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Search Results to Anthony Kossiakoff

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One or more keywords matched the following properties of Kossiakoff, Anthony

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keywords Phage Display Mutagenesis
overview The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

One or more keywords matched the following items that are connected to Kossiakoff, Anthony

Item TypeName
Concept Bacteriophages
Concept Mutagenesis
Concept Mutagenesis, Site-Directed
Concept Bacteriophage P22
Concept Bacteriophage M13
Academic Article Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
Academic Article Crystallization and preliminary X-ray analysis of a 1:1 complex between a designed monomeric interferon-gamma and its soluble receptor.
Academic Article Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article High affinity RNase S-peptide variants obtained by phage display have a novel "hot-spot" of binding energy.
Academic Article The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species.
Academic Article Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Academic Article The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.
Academic Article Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
Academic Article Dissecting the binding energy epitope of a high-affinity variant of human growth hormone: cooperative and additive effects from combining mutations from independently selected phage display mutagenesis libraries.
Academic Article The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled.
Academic Article Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Academic Article Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Academic Article Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution.
Academic Article The crystal structure of affinity-matured human growth hormone at 2 A resolution.
Academic Article Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Academic Article Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis.
Academic Article High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Academic Article Scalable high throughput selection from phage-displayed synthetic antibody libraries.
Academic Article Phage display selections for affinity reagents to membrane proteins in nanodiscs.
Academic Article Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.
Academic Article Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.

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  • Phage
  • Display
  • Mutagenesis