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One or more keywords matched the following properties of Kossiakoff, Anthony
keywords T-cell Engineering
overview The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.
One or more keywords matched the following items that are connected to Kossiakoff, Anthony
Item TypeName
Concept Adenosine Triphosphatases
Concept Adenosine Triphosphate
Concept Bacterial Toxins
Concept Biological Transport
Concept Guanosine Triphosphate
Concept Genetic Complementation Test
Concept Nerve Tissue Proteins
Concept Hot Temperature
Concept Tissue Plasminogen Activator
Concept Protein Processing, Post-Translational
Concept Transcription Factors
Concept Trypsin Inhibitors
Concept Salmonella typhimurium
Concept Time Factors
Concept Thermodynamics
Concept Tryptophan
Concept Trypsinogen
Concept Protein Structure, Tertiary
Concept Neutralization Tests
Concept Protein Transport
Concept Combinatorial Chemistry Techniques
Concept Focal Adhesion Protein-Tyrosine Kinases
Concept High-Throughput Screening Assays
Concept Trypsin
Concept Adaptor Proteins, Signal Transducing
Concept Temperature
Concept Tetrahymena
Concept Trypsin Inhibitor, Kunitz Soybean
Concept RNA Transport
Concept Cell Line, Tumor
Concept Tissue Array Analysis
Concept T-Lymphocytes
Concept Thromboplastin
Concept Signal Transduction
Concept Helix-Turn-Helix Motifs
Concept Membrane Transport Proteins
Academic Article Variability of conformations at crystal contacts in BPTI represent true low-energy structures: correspondence among lattice packing and molecular dynamics structures.
Academic Article Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
Academic Article Hydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures.
Academic Article Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article Human growth hormone and extracellular domain of its receptor: crystal structure of the complex.
Academic Article Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor.
Academic Article Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.
Academic Article High affinity RNase S-peptide variants obtained by phage display have a novel "hot-spot" of binding energy.
Academic Article Solvent structure in crystals of trypsin determined by X-ray and neutron diffraction.
Academic Article Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Academic Article The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex.
Academic Article Deciphering the role of the electrostatic interactions involving Gly70 in eglin C by total chemical protein synthesis.
Academic Article Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.
Academic Article Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.
Academic Article Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 A.
Academic Article Neutron structure of subtilisin BPN': effects of chemical environment on hydrogen-bonding geometries and the pattern of hydrogen-deuterium exchange in secondary structure elements.
Academic Article Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
Academic Article Structure of bistramide A-actin complex at a 1.35 angstroms resolution.
Academic Article Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Academic Article Crystal structure and site 1 binding energetics of human placental lactogen.
Academic Article Synthetic antibodies for specific recognition and crystallization of structured RNA.
Academic Article The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.
Academic Article Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.
Academic Article Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Academic Article The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Academic Article Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Academic Article Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Academic Article Hydroxyl hydrogen conformations in trypsin determined by the neutron diffraction solvent difference map method: relative importance of steric and electrostatic factors in defining hydrogen-bonding geometries.
Academic Article Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Academic Article Tertiary structure is a principal determinant to protein deamidation.
Academic Article Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Academic Article Structure of bovine trypsinogen at 1.9 A resolution.
Academic Article X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.
Academic Article Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution.
Academic Article The X-ray structure of a growth hormone-prolactin receptor complex.
Academic Article Crystal structure of full-length KcsA in its closed conformation.
Academic Article A method of solvent structure analysis for proteins using D2O-H2O neutron difference maps.
Academic Article Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin.
Academic Article Neutron diffraction identifies His 57 as the catalytic base in trypsin.
Academic Article X-ray structures of the antigen-binding domains from three variants of humanized anti-p185HER2 antibody 4D5 and comparison with molecular modeling.
Academic Article Automated peak fitting procedure for processing data from an area detector and its application to the neutron structure of trypsin.
Academic Article Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Academic Article Structure of the growth hormone-receptor complex and mechanism of receptor signaling.
Academic Article Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors.
Academic Article Molecular recognition in biological systems: from activation to inhibition.
Academic Article High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.
Academic Article Mechanisms of zymogen activation.
Academic Article Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Academic Article The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.
Academic Article Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Academic Article Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.
Academic Article An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Academic Article The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Academic Article The application of neutron crystallography to the study of dynamic and and hydration properties of proteins.
Academic Article Protein dynamics investigated by the neutron diffraction-hydrogen exchange technique.
Academic Article A comparison of neutron diffraction and molecular dynamics structures: hydroxyl group and water molecule orientations in trypsin.
Academic Article Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Academic Article Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Academic Article Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Academic Article Scalable high throughput selection from phage-displayed synthetic antibody libraries.
Academic Article Nuclear pores. Architecture of the nuclear pore complex coat.
Academic Article Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.
Academic Article Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Academic Article Cell biology. New tricks for an old dimer.
Academic Article Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Academic Article A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.
Academic Article A YidC-like Protein in the Archaeal Plasma Membrane.
Academic Article A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
Academic Article Architecture of the fungal nuclear pore inner ring complex.
Academic Article Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.
Academic Article A polar ring endows improved specificity to an antibody fragment.
Academic Article The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical.
Academic Article Prolactin Receptor-Mediated Internalization of Imaging Agents Detects Epithelial Ovarian Cancer with Enhanced Sensitivity and Specificity.
Academic Article Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody.
Academic Article Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Academic Article Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article Reply to Kang and Brooks: Comment on the calculations in protein thermodynamics.
Academic Article Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Academic Article The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab.
Academic Article Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Academic Article Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Academic Article An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Academic Article Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Academic Article Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Academic Article Structure and mechanism of the ER-based glucosyltransferase ALG6.
Academic Article Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Academic Article Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.
Academic Article Engineered Ultra-High Affinity Synthetic Antibodies for SARS-CoV-2 Neutralization and Detection.
Academic Article Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on av Integrins.
Academic Article Structural basis of omega-3 fatty acid transport across the blood-brain barrier.
Academic Article A T cell redirection platform for co-targeting dual antigens on solid tumors.
Academic Article K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.
Academic Article Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Academic Article Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Academic Article Structure of an AMPK complex in an inactive, ATP-bound state.
Academic Article Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers.
Academic Article Architecture of the cytoplasmic face of the nuclear pore.
Academic Article Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver.
Academic Article A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
Academic Article Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias.
Academic Article Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase.
Academic Article Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
Concept Cell Surface Display Techniques
Concept In Vitro Techniques
Grant Chaperone Assisted Crystallography
Grant Chaperone-Enabled studies of epigenetic regulation enzymes
Grant Recombinant Antibody Network
Grant Energetics of cytokine-induced receptor signaling
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  • cell Engineering