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Kossiakoff, Anthony

One or more keywords matched the following properties of Kossiakoff, Anthony

Property	Value
keywords	T-cell Engineering
overview	The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

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Value

keywords

T-cell Engineering

overview

The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

One or more keywords matched the following items that are connected to Kossiakoff, Anthony

Item Type	Name
Concept	Adenosine Triphosphatases
Concept	Adenosine Triphosphate
Concept	Bacterial Toxins
Concept	Biological Transport
Concept	Guanosine Triphosphate
Concept	Genetic Complementation Test
Concept	Nerve Tissue Proteins
Concept	Hot Temperature
Concept	Tissue Plasminogen Activator
Concept	Protein Processing, Post-Translational
Concept	Transcription Factors
Concept	Trypsin Inhibitors
Concept	Salmonella typhimurium
Concept	Time Factors
Concept	Thermodynamics
Concept	Tryptophan
Concept	Trypsinogen
Concept	Protein Structure, Tertiary
Concept	Neutralization Tests
Concept	Protein Transport
Concept	Combinatorial Chemistry Techniques
Concept	Focal Adhesion Protein-Tyrosine Kinases
Concept	High-Throughput Screening Assays
Concept	Trypsin
Concept	Adaptor Proteins, Signal Transducing
Concept	Temperature
Concept	Tetrahymena
Concept	Trypsin Inhibitor, Kunitz Soybean
Concept	Cation Transport Proteins
Concept	RNA Transport
Concept	Cell Line, Tumor
Concept	Tissue Array Analysis
Concept	T-Lymphocytes
Concept	Thromboplastin
Concept	Signal Transduction
Concept	Helix-Turn-Helix Motifs
Concept	Membrane Transport Proteins
Academic Article	Variability of conformations at crystal contacts in BPTI represent true low-energy structures: correspondence among lattice packing and molecular dynamics structures.
Academic Article	Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
Academic Article	Hydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures.
Academic Article	Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article	Human growth hormone and extracellular domain of its receptor: crystal structure of the complex.
Academic Article	Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor.
Academic Article	Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article	Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.
Academic Article	High affinity RNase S-peptide variants obtained by phage display have a novel "hot-spot" of binding energy.
Academic Article	Solvent structure in crystals of trypsin determined by X-ray and neutron diffraction.
Academic Article	Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Academic Article	The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex.
Academic Article	Deciphering the role of the electrostatic interactions involving Gly70 in eglin C by total chemical protein synthesis.
Academic Article	Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.
Academic Article	Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.
Academic Article	Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 A.
Academic Article	Neutron structure of subtilisin BPN': effects of chemical environment on hydrogen-bonding geometries and the pattern of hydrogen-deuterium exchange in secondary structure elements.
Academic Article	Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
Academic Article	Structure of bistramide A-actin complex at a 1.35 angstroms resolution.
Academic Article	Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Academic Article	Crystal structure and site 1 binding energetics of human placental lactogen.
Academic Article	Synthetic antibodies for specific recognition and crystallization of structured RNA.
Academic Article	The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.
Academic Article	Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.
Academic Article	Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Academic Article	The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Academic Article	Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Academic Article	Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Academic Article	Hydroxyl hydrogen conformations in trypsin determined by the neutron diffraction solvent difference map method: relative importance of steric and electrostatic factors in defining hydrogen-bonding geometries.
Academic Article	Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Academic Article	Tertiary structure is a principal determinant to protein deamidation.
Academic Article	Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Academic Article	Structure of bovine trypsinogen at 1.9 A resolution.
Academic Article	X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.
Academic Article	Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution.
Academic Article	The X-ray structure of a growth hormone-prolactin receptor complex.
Academic Article	Crystal structure of full-length KcsA in its closed conformation.
Academic Article	A method of solvent structure analysis for proteins using D2O-H2O neutron difference maps.
Academic Article	Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin.
Academic Article	Neutron diffraction identifies His 57 as the catalytic base in trypsin.
Academic Article	X-ray structures of the antigen-binding domains from three variants of humanized anti-p185HER2 antibody 4D5 and comparison with molecular modeling.
Academic Article	Automated peak fitting procedure for processing data from an area detector and its application to the neutron structure of trypsin.
Academic Article	Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Academic Article	Structure of the growth hormone-receptor complex and mechanism of receptor signaling.
Academic Article	Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors.
Academic Article	Molecular recognition in biological systems: from activation to inhibition.
Academic Article	High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article	Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.
Academic Article	Mechanisms of zymogen activation.
Academic Article	Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Academic Article	The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.
Academic Article	Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Academic Article	Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.
Academic Article	An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Academic Article	The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Academic Article	The application of neutron crystallography to the study of dynamic and and hydration properties of proteins.
Academic Article	Protein dynamics investigated by the neutron diffraction-hydrogen exchange technique.
Academic Article	A comparison of neutron diffraction and molecular dynamics structures: hydroxyl group and water molecule orientations in trypsin.
Academic Article	Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Academic Article	Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Academic Article	Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Academic Article	Scalable high throughput selection from phage-displayed synthetic antibody libraries.
Academic Article	Nuclear pores. Architecture of the nuclear pore complex coat.
Academic Article	Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.
Academic Article	Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Academic Article	Cell biology. New tricks for an old dimer.
Academic Article	Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Academic Article	A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.
Academic Article	A YidC-like Protein in the Archaeal Plasma Membrane.
Academic Article	A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
Academic Article	Architecture of the fungal nuclear pore inner ring complex.
Academic Article	Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.
Academic Article	A polar ring endows improved specificity to an antibody fragment.
Academic Article	The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical.
Academic Article	Prolactin Receptor-Mediated Internalization of Imaging Agents Detects Epithelial Ovarian Cancer with Enhanced Sensitivity and Specificity.
Academic Article	Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody.
Academic Article	Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Academic Article	Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article	Reply to Kang and Brooks: Comment on the calculations in protein thermodynamics.
Academic Article	Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Academic Article	The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab.
Academic Article	Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Academic Article	Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Academic Article	An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Academic Article	Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Academic Article	Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Academic Article	Structure and mechanism of the ER-based glucosyltransferase ALG6.
Academic Article	Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Academic Article	Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.
Academic Article	Engineered Ultra-High Affinity Synthetic Antibodies for SARS-CoV-2 Neutralization and Detection.
Academic Article	Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on av Integrins.
Academic Article	Structural basis of omega-3 fatty acid transport across the blood-brain barrier.
Academic Article	A T cell redirection platform for co-targeting dual antigens on solid tumors.
Academic Article	K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.
Academic Article	Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Academic Article	Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Academic Article	Structure of an AMPK complex in an inactive, ATP-bound state.
Academic Article	Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers.
Academic Article	Architecture of the cytoplasmic face of the nuclear pore.
Academic Article	Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver.
Academic Article	A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
Academic Article	Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias.
Academic Article	Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase.
Academic Article	Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
Concept	Solute Carrier Organic Anion Transporter Family Member 1B3
Concept	Cell Surface Display Techniques
Concept	In Vitro Techniques
Grant	Chaperone Assisted Crystallography
Grant	Chaperone-Enabled studies of epigenetic regulation enzymes
Grant	Recombinant Antibody Network
Grant	Energetics of cytokine-induced receptor signaling
Academic Article	Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum.
Academic Article	Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA.
Academic Article	Structure of human drug transporters OATP1B1 and OATP1B3.
Academic Article	Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains.

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T
cell Engineering