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One or more keywords matched the following properties of Kossiakoff, Anthony
keywords Protein Engineering
keywords T-cell Engineering
keywords Antibody Engineering
overview The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.
One or more keywords matched the following items that are connected to Kossiakoff, Anthony
Item TypeName
Concept Antibodies, Monoclonal
Concept Antibodies, Blocking
Concept Antibodies, Bispecific
Concept Antibodies, Monoclonal, Humanized
Concept Single-Chain Antibodies
Concept Antibodies, Neutralizing
Concept Antibodies
Academic Article Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution.
Academic Article Synthetic antibodies for specific recognition and crystallization of structured RNA.
Academic Article The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Academic Article Exploring and designing protein function with restricted diversity.
Academic Article Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Academic Article X-ray structures of the antigen-binding domains from three variants of humanized anti-p185HER2 antibody 4D5 and comparison with molecular modeling.
Academic Article Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Academic Article Molecular recognition in biological systems: from activation to inhibition.
Academic Article High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Academic Article An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Academic Article Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Academic Article Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies.
Academic Article Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Academic Article Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Academic Article Scalable high throughput selection from phage-displayed synthetic antibody libraries.
Academic Article Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Academic Article Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Academic Article A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
Academic Article Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.
Academic Article A polar ring endows improved specificity to an antibody fragment.
Academic Article Generating Conformation and Complex-Specific Synthetic Antibodies.
Academic Article Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody.
Academic Article Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Academic Article Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination.
Academic Article Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Academic Article An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Academic Article Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Award or Honor Receipt Director Department of Protein Engineering
Academic Article Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Academic Article Engineered Ultra-High Affinity Synthetic Antibodies for SARS-CoV-2 Neutralization and Detection.
Academic Article Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on av Integrins.
Academic Article A T cell redirection platform for co-targeting dual antigens on solid tumors.
Academic Article Structure of an AMPK complex in an inactive, ATP-bound state.
Academic Article Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.
Academic Article Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels.
Academic Article Synthetic Antibodies Detect Distinct Cellular States of Chromosome Passenger Complex Proteins.
Academic Article Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
Concept Single-Domain Antibodies
Grant Chaperone Assisted Crystallography
Grant Recombinant Antibody Network
Grant Energetics of cytokine-induced receptor signaling
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  • Antibody
  • Engineering