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Kossiakoff, Anthony

One or more keywords matched the following properties of Kossiakoff, Anthony

Property	Value
keywords	Membrane Proteins
keywords	Protein Engineering
overview	The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

One or more keywords matched the following items that are connected to Kossiakoff, Anthony

Item Type	Name
Concept	Bacterial Proteins
Concept	Calmodulin-Binding Proteins
Concept	Fungal Proteins
Concept	Chromosomal Proteins, Non-Histone
Concept	Microfilament Proteins
Concept	Nerve Tissue Proteins
Concept	Protein Denaturation
Concept	Plant Proteins
Concept	Muscle Proteins
Concept	Protein Methyltransferases
Concept	Protein Precursors
Concept	Protein Binding
Concept	Protein Conformation
Concept	Protein Processing, Post-Translational
Concept	Proteins
Concept	Protein Structure, Quaternary
Concept	Protozoan Proteins
Concept	Protein Structure, Tertiary
Concept	Ubiquitin-Protein Ligase Complexes
Concept	Amyloid beta-Protein Precursor
Concept	RNA-Binding Proteins
Concept	GTP-Binding Protein alpha Subunits, Gs
Concept	GTP-Binding Protein alpha Subunits, Gi-Go
Concept	Protein Transport
Concept	Protein Structure, Secondary
Concept	Protein Folding
Concept	Oncogene Proteins, Viral
Concept	Receptors, G-Protein-Coupled
Concept	Green Fluorescent Proteins
Concept	G-Protein-Coupled Receptor Kinase 1
Concept	Cell Cycle Proteins
Concept	ras GTPase-Activating Proteins
Concept	Nuclear Pore Complex Proteins
Concept	Arabidopsis Proteins
Concept	Saccharomyces cerevisiae Proteins
Concept	Focal Adhesion Protein-Tyrosine Kinases
Concept	Recombinant Proteins
Concept	Ubiquitin-Protein Ligases
Concept	Adaptor Proteins, Signal Transducing
Concept	Protein Interaction Domains and Motifs
Concept	Serine Proteinase Inhibitors
Concept	Protein Multimerization
Concept	Viral Proteins
Concept	GTP-Binding Proteins
Concept	Sequence Analysis, Protein
Concept	Antifreeze Proteins
Concept	Cation Transport Proteins
Concept	Structural Homology, Protein
Concept	Mutant Proteins
Concept	Escherichia coli Proteins
Concept	AMP-Activated Protein Kinases
Concept	Protein Stability
Concept	Carrier Proteins
Concept	Neoplasm Proteins
Concept	Nuclear Proteins
Concept	Recombinant Fusion Proteins
Concept	Archaeal Proteins
Concept	Protein Isoforms
Concept	Membrane Transport Proteins
Concept	Maltose-Binding Proteins
Academic Article	Variability of conformations at crystal contacts in BPTI represent true low-energy structures: correspondence among lattice packing and molecular dynamics structures.
Academic Article	Structural consequences of mutation.
Academic Article	Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
Academic Article	Hydroxyl and water molecule orientations in trypsin: comparison to molecular dynamic structures.
Academic Article	Crystallization and preliminary X-ray analysis of a 1:1 complex between a designed monomeric interferon-gamma and its soluble receptor.
Academic Article	Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities.
Academic Article	Crystallization of ovine placental lactogen in a 1:2 complex with the extracellular domain of the rat prolactin receptor.
Academic Article	Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor.
Academic Article	X-ray structure of human relaxin at 1.5 A. Comparison to insulin and implications for receptor binding determinants.
Academic Article	Probing intermolecular backbone H-bonding in serine proteinase-protein inhibitor complexes.
Academic Article	Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity.
Academic Article	Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.
Academic Article	Structural basis for cytokine hormone-receptor recognition and receptor activation.
Academic Article	Solvent structure in crystals of trypsin determined by X-ray and neutron diffraction.
Academic Article	The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species.
Academic Article	Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Academic Article	The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex.
Academic Article	Deciphering the role of the electrostatic interactions involving Gly70 in eglin C by total chemical protein synthesis.
Academic Article	Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.
Academic Article	Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.
Academic Article	The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.
Academic Article	Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 A.
Academic Article	Crystals of the complex between human growth hormone and the extracellular domain of its receptor.
Academic Article	Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution.
Academic Article	Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
Academic Article	The first semi-synthetic serine protease made by native chemical ligation.
Academic Article	The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold.
Academic Article	Structure of bistramide A-actin complex at a 1.35 angstroms resolution.
Academic Article	Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Academic Article	Crystal structure and site 1 binding energetics of human placental lactogen.
Academic Article	X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers.
Academic Article	The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.
Academic Article	Dissecting the binding energy epitope of a high-affinity variant of human growth hormone: cooperative and additive effects from combining mutations from independently selected phage display mutagenesis libraries.
Academic Article	The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled.
Academic Article	Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.
Academic Article	Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Academic Article	X-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid beta-protein precursor.
Academic Article	Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.
Academic Article	The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Academic Article	Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Academic Article	Exploring and designing protein function with restricted diversity.
Academic Article	Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Academic Article	Mechanism of activation gating in the full-length KcsA K+ channel.
Academic Article	Hydroxyl hydrogen conformations in trypsin determined by the neutron diffraction solvent difference map method: relative importance of steric and electrostatic factors in defining hydrogen-bonding geometries.
Academic Article	Understanding mechanisms governing protein-protein interactions from synthetic binding interfaces.
Academic Article	Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Academic Article	Tertiary structure is a principal determinant to protein deamidation.
Academic Article	Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Academic Article	Structure of bovine trypsinogen at 1.9 A resolution.
Academic Article	X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.
Academic Article	Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution.
Academic Article	The X-ray structure of a growth hormone-prolactin receptor complex.
Academic Article	Crystal structure of full-length KcsA in its closed conformation.
Academic Article	A method of solvent structure analysis for proteins using D2O-H2O neutron difference maps.
Academic Article	Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin.
Academic Article	X-ray structures of the antigen-binding domains from three variants of humanized anti-p185HER2 antibody 4D5 and comparison with molecular modeling.
Academic Article	The crystal structure of affinity-matured human growth hormone at 2 A resolution.
Academic Article	Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Academic Article	Protein dynamics investigated by neutron diffraction.
Academic Article	Automated peak fitting procedure for processing data from an area detector and its application to the neutron structure of trypsin.
Academic Article	Neutron protein crystallography: advances in methods and applications.
Academic Article	Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Academic Article	Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors.
Academic Article	Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis.
Academic Article	Molecular recognition in biological systems: from activation to inhibition.
Academic Article	High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Academic Article	The production and X-ray structure determination of perdeuterated Staphylococcal nuclease.
Academic Article	Effect of protein packing structure on side-chain methyl rotor conformations.
Academic Article	Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods.
Academic Article	The use of neutrons to show how proteins work.
Academic Article	Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.
Academic Article	Mechanisms of zymogen activation.
Academic Article	Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Academic Article	The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.
Academic Article	The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).
Academic Article	Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Academic Article	Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.
Academic Article	An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Academic Article	Characterization of engineered actin binding proteins that control filament assembly and structure.
Academic Article	The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Academic Article	The application of neutron crystallography to the study of dynamic and and hydration properties of proteins.
Academic Article	Protein dynamics investigated by the neutron diffraction-hydrogen exchange technique.
Academic Article	Structure of active ß-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.
Academic Article	Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Academic Article	Visualization of arrestin recruitment by a G-protein-coupled receptor.
Academic Article	Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies.
Academic Article	Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Academic Article	Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Academic Article	Nuclear pores. Architecture of the nuclear pore complex coat.
Academic Article	Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.
Academic Article	Phage display selections for affinity reagents to membrane proteins in nanodiscs.
Academic Article	Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Academic Article	Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Academic Article	Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Academic Article	Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Academic Article	A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.
Academic Article	A YidC-like Protein in the Archaeal Plasma Membrane.
Academic Article	A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
Academic Article	Architecture of the fungal nuclear pore inner ring complex.
Academic Article	Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.
Academic Article	Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.
Academic Article	A polar ring endows improved specificity to an antibody fragment.
Academic Article	Specific Recognition of a Single-Stranded RNA Sequence by a Synthetic Antibody Fragment.
Academic Article	The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical.
Academic Article	Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Academic Article	Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination.
Academic Article	Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article	Publisher Correction: Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Academic Article	Reply to Kang and Brooks: Comment on the calculations in protein thermodynamics.
Academic Article	Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Academic Article	Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Academic Article	The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab.
Academic Article	Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Academic Article	Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Academic Article	An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Academic Article	Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Academic Article	Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Award or Honor Receipt	Christian Anfinsen Award
Award or Honor Receipt	Director Department of Protein Engineering
Academic Article	Structure and mechanism of the ER-based glucosyltransferase ALG6.
Academic Article	Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Academic Article	Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.
Academic Article	Structural basis of omega-3 fatty acid transport across the blood-brain barrier.
Academic Article	Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Academic Article	Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Academic Article	Structure of an AMPK complex in an inactive, ATP-bound state.
Academic Article	Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.
Academic Article	Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers.
Academic Article	Structural basis of lipopolysaccharide maturation by the O-antigen ligase.
Academic Article	Synthetic Antibodies Detect Distinct Cellular States of Chromosome Passenger Complex Proteins.
Academic Article	Architecture of the cytoplasmic face of the nuclear pore.
Academic Article	A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
Academic Article	Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias.
Academic Article	Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase.
Academic Article	Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
Academic Article	Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder.
Concept	Protein Domains
Concept	Protein Conformation, alpha-Helical
Concept	Protein Conformation, beta-Strand
Grant	Chaperone Assisted Crystallography
Grant	Chaperone-Enabled studies of epigenetic regulation enzymes
Grant	Recombinant Antibody Network
Grant	Energetics of cytokine-induced receptor signaling
Academic Article	Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels.
Academic Article	Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum.
Academic Article	Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA.
Academic Article	Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry.
Academic Article	Structure of human drug transporters OATP1B1 and OATP1B3.
Academic Article	Engineered antigen-binding fragments for enhanced crystallization of antibody:antigen complexes.
Academic Article	Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding.
Academic Article	Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP.

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