Robert J.KeenanRobert J. Keenan41.78927490000000-87.60125000000000401Keenan, Robert J.Membrane protein biogenesisMembrane protein quality controlStructural biologyBiochemical reconstitutionCell biologyWe study the molecular mechanisms underlying membrane protein biogenesis and quality control using biochemical, structural and cell biology approaches.Professorplugins:TwitterTwitterprns:coAuthorOfcoauthor ofprns:emailEncryptedemail addressprns:endDateend dateprns:fullNamefull nameprns:hasAuthorListauthor listprns:hasNetworkhas networkprns:hasPublicationVenuepublished inprns:informationResourceReferenceinformation resource referenceprns:isPrimaryPositionis primary positionprns:latitudelatitudeprns:longitudelongitudeprns:mainImagephotoprns:maxWeightmaximum weightprns:medlineTAjournal title abbreviationprns:meshDescriptorUIMeSH DescriptorUIprns:meshSemanticGroupNameMeSH semantic group nameprns:minWeightminimum weightprns:numberOfAuthorsnumber of authorsprns:numberOfConnectionsnumber of connectionsprns:numberOfPublicationsnumber of publicationsprns:personIdPerson IDprns:personInPrimaryPositionperson in primary positionprns:physicalNeighborOfphysical neighborprns:pluginSearchableDataProfilesRNS Plugin Searchable Dataprns:positionInDepartmentposition in departmentprns:predicateNodepredicate nodeprns:publicationDatepublication dateprns:similarTosimilar toprns:sortOrdersort orderprns:trainingAtOrganizationeducational organizationprns:trainingLocationtraining locationprns:uniquenessWeightuniqueness weightprns:yearyearAcademic ArticleArticleDocumentbibo:pmidPubMed IdentifierAddressvivo:addressCitycityvivo:addressPostalCodepostal codevivo:addressStatestate or provincevivo:authorInAuthorshipselected publicationsvivo:authorRankauthor rank in publicationAuthorshipvivo:degreeEarneddegree earnedDepartmentvivo:educationalTrainingeducation and trainingEducational Trainingvivo:freetextKeywordkeywordsvivo:hasResearchArearesearch areasvivo:hrJobTitleHR job titleInformation Resourcevivo:linkAnchorTextlink anchor textvivo:linkedAuthorlinked authorvivo:linkedInformationResourcelinked information resourcevivo:mailingAddressmailing addressvivo:majorFieldmajor field of degreevivo:overviewoverviewvivo:personInPositionpositionsPositionvivo:positionInOrganizationposition in organizationvivo:preferredTitlepreferred titleURLLinkvivo:webpagewebpagerdf:predicatepredicaterdf:typetyperdfs:labellabelConceptAgentfoaf:firstNamefirst namefoaf:lastNamelast nameOrganizationPerson06/1990Bates CollegeLewiston, MEBates College, Biology and ChemistryBSBiology and Chemistry05/1998UCSFSan Francisco, CAUCSF, Biochemistry and BiophysicsPHDBiochemistry and Biophysics09/1999UCSFSan Francisco, CAUCSF, Biochemistry and BiophysicsPostdocBiochemistry and BiophysicsAuthorship 1071025Authorship 1071033Authorship 1071044Authorship 107105388972771Schmitz U, Freymann DM, James TL, Keenan RJ, Vinayak R, Walter PRNA (New York, N.Y.)NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP). RNA. 1996 Dec; 2(12):1213-27.RNA1996-12-01T00:00:001996NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP).28712723Wohlever ML, Mateja A, McGilvray PT, Day KJ, Keenan RJMolecular cellMsp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins. Mol Cell. 2017 Jul 20; 67(2):194-202.e6.Mol Cell2017-07-14T00:00:002017Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins.8340363Rutenber E, Fauman EB, Keenan RJ, Fong S, Furth PS, Ortiz de Montellano PR, Meng E, Kuntz ID, DeCamp DL, Salto RThe Journal of biological chemistryStructure of a non-peptide inhibitor complexed with HIV-1 protease. Developing a cycle of structure-based drug design. J Biol Chem. 1993 Jul 25; 268(21):15343-6.J Biol Chem1993-07-25T00:00:001993Structure of a non-peptide inhibitor complexed with HIV-1 protease. Developing a cycle of structure-based drug design.D004721Anatomy1032500.867008Endoplasmic ReticulumBiochemistry and Molecular BiologyMolecular Genetics and Cell BiologyNeurobiologyAuthorship 1125512Authorship 1113265Authorship 112248430173121Mateja A, Keenan RJCurrent opinion in structural biologyA structural perspective on tail-anchored protein biogenesis by the GET pathway. Curr Opin Struct Biol. 2018 08; 51:195-202.Curr Opin Struct Biol2018-08-30T00:00:002018A structural perspective on tail-anchored protein biogenesis by the GET pathway.29281821Anghel SA, McGilvray PT, Hegde RS, Keenan RJCell reportsIdentification of Oxa1 Homologs Operating in the Eukaryotic Endoplasmic Reticulum. Cell Rep. 2017 12 26; 21(13):3708-3716.Cell Rep2017-12-26T00:00:002017Identification of Oxa1 Homologs Operating in the Eukaryotic Endoplasmic Reticulum.28877464Zalisko BE, Chan C, Denic V, Rock RS, Keenan RJCell reportsTail-Anchored Protein Insertion by a Single Get1/2 Heterodimer. Cell Rep. 2017 Sep 05; 20(10):2287-2293.Cell Rep2017-09-05T00:00:002017Tail-Anchored Protein Insertion by a Single Get1/2 Heterodimer.Authorship 162662D008565Chemicals & Drugs37111940.607165Membrane ProteinsAuthorship 149971Authorship 278324Authorship 272851Authorship 333568Authorship 344792Authorship 313944Authorship 414895Authorship 468252Authorship 364437Authorship 388355Authorship 388917Authorship 420736D012270Anatomy451110.945524RibosomesAuthorship 432332Authorship 137729732459176O'Donnell JP, Phillips BP, Yagita Y, Juszkiewicz S, Wagner A, Malinverni D, Keenan RJ, Miller EA, Hegde RSeLifeThe architecture of EMC reveals a path for membrane protein insertion. Elife. 2020 05 27; 9.Elife2020-05-27T00:00:002020The architecture of EMC reveals a path for membrane protein insertion.Authorship 451935D014176Physiology1533780.834518Protein BiosynthesisAuthorship 130822Authorship 140139932820719McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJeLifeAn ER translocon for multi-pass membrane protein biogenesis. Elife. 2020 08 21; 9.Elife2020-08-21T00:00:002020An ER translocon for multi-pass membrane protein biogenesis.Authorship 263847Authorship 377744Authorship 4596769002524Freymann DM, Keenan RJ, Stroud RM, Walter PNatureStructure of the conserved GTPase domain of the signal recognition particle. Nature. 1997 Jan 23; 385(6614):361-4.Nature1997-01-23T00:00:001997Structure of the conserved GTPase domain of the signal recognition particle.9695947Keenan RJ, Freymann DM, Walter P, Stroud RMCellCrystal structure of the signal sequence binding subunit of the signal recognition particle. Cell. 1998 Jul 24; 94(2):181-91.Cell1998-07-24T00:00:001998Crystal structure of the signal sequence binding subunit of the signal recognition particle.10426959Freymann DM, Keenan RJ, Stroud RM, Walter PNature structural biologyFunctional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP. Nat Struct Biol. 1999 Aug; 6(8):793-801.Nat Struct Biol1999-08-01T00:00:001999Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP.15668959Siehl DL, Castle LA, Gorton R, Chen YH, Bertain S, Cho HJ, Keenan R, Liu D, Lassner MWPest management scienceEvolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy. Pest Manag Sci. 2005 Mar; 61(3):235-40.Pest Manag Sci2005-03-01T00:00:002005Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy.16148303Yuan L, Kurek I, English J, Keenan RMicrobiology and molecular biology reviews : MMBRLaboratory-directed protein evolution. Microbiol Mol Biol Rev. 2005 Sep; 69(3):373-92.Microbiol Mol Biol Rev2005-09-01T00:00:002005Laboratory-directed protein evolution.17962222Strongin DE, Bevis B, Khuong N, Downing ME, Strack RL, Sundaram K, Glick BS, Keenan RJProtein engineering, design & selection : PEDSStructural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants. Protein Eng Des Sel. 2007 Nov; 20(11):525-34.Protein Eng Des Sel2007-10-25T00:00:002007Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.18953349Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BSNature methodsA noncytotoxic DsRed variant for whole-cell labeling. Nat Methods. 2008 Nov; 5(11):955-7.Nat Methods2008-10-26T00:00:002008A noncytotoxic DsRed variant for whole-cell labeling.19344508Strack RL, Bhattacharyya D, Glick BS, Keenan RJBMC biotechnologyNoncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling. BMC Biotechnol. 2009 Apr 03; 9:32.BMC Biotechnol2009-04-03T00:00:002009Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.19658435Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BSBiochemistryA rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Biochemistry. 2009 Sep 08; 48(35):8279-81.Biochemistry2009-09-08T00:00:002009A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.20509651Strack RL, Strongin DE, Mets L, Glick BS, Keenan RJJournal of the American Chemical SocietyChromophore formation in DsRed occurs by a branched pathway. J Am Chem Soc. 2010 Jun 23; 132(24):8496-505.J Am Chem Soc2010-06-23T00:00:002010Chromophore formation in DsRed occurs by a branched pathway.20676083Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan RJ, Hegde RSNatureA ribosome-associating factor chaperones tail-anchored membrane proteins. Nature. 2010 Aug 26; 466(7310):1120-4.Nature2010-08-01T00:00:002010A ribosome-associating factor chaperones tail-anchored membrane proteins.18330643Schnitzler CE, Keenan RJ, McCord R, Matysik A, Christianson LM, Haddock SHMarine biotechnology (New York, N.Y.)Spectral diversity of fluorescent proteins from the anthozoan Corynactis californica. Mar Biotechnol (NY). 2008 May-Jun; 10(3):328-42.Mar Biotechnol (NY)2008-03-11T00:00:002008Spectral diversity of fluorescent proteins from the anthozoan Corynactis californica.22086371Hegde RS, Keenan RJNature reviews. Molecular cell biologyTail-anchored membrane protein insertion into the endoplasmic reticulum. Nat Rev Mol Cell Biol. 2011 Nov 16; 12(12):787-98.Nat Rev Mol Cell Biol2011-11-16T00:00:002011Tail-anchored membrane protein insertion into the endoplasmic reticulum.21658170Sherrill J, Mariappan M, Dominik P, Hegde RS, Keenan RJTraffic (Copenhagen, Denmark)A conserved archaeal pathway for tail-anchored membrane protein insertion. Traffic. 2011 Sep; 12(9):1119-23.Traffic2011-07-03T00:00:002011A conserved archaeal pathway for tail-anchored membrane protein insertion.21116992Strack RL, Keenan RJ, Glick BSMethods in molecular biology (Clifton, N.J.)Noncytotoxic DsRed derivatives for whole-cell labeling. Methods Mol Biol. 2011; 699:355-70.Methods Mol Biol2011-01-01T00:00:002011Noncytotoxic DsRed derivatives for whole-cell labeling.Authorship 149935234556847Hegde RS, Keenan RJNature reviews. Molecular cell biologyThe mechanisms of integral membrane protein biogenesis. Nat Rev Mol Cell Biol. 2022 02; 23(2):107-124.Nat Rev Mol Cell Biol2021-09-23T00:00:002021The mechanisms of integral membrane protein biogenesis.15951425Keenan RJ, Siehl DL, Gorton R, Castle LAProceedings of the National Academy of Sciences of the United States of AmericaDNA shuffling as a tool for protein crystallization. Proc Natl Acad Sci U S A. 2005 Jun 21; 102(25):8887-92.Proc Natl Acad Sci U S A2005-06-10T00:00:002005DNA shuffling as a tool for protein crystallization.17272278Siehl DL, Castle LA, Gorton R, Keenan RJThe Journal of biological chemistryThe molecular basis of glyphosate resistance by an optimized microbial acetyltransferase. J Biol Chem. 2007 Apr 13; 282(15):11446-55.J Biol Chem2007-02-01T00:00:002007The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase.19675567Mateja A, Szlachcic A, Downing ME, Dobosz M, Mariappan M, Hegde RS, Keenan RJNatureThe structural basis of tail-anchored membrane protein recognition by Get3. Nature. 2009 Sep 17; 461(7262):361-6.Nature2009-08-12T00:00:002009The structural basis of tail-anchored membrane protein recognition by Get3.21866104Mariappan M, Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJNatureThe mechanism of membrane-associated steps in tail-anchored protein insertion. Nature. 2011 Aug 24; 477(7362):61-6.Nature2011-08-24T00:00:002011The mechanism of membrane-associated steps in tail-anchored protein insertion.D029701Chemicals & Drugs872760.797652Saccharomyces cerevisiae ProteinsAuthorship 1575494Authorship 157550636261528Smalinskaite L, Kim MK, Lewis AJO, Keenan RJ, Hegde RSNatureMechanism of an intramembrane chaperone for multipass membrane proteins. Nature. 2022 11; 611(7934):161-166.Nature2022-10-19T00:00:002022Mechanism of an intramembrane chaperone for multipass membrane proteins.36261522Sundaram A, Yamsek M, Zhong F, Hooda Y, Hegde RS, Keenan RJNatureSubstrate-driven assembly of a translocon for multipass membrane proteins. Nature. 2022 11; 611(7934):167-172.Nature2022-10-19T00:00:002022Substrate-driven assembly of a translocon for multipass membrane proteins.ChuanHe1Gp8Cq8dHMzMknJtY0fp/Cv/+94=Chuan He0.000000000000000.000000000000003123He, ChuanProfessorhttp://drummondlab.orgDrummond Labhttps://gibraltar.uchicago.eduRock Labhttp://keenanlab.bsd.uchicago.eduKeenan Labhttp://nerve.bsd.uchicago.eduExcitability, Molecular Basishttps://openwetware.org/wiki/Pan_LabPan Lab sitehttp://voices.uchicago.edu/drsisodialab/Sisodia Labhttp://he-group.uchicago.eduMy Lab Sitehttp://sosnick.uchicago.edu/Sosnick lab websitehttp://kosslab.uchicago.eduKosslabhttps://voices.uchicago.edu/phoebericelab/Rice Lab web sitehttp://glicklab.bsd.uchicago.edu/Glick Labhttp://nerve.bsd.uchicago.edu/FBLab Sitehttp://recombinant-antibodies.orgRecombinant Antibody NetworkdallandrummondChemistryUniversity of ChicagoAnthonyKossiakoff1mlmGLIcGOf5hHlsaUXv9Gq0+s+oAnthony Kossiakoff41.78927490000000-87.601250000000001188Kossiakoff, AnthonyProfessorTaoPanw2NmG6AbOfnamXNnYUHhvWD+6g==Tao Pan41.78927490000000-87.601250000000001308Pan, TaoProfessorJoseph A.Piccirilli3XJgCKIcC+X5hHlsaUXv9Gq0+s+oJoseph A. Piccirilli41.78927490000000-87.60125000000000190Piccirilli, Joseph A.ProfessorBenjaminGlickBenjamin Glick41.78927490000000-87.601250000000001957Glick, BenjaminProfessorPhoebe A.RicePhoebe A. Rice41.78927490000000-87.601250000000002362Rice, Phoebe A.ProfessorEduardoPerozo0nJsGa4PFszMknJtY0fp/Cv/+94=Eduardo Perozo41.78927490000000-87.601250000000002512Perozo, EduardoProfessor4.314360.0068811190research areas0.5416680.035295610coauthor of46.62645.5628960similar to1142selected publicationsSangram S.SisodiaxHF6ArIaHeX5hHlsaUXv9Gq0+s+oSangram S. Sisodia41.78927490000000-87.60125000000000510Sisodia, Sangram S.ProfessorRonald S.RockRonald S. Rock41.78927490000000-87.60125000000000548Rock, Ronald S.Associate ProfessorTobin R.Sosnickw3B6BLIbEO/5hHlsaUXv9Gq0+s+oTobin R. Sosnick41.78927490000000-87.60125000000000744Sosnick, Tobin R.ProfessorHsin-YangChangHsin-Yang Chang41.78927490000000-87.60125000000000775Chang, Hsin-YangFranciscoBezanillaFrancisco Bezanilla41.78927490000000-87.60125000000000987Bezanilla, FranciscoProfessorD. AllanDrummondD. Allan Drummond41.78927490000000-87.60125000000000990Drummond, D. AllanAssociate Professortrue1true1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1Associate ProfessorAssociate Professortrue1Associate ProfessorAssociate Professortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessortrue1ProfessorProfessorAuthorship 732177Authorship 72298825392301Bestul AJ, Christensen JR, Grzegorzewska AP, Burke TA, Sees JA, Carroll RT, Sirotkin V, Keenan RJ, Kovar DRMolecular biology of the cellFission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12. Mol Biol Cell. 2015 Jan 15; 26(2):283-93.Mol Biol Cell2014-11-12T00:00:002014Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.25745174Mateja A, Paduch M, Chang HY, Szydlowska A, Kossiakoff AA, Hegde RS, Keenan RJScience (New York, N.Y.)Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 2015 Mar 06; 347(6226):1152-5.Science2015-03-06T00:00:002015Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.Authorship 82597526256539Borowska MT, Dominik PK, Anghel SA, Kossiakoff AA, Keenan RJStructure (London, England : 1993)A YidC-like Protein in the Archaeal Plasma Membrane. Structure. 2015 Sep 01; 23(9):1715-1724.Structure2015-08-06T00:00:002015A YidC-like Protein in the Archaeal Plasma Membrane.Chicago60637ILAuthorship 871356Authorship 87325826729921Gomes AC, Kordala AJ, Strack R, Wang X, Geslain R, Delaney K, Clark WC, Keenan R, Pan TRNA (New York, N.Y.)A dual fluorescent reporter for the investigation of methionine mistranslation in live cells. RNA. 2016 Mar; 22(3):467-76.RNA2016-01-04T00:00:002016A dual fluorescent reporter for the investigation of methionine mistranslation in live cells.26749445Dominik PK, Borowska MT, Dalmas O, Kim SS, Perozo E, Keenan RJ, Kossiakoff AAStructure (London, England : 1993)Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs. Structure. 2016 Feb 02; 24(2):300-9.Structure2015-12-31T00:00:002015Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.26947396Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Fromme JC, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz PNature communicationsData publication with the structural biology data grid supports live analysis. Nat Commun. 2016 Mar 07; 7:10882.Nat Commun2016-03-07T00:00:002016Data publication with the structural biology data grid supports live analysis.Authorship 91544527345149Itakura E, Zavodszky E, Shao S, Wohlever ML, Keenan RJ, Hegde RSMolecular cellUbiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation. Mol Cell. 2016 07 07; 63(1):21-33.Mol Cell2016-06-23T00:00:002016Ubiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation.Authorship 93617410580470Schmitz U, Behrens S, Freymann DM, Keenan RJ, Lukavsky P, Walter P, James TLRNA (New York, N.Y.)Structure of the phylogenetically most conserved domain of SRP RNA. RNA. 1999 Nov; 5(11):1419-29.RNA1999-11-01T00:00:001999Structure of the phylogenetically most conserved domain of SRP RNA.Authorship 536972Authorship 543381Authorship 54088111395422Keenan RJ, Freymann DM, Stroud RM, Walter PAnnual review of biochemistryThe signal recognition particle. Annu Rev Biochem. 2001; 70:755-75.Annu Rev Biochem2001-01-01T00:00:002001The signal recognition particle.11875408Keenan RJ, Stemmer WPNature biotechnologyNontransgenic crops from transgenic plants. Nat Biotechnol. 2002 Mar; 20(3):215-6.Nat Biotechnol2002-03-01T00:00:002002Nontransgenic crops from transgenic plants.10712156Walter P, Keenan R, Schmitz UScience (New York, N.Y.)Perspectives: structural biology. SRP--where the RNA and membrane worlds meet. Science. 2000 Feb 18; 287(5456):1212-3.Science2000-02-18T00:00:002000Perspectives: structural biology. SRP--where the RNA and membrane worlds meet.Authorship 97352327923985Srivastava R, Zalisko BE, Keenan RJ, Howell SHPlant physiologyThe GET System Inserts the Tail-Anchored Protein, SYP72, into Endoplasmic Reticulum Membranes. Plant Physiol. 2017 02; 173(2):1137-1145.Plant Physiol2016-12-06T00:00:002016The GET System Inserts the Tail-Anchored Protein, SYP72, into Endoplasmic Reticulum Membranes.