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Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution.
The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Molecular recognition in biological systems: from activation to inhibition.
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
A polar ring endows improved specificity to an antibody fragment.
Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination.
An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
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Director Department of Protein Engineering
Structure of an AMPK complex in an inactive, ATP-bound state.
Energetics of cytokine-induced receptor signaling