslational mechanisms. Proc Natl Acad Sci U S A. 1991 Feb 01; 88(3):854-8.Proc Natl Acad Sci U S A1991-02-01T00:00:001991cAMP stimulation of acetylcholine receptor expression is mediated through posttranslational mechanisms.1710438Green WN, Andersen OSAnnual review of physiologySurface charges and ion channel function. Annu Rev Physiol. 1991; 53:341-59.Annu Rev Physiol1991-01-01T00:00:001991Surface charges and ion channel function.2472403Claudio T, Paulson HL, Green WN, Ross AF, Hartman DS, Hayden DThe Journal of cell biologyFibroblasts transfected with Torpedo acetylcholine receptor beta-, gamma-, and delta-subunit cDNAs express functional receptors when infected with a retroviral alpha recombinant. J Cell Biol. 1989 Jun; 108(6):2277-90.J Cell Biol1989-06-01T00:00:001989Fibroblasts transfected with Torpedo acetylcholine receptor beta-, gamma-, and delta-subunit cDNAs express functional receptors when infected with a retroviral alpha recombinant.3686008Claudio T, Green WN, Hartman DS, Hayden D, Paulson HL, Sigworth FJ, Sine SM, Swedlund AScience (New York, N.Y.)Genetic reconstitution of functional acetylcholine receptor channels in mouse fibroblasts. Science. 1987 Dec 18; 238(4834):1688-94.Science1987-12-18T00:00:001987Genetic reconstitution of functional acetylcholine receptor channels in mouse fibroblasts.28718768Govind AP, Vallejo YF, Stolz JR, Yan JZ, Swanson GT, Green WNeLifeSelective and regulated trapping of nicotinic receptor weak base ligands and relevance to smoking cessation. Elife. 2017 07 18; 6.Elife2017-07-18T00:00:002017Selective and regulated trapping of nicotinic receptor weak base ligands and relevance to smoking cessation.17012025Roth AF, Wan J, Green WN, Yates JR, Davis NGMethods (San Diego, Calif.)Proteomic identification of palmitoylated proteins. Methods. 2006 Oct; 40(2):135-42.Methods2006-10-01T00:00:002006Proteomic identification of palmitoylated proteins.16699508Yanai A, Huang K, Kang R, Singaraja RR, Arstikaitis P, Gan L, Orban PC, Mullard A, Cowan CM, Raymond LA, Drisdel RC, Green WN, Ravikumar B, Rubinsztein DC, El-Husseini A, Hayden MRNature neurosciencePalmitoylation of huntingtin by HIP14 is essential for its trafficking and function. Nat Neurosci. 2006 Jun; 9(6):824-31.Nat Neurosci2006-05-14T00:00:002006Palmitoylation of huntingtin by HIP14 is essential for its trafficking and function.7962204Jayawickreme SP, Green WN, Claudio TJournal of cell scienceCyclic AMP-regulated AChR assembly is independent of AChR subunit phosphorylation by PKA. J Cell Sci. 1994 Jun; 107 ( Pt 6):1641-51.J Cell Sci1994-06-01T00:00:001994Cyclic AMP-regulated AChR assembly is independent of AChR subunit phosphorylation by PKA.29116041Lee SH, Jin C, Cai E, Ge P, Ishitsuka Y, Teng KW, de Thomaz AA, Nall D, Baday M, Jeyifous O, Demonte D, Dundas CM, Park S, Delgado JY, Green WN, Selvin PReLifeCorrection: Super-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes. Elife. 2017 11 08; 6.Elife2017-11-08T00:00:002017Correction: Super-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes.28749340Lee SH, Jin C, Cai E, Ge P, Ishitsuka Y, Teng KW, de Thomaz AA, Nall D, Baday M, Jeyifous O, Demonte D, Dundas CM, Park S, Delgado JY, Green WN, Selvin PReLifeSuper-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes. Elife. 2017 07 27; 6.Elife2017-07-27T00:00:002017Super-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes.19092927Kang R, Wan J, Arstikaitis P, Takahashi H, Huang K, Bailey AO, Thompson JX, Roth AF, Drisdel RC, Mastro R, Green WN, Yates JR, Davis NG, El-Husseini ANatureNeural palmitoyl-proteomics reveals dynamic synaptic palmitoylation. Nature. 2008 Dec 18; 456(7224):904-9.Nature2008-12-18T00:00:002008Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.16751107Roth AF, Wan J, Bailey AO, Sun B, Kuchar JA, Green WN, Phinney BS, Yates JR, Davis NGCellGlobal analysis of protein palmitoylation in yeast. Cell. 2006 Jun 02; 125(5):1003-13.Cell2006-06-02T00:00:002006Global analysis of protein palmitoylation in yeast.11044727Buisson B, Vallejo YF, Green WN, Bertrand DNeuropharmacologyThe unusual nature of epibatidine responses at the alpha4beta2 nicotinic acetylcholine receptor. Neuropharmacology. 2000 Oct; 39(13):2561-9.Neuropharmacology2000-10-01T00:00:002000The unusual nature of epibatidine responses at the alpha4beta2 nicotinic acetylcholine receptor.2440977Green WN, Weiss LB, Andersen OSThe Journal of general physiologyBatrachotoxin-modified sodium channels in planar lipid bilayers. Ion permeation and block. J Gen Physiol. 1987 Jun; 89(6):841-72.J Gen Physiol1987-06-01T00:00:001987Batrachotoxin-modified sodium channels in planar lipid bilayers. Ion permeation and block.Anesthesia and Critical CareMolecular Genetics and Cell BiologyNeurobiologyAuthorship 1100763Authorship 1117453Authorship 1111863Authorship 1140892Authorship 1164783Authorship 1167202Authorship 1225982Authorship 12175329551954Mayrand SM, Schwarz DA, Green WRJournal of immunology (Baltimore, Md. : 1950)An alternative translational reading frame encodes an immunodominant retroviral CTL determinant expressed by an immunodeficiency-causing retrovirus. J Immunol. 1998 Jan 01; 160(1):39-50.J Immunol1998-01-01T00:00:001998An alternative translational reading frame encodes an immunodominant retroviral CTL determinant expressed by an immunodeficiency-causing retrovirus.24335302O'Connor MA, Green WRJournal of virologyUse of IRF-3 and/or IRF-7 knockout mice to study viral pathogenesis: lessons from a murine retrovirus-induced AIDS model. J Virol. 2014 Feb; 88(4):2349-53.J Virol2013-12-11T00:00:002013Use of IRF-3 and/or IRF-7 knockout mice to study viral pathogenesis: lessons from a murine retrovirus-induced AIDS model.6254643Tam MR, Green WR, Nowinski RCCancer researchCytotoxic activities of monoclonal antibodies against the envelope proteins of murine leukemia virus. Cancer Res. 1980 Oct; 40(10):3850-3.Cancer Res1980-10-01T00:00:001980Cytotoxic activities of monoclonal antibodies against the envelope proteins of murine leukemia virus.21142443Carlson TL, Green KA, Green WRViral immunologyAlternative translational reading frames as a novel source of epitopes for an expanded CD8 T-cell repertoire: use of a retroviral system to assess the translational requirements for CTL recognition and lysis. Viral Immunol. 2010 Dec; 23(6):577-83.Viral Immunol2010-12-01T00:00:002010Alternative translational reading frames as a novel source of epitopes for an expanded CD8 T-cell repertoire: use of a retroviral system to assess the translational requirements for CTL recognition and lysis.12101448Ghazi NG, Green WREye (London, England)Pathology and pathogenesis of retinal detachment. Eye (Lond). 2002 Jul; 16(4):411-21.Eye (Lond)2002-07-01T00:00:002002Pathology and pathogenesis of retinal detachment.232585Wong VG, Green WR, Liu YP, Marsden ERTransactions of the American Ophthalmological SocietyExperimental intraocular malignancy: the effect of intracameral perfusion. Trans Am Ophthalmol Soc. 1979; 77:63-79.Trans Am Ophthalmol Soc1979-01-01T00:00:001979Experimental intraocular malignancy: the effect of intracameral perfusion.11190024Knox DL, Kerrison JB, Green WRTransactions of the American Ophthalmological SocietyHistopathologic studies of ischemic optic neuropathy. Trans Am Ophthalmol Soc. 2000; 98:203-20; discussion 221-2.Trans Am Ophthalmol Soc2000-01-01T00:00:002000Histopathologic studies of ischemic optic neuropathy.10889120Hayashi N, Geraghty MT, Green WROphthalmologyOcular histopathologic study of a patient with the T 8993-G point mutation in Leigh's syndrome. Ophthalmology. 2000 Jul; 107(7):1397-402.Ophthalmology2000-07-01T00:00:002000Ocular histopathologic study of a patient with the T 8993-G point mutation in Leigh's syndrome.Authorship 137351Authorship 144103Authorship 139419Authorship 156161Authorship 150573Authorship 149271Authorship 168742Authorship 161996Authorship 162342Authorship 188603Authorship 2166012Authorship 237083Authorship 244052Authorship 276752Authorship 257722Authorship 260313Authorship 332523Authorship 305684Authorship 327365Authorship 420356Authorship 389437Authorship 3670914Authorship 328152Authorship 339077D011499Physiology1523710.819179Protein Processing, Post-TranslationalAuthorship 89501Authorship 475753Authorship 139804232754027Yoshii A, Green WNFrontiers in synaptic neuroscienceEditorial: Role of Protein Palmitoylation in Synaptic Plasticity and Neuronal Differentiation. Front Synaptic Neurosci. 2020; 12:27.Front Synaptic Neurosci2020-07-09T00:00:002020Editorial: Role of Protein Palmitoylation in Synaptic Plasticity and Neuronal Differentiation.Authorship 272704Authorship 3842239334396Rangwala F, Drisdel RC, Rakhilin S, Ko E, Atluri P, Harkins AB, Fox AP, Salman SS, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceNeuronal alpha-bungarotoxin receptors differ structurally from other nicotinic acetylcholine receptors. J Neurosci. 1997 Nov 01; 17(21):8201-12.J Neurosci1997-11-01T00:00:001997Neuronal alpha-bungarotoxin receptors differ structurally from other nicotinic acetylcholine receptors.9252423Green WN, Wanamaker CPThe Journal of biological chemistryThe role of the cystine loop in acetylcholine receptor assembly. J Biol Chem. 1997 Aug 15; 272(33):20945-53.J Biol Chem1997-08-15T00:00:001997The role of the cystine loop in acetylcholine receptor assembly.9925815Green WNThe Journal of general physiologyIon channel assembly: creating structures that function. J Gen Physiol. 1999 Feb; 113(2):163-70.J Gen Physiol1999-02-01T00:00:001999Ion channel assembly: creating structures that function.9671647Green WN, Wanamaker CPThe Journal of neuroscience : the official journal of the Society for NeuroscienceFormation of the nicotinic acetylcholine receptor binding sites. J Neurosci. 1998 Aug 01; 18(15):5555-64.J Neurosci1998-08-01T00:00:001998Formation of the nicotinic acetylcholine receptor binding sites.10402471Rakhilin S, Drisdel RC, Sagher D, McGehee DS, Vallejo Y, Green WNThe Journal of cell biologyalpha-bungarotoxin receptors contain alpha7 subunits in two different disulfide-bonded conformations. J Cell Biol. 1999 Jul 12; 146(1):203-18.J Cell Biol1999-07-12T00:00:001999alpha-bungarotoxin receptors contain alpha7 subunits in two different disulfide-bonded conformations.9499800Green KA, Noelle RJ, Green WRVirologyEvidence for a continued requirement for CD40/CD40 ligand (CD154) interactions in the progression of LP-BM5 retrovirus-induced murine AIDS. Virology. 1998 Feb 15; 241(2):260-8.Virology1998-02-15T00:00:001998Evidence for a continued requirement for CD40/CD40 ligand (CD154) interactions in the progression of LP-BM5 retrovirus-induced murine AIDS.Authorship 141788833112725Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BSMolecular biology of the cellESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Mol Biol Cell. 2020 12 15; 31(26):2892-2903.Mol Biol Cell2020-10-28T00:00:002020ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.9711629Eertmoed AL, Vallejo YF, Green WNMethods in enzymologyTransient expression of heteromeric ion channels. Methods Enzymol. 1998; 293:564-85.Methods Enzymol1998-01-01T00:00:001998Transient expression of heteromeric ion channels.10627589Drisdel RC, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceNeuronal alpha-bungarotoxin receptors are alpha7 subunit homomers. J Neurosci. 2000 Jan 01; 20(1):133-9.J Neurosci2000-01-01T00:00:002000Neuronal alpha-bungarotoxin receptors are alpha7 subunit homomers.D018722Chemicals & Drugs30720.921427Nicotinic Agonists12460547Leem JY, Saura CA, Pietrzik C, Christianson J, Wanamaker C, King LT, Veselits ML, Tomita T, Gasparini L, Iwatsubo T, Xu H, Green WN, Koo EH, Thinakaran GNeurobiology of diseaseA role for presenilin 1 in regulating the delivery of amyloid precursor protein to the cell surface. Neurobiol Dis. 2002 Oct; 11(1):64-82.Neurobiol Dis2002-10-01T00:00:002002A role for presenilin 1 in regulating the delivery of amyloid precursor protein to the cell surface.14989092Drisdel RC, Green WNBioTechniquesLabeling and quantifying sites of protein palmitoylation. Biotechniques. 2004 Feb; 36(2):276-85.Biotechniques2004-02-01T00:00:002004Labeling and quantifying sites of protein palmitoylation.14592864Wanamaker CP, Christianson JC, Green WNAnnals of the New York Academy of SciencesRegulation of nicotinic acetylcholine receptor assembly. Ann N Y Acad Sci. 2003 Sep; 998:66-80.Ann N Y Acad Sci2003-09-01T00:00:002003Regulation of nicotinic acetylcholine receptor assembly.15944384Vallejo YF, Buisson B, Bertrand D, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceChronic nicotine exposure upregulates nicotinic receptors by a novel mechanism. J Neurosci. 2005 Jun 08; 25(23):5563-72.J Neurosci2005-06-08T00:00:002005Chronic nicotine exposure upregulates nicotinic receptors by a novel mechanism.16091366Wanamaker CP, Green WNThe Journal of biological chemistryN-linked glycosylation is required for nicotinic receptor assembly but not for subunit associations with calnexin. J Biol Chem. 2005 Oct 07; 280(40):33800-10.J Biol Chem2005-08-09T00:00:002005N-linked glycosylation is required for nicotinic receptor assembly but not for subunit associations with calnexin.17012024Drisdel RC, Alexander JK, Sayeed A, Green WNMethods (San Diego, Calif.)Assays of protein palmitoylation. Methods. 2006 Oct; 40(2):127-34.Methods2006-10-01T00:00:002006Assays of protein palmitoylation.15483627Christianson JC, Green WNThe EMBO journalRegulation of nicotinic receptor expression by the ubiquitin-proteasome system. EMBO J. 2004 Oct 27; 23(21):4156-65.EMBO J2004-10-14T00:00:002004Regulation of nicotinic receptor expression by the ubiquitin-proteasome system.17936462Vezina P, McGehee DS, Green WNProgress in neuro-psychopharmacology & biological psychiatryExposure to nicotine and sensitization of nicotine-induced behaviors. Prog Neuropsychopharmacol Biol Psychiatry. 2007 Nov 15; 31(8):1625-38.Prog Neuropsychopharmacol Biol Psychiatry2007-09-01T00:00:002007Exposure to nicotine and sensitization of nicotine-induced behaviors.19028695Cheng H, Vetrivel KS, Drisdel RC, Meckler X, Gong P, Leem JY, Li T, Carter M, Chen Y, Nguyen P, Iwatsubo T, Tomita T, Wong PC, Green WN, Kounnas MZ, Thinakaran GThe Journal of biological chemistryS-palmitoylation of gamma-secretase subunits nicastrin and APH-1. J Biol Chem. 2009 Jan 16; 284(3):1373-84.J Biol Chem2008-11-20T00:00:002008S-palmitoylation of gamma-secretase subunits nicastrin and APH-1.20668195Alexander JK, Sagher D, Krivoshein AV, Criado M, Jefford G, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceRic-3 promotes alpha7 nicotinic receptor assembly and trafficking through the ER subcompartment of dendrites. J Neurosci. 2010 Jul 28; 30(30):10112-26.J Neurosci2010-07-28T00:00:002010Ric-3 promotes alpha7 nicotinic receptor assembly and trafficking through the ER subcompartment of dendrites.17702741Drisdel RC, Sharp D, Henderson T, Hales TG, Green WNThe Journal of biological chemistryHigh affinity binding of epibatidine to serotonin type 3 receptors. J Biol Chem. 2008 Apr 11; 283(15):9659-65.J Biol Chem2007-08-15T00:00:002007High affinity binding of epibatidine to serotonin type 3 receptors.18174175Walsh H, Govind AP, Mastro R, Hoda JC, Bertrand D, Vallejo Y, Green WNThe Journal of biological chemistryUp-regulation of nicotinic receptors by nicotine varies with receptor subtype. J Biol Chem. 2008 Mar 07; 283(10):6022-32.J Biol Chem2008-01-03T00:00:002008Up-regulation of nicotinic receptors by nicotine varies with receptor subtype.17728248Wanamaker CP, Green WNThe Journal of biological chemistryEndoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly. J Biol Chem. 2007 Oct 26; 282(43):31113-23.J Biol Chem2007-08-29T00:00:002007Endoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly.22323734Govind AP, Walsh H, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceNicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms. J Neurosci. 2012 Feb 08; 32(6):2227-38.J Neurosci2012-02-08T00:00:002012Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.19540212Govind AP, Vezina P, Green WNBiochemical pharmacologyNicotine-induced upregulation of nicotinic receptors: underlying mechanisms and relevance to nicotine addiction. Biochem Pharmacol. 2009 Oct 01; 78(7):756-65.Biochem Pharmacol2009-06-18T00:00:002009Nicotine-induced upregulation of nicotinic receptors: underlying mechanisms and relevance to nicotine addiction.D018091Chemicals & Drugs31660.956249Receptors, AMPA7571003Green WN, Millar NSTrends in neurosciencesIon-channel assembly. Trends Neurosci. 1995 Jun; 18(6):280-7.Trends Neurosci1995-06-01T00:00:001995Ion-channel assembly.Authorship 1498641534545811Govind AP, Jeyifous O, Russell TA, Yi Z, Weigel AV, Ramaprasad A, Newell L, Ramos W, Valbuena FM, Casler JC, Yan JZ, Glick BS, Swanson GT, Lippincott-Schwartz J, Green WNeLifeActivity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome. Elife. 2021 09 21; 10.Elife2021-09-21T00:00:002021Activity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome.19693711Alexander JK, Govind AP, Drisdel RC, Blanton MP, Vallejo Y, Lam TT, Green WNJournal of molecular neuroscience : MNPalmitoylation of nicotinic acetylcholine receptors. J Mol Neurosci. 2010 Jan; 40(1-2):12-20.J Mol Neurosci2009-08-20T00:00:002009Palmitoylation of nicotinic acetylcholine receptors.10414959Eertmoed AL, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceNicotinic receptor assembly requires multiple regions throughout the gamma subunit. J Neurosci. 1999 Aug 01; 19(15):6298-308.J Neurosci1999-08-01T00:00:001999Nicotinic receptor assembly requires multiple regions throughout the gamma subunit.11312284Mitra M, Wanamaker CP, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceRearrangement of nicotinic receptor alpha subunits during formation of the ligand binding sites. J Neurosci. 2001 May 01; 21(9):3000-8.J Neurosci2001-05-01T00:00:002001Rearrangement of nicotinic receptor alpha subunits during formation of the ligand binding sites.15548665Drisdel RC, Manzana E, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceThe role of palmitoylation in functional expression of nicotinic alpha7 receptors. J Neurosci. 2004 Nov 17; 24(46):10502-10.J Neurosci2004-11-17T00:00:002004The role of palmitoylation in functional expression of nicotinic alpha7 receptors.Authorship 150648634701634Salerno M, Williams GR, Scheuermann RM, Kennedy C, Agarwal M, Green WR, Gu W, Zhang Z, Lin H, Carlson DJ, Dong L, Metz JM, Li TInternational journal of radiation oncology, biology, physicsDeploying a Single-Energy O-Ring Linac as the Sole Treatment Machine in a Community Setting: A Feasibility Study. Int J Radiat Oncol Biol Phys. 2021 Nov 01; 111(3S):e514.Int J Radiat Oncol Biol Phys2021-11-01T00:00:002021Deploying a Single-Energy O-Ring Linac as the Sole Treatment Machine in a Community Setting: A Feasibility Study.D011978Chemicals & Drugs401290.905687Receptors, NicotinicAuthorship 1587631336028313Zhang HJ, Zammit M, Kao CM, Govind AP, Mitchell S, Holderman N, Bhuiyan M, Freifelder R, Kucharski A, Zhuang X, Mukherjee J, Chen CT, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceTrapping of Nicotinic Acetylcholine Receptor Ligands Assayed by In Vitro Cellular Studies and In Vivo PET Imaging. J Neurosci. 2023 01 04; 43(1):2-13.J Neurosci2022-08-26T00:00:002022Trapping of Nicotinic Acetylcholine Receptor Ligands Assayed by In Vitro Cellular Studies and In Vivo PET Imaging.https://ragsdalelab.uchicago.edu/Ragsdale Laboratoryhttp://neurobiology.uchicago.edu/page/wei-weiDepartmental Sitehttp://voices.uchicago.edu/drsisodialab/Sisodia Labhttps://cpb-us-w2.wpmucdn.com/voices.uchicago.edu/dist/e/772/files/2019/10/ACFrOgCOWaX1k-0F2phup4Kdbe_UAJxPHs-eXWdz39eZftkvJP7iTAjzrBTAsWiLe56mP9fcDklTlbjhSM51HQSgUi5KmcOHeOnzQgwUSY10fRhBD4-L14dlPBG7hpM.pdfZhuang Labhttp://glicklab.bsd.uchicago.edu/Glick Labhttp://voices.uchicago.edu/weilab/Lab Websitehttps://twitter.com/RagsdaleLab2017-09-29NIHSELVIN, PAUL R2014-09-30Organization and Dynamics of PSD-bound Glutamate Receptors at Super-resolutionR56NS0909032022-04-30NIHGREEN, WILLIAM2017-07-01PET imaging of a4b2 nicotinic receptor upregulation and smoking cessationR01DA0447602003-08-31NIHGREEN, WILLIAM N.2002-09-03Trafficking of TransportersR13DK0636462011-11-30NIHGREEN, WILLIAM NATHAN2009-12-04Proteomic Assays of Neuronal Protein PalmitoylationR21MH0812512018-04-30NIHGREEN, WILLIAM2013-08-01Different components of nicotine-induced upregulation of nicotinic receptorsR01DA0354302026-08-31NIHCHUNG, HEE JUNG ;SELVIN, PAUL R2017-07-01Super-Resolution Microscopy of Neuronal Synapses with Advanced Imaging ToolsR01NS1000192007-05-31NIHGREEN, WILLIAM N.2001-08-01UPREGULATION OF NICOTINIC RECEPTORSR01DA0136022009-07-31NIHGREEN, WILLIAM N.2002-04-01The Neuronal alpha-bungarotoxin Binding SiteR56NS0437822013-07-31NIHGREEN, WILLIAM2002-04-01The Neuronal alpha-bungarotoxin Binding SiteR01NS0437822007-03-31NIHGREEN, WILLIAM N.1997-08-01Quality Control of Nicotinic Receptor AssemblyR01NS032693Co-Principal InvestigatorPrincipal InvestigatorCo-Principal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorD009538Chemicals & Drugs571930.858565NicotineMedicineNeurologyUniversity of ChicagoRaymond P.RoosxXBmBLI1F+nMg3Vob0H3vWfp+4Wo6r1Xc3h9eaOOFmI=Raymond P. Roos41.78927490000000-87.601250000000001191Roos, Raymond P.ProfessorPaulVezinaPaul Vezina41.78927490000000-87.601250000000001492Vezina, PaulPROFESSORWeiWeiwGdgHIF1GuTQkntjbwjr93A=Wei Wei41.78927490000000-87.601250000000001677Wei, WeiProfessorBenjaminGlickBenjamin Glick41.78927490000000-87.601250000000001957Glick, BenjaminProfessorGhanashyamGhadgeGhanashyam Ghadge41.78927490000000-87.601250000000002070Ghadge, GhanashyamSenior Research AssociateXiaoxiZhuangz3hhHqAbHszMknJtY0fp/Cv/+94=Xiaoxi Zhuang41.78927490000000-87.60125000000000285Zhuang, XiaoxiProfessor6.282250.00233538400research areas2.784410.021561716coauthor of49.16078.6947260similar to1182selected publicationsCliftonRagsdaleClifton Ragsdale41.78927490000000-87.60125000000000354Ragsdale, CliftonProfessorElizabethGroveElizabeth Grove41.78927490000000-87.60125000000000473Grove, ElizabethProfessorSangram S.SisodiaxHF6ArIaHeX5hHlsaUXv9Gq0+s+oSangram S. Sisodia41.78927490000000-87.60125000000000510Sisodia, Sangram S.ProfessorYusukeNakamuraYusuke Nakamura41.78927490000000-87.60125000000000582Nakamura, YusukeEmeritus/Emerita, ProfessorAnithaGovindAnitha Govind41.78927490000000-87.60125000000000795Govind, AnithaAuthorship 167779937884512Cao Y, Liu H, Lu SS, Jones KA, Govind AP, Jeyifous O, Simmons CQ, Tabatabaei N, Green WN, Holder JL, Tahmasebi S, George AL, Dickinson BCNature communicationsRNA-based translation activators for targeted gene upregulation. Nat Commun. 2023 10 26; 14(1):6827.Nat Commun2023-10-26T00:00:002023RNA-based translation activators for targeted gene upregulation.Daniel S.Mc Gehee029qDKQdHOn5lXtnYwj78G3z/Mq65vtbdGw=Daniel S. Mc Gehee41.78927490000000-87.60125000000000956Mc Gehee, Daniel S.ProfessorAuthorship 1682731137958495Zammit M, Kao CM, Zhang HJ, Tsai HM, Holderman N, Mitchell S, Tanios E, Bhuiyan M, Freifelder R, Kucharski A, Green WN, Mukherjee J, Chen CTInternational journal of molecular sciencesEvaluation of an Image-Derived Input Function for Kinetic Modeling of Nicotinic Acetylcholine Receptor-Binding PET Ligands in Mice. Int J Mol Sci. 2023 Oct 24; 24(21).Int J Mol Sci2023-10-24T00:00:002023Evaluation of an Image-Derived Input Function for Kinetic Modeling of Nicotinic Acetylcholine Receptor-Binding PET Ligands in Mice.http://weiw@uchicago.eduEmail: weiw@uchicago.edutrue1true1ProfessorProfessortrue1Emeritus/Emerita, ProfessorEmeritus/Emerita, Professortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1Senior Research AssociateSenior Research Associatetrue1ProfessorProfessortrue1PROFESSORPROFESSORAuthorship 752126Authorship 76752421081055Simonson PD, Deberg HA, Ge P, Alexander JK, Jeyifous O, Green WN, Selvin PRBiophysical journalCounting bungarotoxin binding sites of nicotinic acetylcholine receptors in mammalian cells with high signal/noise ratios. Biophys J. 2010 Nov 17; 99(10):L81-3.Biophys J2010-11-17T00:00:002010Counting bungarotoxin binding sites of nicotinic acetylcholine receptors in mammalian cells with high signal/noise ratios.25255882Cai E, Ge P, Lee SH, Jeyifous O, Wang Y, Liu Y, Wilson KM, Lim SJ, Baird MA, Stone JE, Lee KY, Davidson MW, Chung HJ, Schulten K, Smith AM, Green WN, Selvin PRAngewandte Chemie (International ed. in English)Stable small quantum dots for synaptic receptor tracking on live neurons. Angew Chem Int Ed Engl. 2014 Nov 10; 53(46):12484-8.Angew Chem Int Ed Engl2014-09-26T00:00:002014Stable small quantum dots for synaptic receptor tracking on live neurons.19620977Jeyifous O, Waites CL, Specht CG, Fujisawa S, Schubert M, Lin EI, Marshall J, Aoki C, de Silva T, Montgomery JM, Garner CC, Green WNNature neuroscienceSAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway. Nat Neurosci. 2009 Aug; 12(8):1011-9.Nat Neurosci2009-07-20T00:00:002009SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway.19357261Waites CL, Specht CG, Härtel K, Leal-Ortiz S, Genoux D, Li D, Drisdel RC, Jeyifous O, Cheyne JE, Green WN, Montgomery JM, Garner CCThe Journal of neuroscience : the official journal of the Society for NeuroscienceSynaptic SAP97 isoforms regulate AMPA receptor dynamics and access to presynaptic glutamate. J Neurosci. 2009 Apr 08; 29(14):4332-45.J Neurosci2009-04-08T00:00:002009Synaptic SAP97 isoforms regulate AMPA receptor dynamics and access to presynaptic glutamate.24454378Cortes CJ, Qin K, Norstrom EM, Green WN, Bindokas VP, Mastrianni JAInternational journal of cell biologyEarly Delivery of Misfolded PrP from ER to Lysosomes by Autophagy. Int J Cell Biol. 2013; 2013:560421.Int J Cell Biol2013-12-17T00:00:002013Early Delivery of Misfolded PrP from ER to Lysosomes by Autophagy.Authorship 79894525970033Zheng N, Jeyifous O, Munro C, Montgomery JM, Green WNeLifeSynaptic activity regulates AMPA receptor trafficking through different recycling pathways. Elife. 2015 May 13; 4.Elife2015-05-13T00:00:002015Synaptic activity regulates AMPA receptor trafficking through different recycling pathways.Psychiatry & Behav. NeurosciChicago60637ILAuthorship 612189Authorship 62862323331514Baker LK, Mao D, Chi H, Govind AP, Vallejo YF, Iacoviello M, Herrera S, Cortright JJ, Green WN, McGehee DS, Vezina PThe European journal of neuroscienceIntermittent nicotine exposure upregulates nAChRs in VTA dopamine neurons and sensitises locomotor responding to the drug. Eur J Neurosci. 2013 Mar; 37(6):1004-11.Eur J Neurosci2013-01-21T00:00:002013Intermittent nicotine exposure upregulates nAChRs in VTA dopamine neurons and sensitises locomotor responding to the drug.23864692Lin EI, Jeyifous O, Green WNThe Journal of neuroscience : the official journal of the Society for NeuroscienceCASK regulates SAP97 conformation and its interactions with AMPA and NMDA receptors. J Neurosci. 2013 Jul 17; 33(29):12067-76.J Neurosci2013-07-17T00:00:002013CASK regulates SAP97 conformation and its interactions with AMPA and NMDA receptors.23760509Antinone SE, Ghadge GD, Lam TT, Wang L, Roos RP, Green WNThe Journal of biological chemistryPalmitoylation of superoxide dismutase 1 (SOD1) is increased for familial amyotrophic lateral sclerosis-linked SOD1 mutants. J Biol Chem. 2013 Jul 26; 288(30):21606-17.J Biol Chem2013-06-12T00:00:002013Palmitoylation of superoxide dismutase 1 (SOD1) is increased for familial amyotrophic lateral sclerosis-linked SOD1 mutants.Authorship 98344827956638Jeyifous O, Lin EI, Chen X, Antinone SE, Mastro R, Drisdel R, Reese TS, Green WNProceedings of the National Academy of Sciences of the United States of AmericaPalmitoylation regulates glutamate receptor distributions in postsynaptic densities through control of PSD95 conformation and orientation. Proc Natl Acad Sci U S A. 2016 12 27; 113(52):E8482-E8491.Proc Natl Acad Sci U S A2016-12-12T00:00:002016Palmitoylation regulates glutamate receptor distributions in postsynaptic densities through control of PSD95 conformation and orientation.Authorship 99614528120938Antinone SE, Ghadge GD, Ostrow LW, Roos RP, Green WNScientific reportsS-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects. Sci Rep. 2017 01 25; 7:41141.Sci Rep2017-01-25T00:00:002017S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.