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Search Results to Anthony Kossiakoff

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One or more keywords matched the following properties of Kossiakoff, Anthony

keywords Membrane Proteins
keywords Protein Engineering
overview The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

One or more keywords matched the following items that are connected to Kossiakoff, Anthony

Item TypeName
Concept Bacterial Proteins
Concept Calmodulin-Binding Proteins
Concept Fungal Proteins
Concept Microfilament Proteins
Concept Nerve Tissue Proteins
Concept Protein Denaturation
Concept Plant Proteins
Concept Muscle Proteins
Concept Protein Methyltransferases
Concept Protein Precursors
Concept Protein Binding
Concept Protein Conformation
Concept Protein Processing, Post-Translational
Concept Proteins
Concept Protein Structure, Quaternary
Concept Protozoan Proteins
Concept Protein Structure, Tertiary
Concept Amyloid beta-Protein Precursor
Concept RNA-Binding Proteins
Concept GTP-Binding Protein alpha Subunits, Gs
Concept GTP-Binding Protein alpha Subunits, Gi-Go
Concept Protein Transport
Concept Protein Structure, Secondary
Concept Protein Folding
Concept Oncogene Proteins, Viral
Concept Receptors, G-Protein-Coupled
Concept Green Fluorescent Proteins
Concept G-Protein-Coupled Receptor Kinase 1
Concept Cell Cycle Proteins
Concept ras GTPase-Activating Proteins
Concept Nuclear Pore Complex Proteins
Concept Arabidopsis Proteins
Concept Saccharomyces cerevisiae Proteins
Concept Focal Adhesion Protein-Tyrosine Kinases
Concept Recombinant Proteins
Concept Adaptor Proteins, Signal Transducing
Concept Protein Interaction Domains and Motifs
Concept Serine Proteinase Inhibitors
Concept Protein Multimerization
Concept Viral Proteins
Concept GTP-Binding Proteins
Concept Antifreeze Proteins
Concept Structural Homology, Protein
Concept Mutant Proteins
Concept Escherichia coli Proteins
Concept Protein Stability
Concept Carrier Proteins
Concept Nuclear Proteins
Concept Recombinant Fusion Proteins
Concept Archaeal Proteins
Concept Membrane Transport Proteins
Concept Maltose-Binding Proteins
Academic Article Variability of conformations at crystal contacts in BPTI represent true low-energy structures: correspondence among lattice packing and molecular dynamics structures.
Academic Article Structural consequences of mutation.
Academic Article Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
Academic Article Hydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures.
Academic Article Crystallization and preliminary X-ray analysis of a 1:1 complex between a designed monomeric interferon-gamma and its soluble receptor.
Academic Article Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article Crystallization of ovine placental lactogen in a 1:2 complex with the extracellular domain of the rat prolactin receptor.
Academic Article Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor.
Academic Article X-ray structure of human relaxin at 1.5 A. Comparison to insulin and implications for receptor binding determinants.
Academic Article Probing intermolecular backbone H-bonding in serine proteinase-protein inhibitor complexes.
Academic Article Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.
Academic Article Structural basis for cytokine hormone-receptor recognition and receptor activation.
Academic Article Solvent structure in crystals of trypsin determined by X-ray and neutron diffraction.
Academic Article The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species.
Academic Article Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Academic Article The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex.
Academic Article Deciphering the role of the electrostatic interactions involving Gly70 in eglin C by total chemical protein synthesis.
Academic Article Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.
Academic Article Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.
Academic Article The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.
Academic Article Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 A.
Academic Article Crystals of the complex between human growth hormone and the extracellular domain of its receptor.
Academic Article Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution.
Academic Article Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
Academic Article The first semi-synthetic serine protease made by native chemical ligation.
Academic Article The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold.
Academic Article Structure of bistramide A-actin complex at a 1.35 angstroms resolution.
Academic Article Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Academic Article Crystal structure and site 1 binding energetics of human placental lactogen.
Academic Article X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers.
Academic Article The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.
Academic Article Dissecting the binding energy epitope of a high-affinity variant of human growth hormone: cooperative and additive effects from combining mutations from independently selected phage display mutagenesis libraries.
Academic Article The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled.
Academic Article Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.
Academic Article Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Academic Article X-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid beta-protein precursor.
Academic Article Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.
Academic Article The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Academic Article Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Academic Article Exploring and designing protein function with restricted diversity.
Academic Article Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Academic Article Mechanism of activation gating in the full-length KcsA K+ channel.
Academic Article Hydroxyl hydrogen conformations in trypsin determined by the neutron diffraction solvent difference map method: relative importance of steric and electrostatic factors in defining hydrogen-bonding geometries.
Academic Article Understanding mechanisms governing protein-protein interactions from synthetic binding interfaces.
Academic Article Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Academic Article Tertiary structure is a principal determinant to protein deamidation.
Academic Article Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Academic Article Structure of bovine trypsinogen at 1.9 A resolution.
Academic Article X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.
Academic Article Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution.
Academic Article The X-ray structure of a growth hormone-prolactin receptor complex.
Academic Article Crystal structure of full-length KcsA in its closed conformation.
Academic Article A method of solvent structure analysis for proteins using D2O-H2O neutron difference maps.
Academic Article Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin.
Academic Article X-ray structures of the antigen-binding domains from three variants of humanized anti-p185HER2 antibody 4D5 and comparison with molecular modeling.
Academic Article The crystal structure of affinity-matured human growth hormone at 2 A resolution.
Academic Article Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Academic Article Protein dynamics investigated by neutron diffraction.
Academic Article Automated peak fitting procedure for processing data from an area detector and its application to the neutron structure of trypsin.
Academic Article Neutron protein crystallography: advances in methods and applications.
Academic Article Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Academic Article Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors.
Academic Article Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis.
Academic Article Molecular recognition in biological systems: from activation to inhibition.
Academic Article High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article The production and X-ray structure determination of perdeuterated Staphylococcal nuclease.
Academic Article Effect of protein packing structure on side-chain methyl rotor conformations.
Academic Article Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods.
Academic Article The use of neutrons to show how proteins work.
Academic Article Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.
Academic Article Mechanisms of zymogen activation.
Academic Article Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Academic Article The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.
Academic Article The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).
Academic Article Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Academic Article Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.
Academic Article An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Academic Article Characterization of engineered actin binding proteins that control filament assembly and structure.
Academic Article The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Academic Article The application of neutron crystallography to the study of dynamic and and hydration properties of proteins.
Academic Article Protein dynamics investigated by the neutron diffraction-hydrogen exchange technique.
Academic Article Structure of active ß-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.
Academic Article Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Academic Article Visualization of arrestin recruitment by a G-protein-coupled receptor.
Academic Article Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies.
Academic Article Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Academic Article Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Academic Article Nuclear pores. Architecture of the nuclear pore complex coat.
Academic Article Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.
Academic Article Phage display selections for affinity reagents to membrane proteins in nanodiscs.
Academic Article Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Academic Article Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Academic Article Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Academic Article Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Academic Article A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.
Academic Article A YidC-like Protein in the Archaeal Plasma Membrane.
Academic Article A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
Academic Article Architecture of the fungal nuclear pore inner ring complex.
Academic Article Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.
Academic Article Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.
Academic Article A polar ring endows improved specificity to an antibody fragment.
Academic Article Specific Recognition of a Single-Stranded RNA Sequence by a Synthetic Antibody Fragment.
Academic Article The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical.
Academic Article Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Academic Article Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination.
Academic Article Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article Publisher Correction: Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article Reply to Kang and Brooks: Comment on the calculations in protein thermodynamics.
Academic Article Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Academic Article Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Academic Article The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab.
Academic Article Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Academic Article Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Academic Article An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Academic Article Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Academic Article Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Award or Honor Receipt Christian Anfinsen Award
Award or Honor Receipt Director Department of Protein Engineering
Academic Article Structure and mechanism of the ER-based glucosyltransferase ALG6.
Academic Article Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.

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