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One or more keywords matched the following properties of Shuman, Howard A.
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keywords bacterial genetics
overview Our group is interested in how intracellular bacterial pathogens interact with and commandeer host cell functions during infection. We have studied the intracellular human pathogen, Legionella pneumophila for more than 30 years. This gram-negative species causes an acute febrile pneumonia called Legionnaires’ disease. People become infected with Legionella when they inhale contaminated aerosols. This can happen when cooling and ventilation systems, fountains, or whirlpools become contaminated. In these environments, Legionella replicates in single-celled amoebae. In human lungs these bacteria can infect and replicate in alveolar macrophages. In order to understand how Legionella is able to survive and grow inside amoebae and macrophages, we use genetic and genomic approaches to ask which functions in the bacteria and which ones in the host are important for infection. We found that Legionella has a “Type IV Secretion System” called the Icm/Dot System that translocates protein “effectors” to the host cell and that these effectors dramatically alter organelle trafficking in host cells as is shown in the accompanying diagram. The Legionella – containing vacuole, (LCV) is where the bacteria grow within cells in a nutrient-rich, protected niche. Current Position: 2010 - Professor of Microbiology, University of Chicago Education: 1970-1974 University of Massachusetts, B.S. (Microbiology, honors) 1975-1980 Harvard University, Ph.D. (Biological Chemistry) 1980-1982 Postdoctoral Fellow, Department of Biochemistry & Molecular Biology, Harvard University Employment / Experience: 1974-1975 Research Assistant, Institut Pasteur, Paris, France 1982-1989 Assistant Professor, Department of Microbiology, Columbia University 1989-1995 Associate Professor, Department of Microbiology, Columbia University 1995- 2010 Professor, Department of Microbiology, Columbia University 2005- 2008 Director of Graduate Studies, Department of Microbiology, Columbia University 2008 Interim Chair, Department of Microbiology, Columbia University 2010 Professor Emeritus, Department of Microbiology & Immunology, Columbia University 2010-2015 Director, Howard Taylor Ricketts Regional Biocontainment Laboratory Professional Service: 1986- 1997 Editorial Board, Journal of Bacteriology 1989- 1992 Editorial Board, Infection and Immunity 1994- 1999 Editorial Board, The Journal of Biological Chemistry 1998 Co-Chair, Gordon Research Conference: "Bacterial Cell Surfaces" 2007 Guest Editor, Proceedings of the National Academy of Sciences 2009 Editorial Board, Protist 2009 - 2010 Steering Committee, Northeast Biodefense Center 2009 - 2019 Editorial Board, mBio
One or more keywords matched the following items that are connected to Shuman, Howard A.
Item TypeName
Concept Antigens, Bacterial
Concept Bacterial Adhesion
Concept Bacterial Outer Membrane Proteins
Concept Bacterial Proteins
Concept Chromosomes, Bacterial
Concept DNA, Bacterial
Concept Genes, Bacterial
Concept RNA, Bacterial
Concept Transformation, Bacterial
Concept Genome, Bacterial
Concept Drug Resistance, Multiple, Bacterial
Concept Drug Resistance, Bacterial
Concept Anti-Bacterial Agents
Concept Gene Expression Regulation, Bacterial
Academic Article Genetic analysis of periplasmic binding protein dependent transport in Escherichia coli. Each lobe of maltose-binding protein interacts with a different subunit of the MalFGK2 membrane transport complex.
Academic Article Intergration of the receptor for bacteriophage lambda in the outer membrane of Escherichia coli: coupling with cell division.
Academic Article Interaction between maltose-binding protein and the membrane-associated maltose transporter complex in Escherichia coli.
Academic Article The inhibition of maltose transport by the unliganded form of the maltose-binding protein of Escherichia coli: experimental findings and mathematical treatment.
Academic Article Crystal structures and solution conformations of a dominant-negative mutant of Escherichia coli maltose-binding protein.
Academic Article Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages.
Academic Article Characterization of a new region required for macrophage killing by Legionella pneumophila.
Academic Article Genetics of Legionella pneumophila virulence.
Academic Article Unliganded maltose-binding protein triggers lactose transport in an Escherichia coli mutant with an alteration in the maltose transport system.
Academic Article Truncation of MalF results in lactose transport via the maltose transport system of Escherichia coli.
Academic Article Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila.
Academic Article Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages.
Academic Article Legionella pneumophila contains a type II general secretion pathway required for growth in amoebae as well as for secretion of the Msp protease.
Academic Article Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome.
Academic Article Possible origin of the Legionella pneumophila virulence genes and their relation to Coxiella burnetii.
Academic Article Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components of IncQ plasmid RSF1010.
Academic Article Mechanism of maltose transport in Escherichia coli: transmembrane signaling by periplasmic binding proteins.
Academic Article The detergent-soluble maltose transporter is activated by maltose binding protein and verapamil.
Academic Article The Legionella pneumophila icmGCDJBF genes are required for killing of human macrophages.
Academic Article The ATP-binding cassette subunit of the maltose transporter MalK antagonizes MalT, the activator of the Escherichia coli mal regulon.
Academic Article Icm/dot-dependent upregulation of phagocytosis by Legionella pneumophila.
Academic Article Disulfide cross-linking reveals a site of stable interaction between C-terminal regulatory domains of the two MalK subunits in the maltose transport complex.
Academic Article Virulence conversion of Legionella pneumophila by conjugal transfer of chromosomal DNA.
Academic Article The art and design of genetic screens: Escherichia coli.
Academic Article Comparative sequence analysis of the icm/dot genes in Legionella.
Academic Article Legionella effectors that promote nonlytic release from protozoa.
Academic Article The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase.
Academic Article The amoebae plate test implicates a paralogue of lpxB in the interaction of Legionella pneumophila with Acanthamoeba castellanii.
Academic Article Pathogen effector protein screening in yeast identifies Legionella factors that interfere with membrane trafficking.
Academic Article An immunoprotective molecule, the major secretory protein of Legionella pneumophila, is not a virulence factor in a guinea pig model of Legionnaires' disease.
Academic Article The genomic sequence of the accidental pathogen Legionella pneumophila.
Academic Article Legionella eukaryotic-like type IV substrates interfere with organelle trafficking.
Academic Article The Legionella pneumophila major secretory protein, a protease, is not required for intracellular growth or cell killing.
Academic Article ArgR-regulated genes are derepressed in the Legionella-containing vacuole.
Academic Article Cyclic diguanylate signaling proteins control intracellular growth of Legionella pneumophila.
Academic Article Host cell-dependent secretion and translocation of the LepA and LepB effectors of Legionella pneumophila.
Academic Article Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon.
Academic Article Loss of RNase R induces competence development in Legionella pneumophila.
Academic Article Antibiotics and UV radiation induce competence for natural transformation in Legionella pneumophila.
Academic Article Use of gene fusions to study outer membrane protein localization in Escherichia coli.
Academic Article The overlapping host responses to bacterial cyclic dinucleotides.
Academic Article The activities of the Escherichia coli MalK protein in maltose transport, regulation, and inducer exclusion can be separated by mutations.
Academic Article The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophages.
Academic Article Evidence for acquisition of Legionella type IV secretion substrates via interdomain horizontal gene transfer.
Academic Article The perplexing functions and surprising origins of Legionella pneumophila type IV secretion effectors.
Academic Article Chemical genetics reveals bacterial and host cell functions critical for type IV effector translocation by Legionella pneumophila.
Academic Article Intracellular bacteria encode inhibitory SNARE-like proteins.
Academic Article The Legionella pneumophila effector VipA is an actin nucleator that alters host cell organelle trafficking.
Academic Article Chemical synthesis enables biochemical and antibacterial evaluation of streptolydigin antibiotics.
Academic Article Whole-genome sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H.
Academic Article Genetics of Legionella pneumophila.
Academic Article Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system.
Academic Article The genetics of active transport in bacteria.
Academic Article Escherichia coli F plasmid transfers to and replicates within Legionella pneumophila: an alternative to using an RP4-based system for gene delivery.
Academic Article Transport of p-nitrophenyl-alpha-maltoside by the maltose transport system of Escherichia coli and its subsequent hydrolysis by a cytoplasmic alpha-maltosidase.
Academic Article The iron superoxide dismutase of Legionella pneumophila is essential for viability.
Academic Article Genetics and molecular pathogenesis of Legionella pneumophila, an intracellular parasite of macrophages.
Academic Article Genetic evidence for substrate and periplasmic-binding-protein recognition by the MalF and MalG proteins, cytoplasmic membrane components of the Escherichia coli maltose transport system.
Academic Article Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli.
Academic Article Identification of the malK gene product. A peripheral membrane component of the Escherichia coli maltose transport system.
Academic Article The maltose-maltodextrin transport system of Escherichia coli.
Academic Article Tinkering with transporters: periplasmic binding protein-dependent maltose transport in E. coli.
Academic Article Identification of Legionella pneumophila genes required for growth within and killing of human macrophages.
Academic Article Mathematical treatment of the kinetics of binding protein dependent transport systems reveals that both the substrate loaded and unloaded binding proteins interact with the membrane components.
Academic Article Characterization of the structural requirements for assembly and nucleotide binding of an ATP-binding cassette transporter. The maltose transport system of Escherichia coli.
Academic Article Mutagenesis of Legionella pneumophila using Tn903 dlllacZ: identification of a growth-phase-regulated pigmentation gene.
Academic Article The use of gene fusions of study bacterial transport proteins.
Academic Article Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.
Academic Article Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.
Academic Article Mutations that alter the transmembrane signalling pathway in an ATP binding cassette (ABC) transporter.
Academic Article Maltose transport in Escherichia coli: mutations that uncouple ATP hydrolysis from transport.
Academic Article The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii.
Academic Article The HL-60 model for the interaction of human macrophages with the Legionnaires' disease bacterium.
Academic Article Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils.
Academic Article SigmaS controls multiple pathways associated with intracellular multiplication of Legionella pneumophila.
Academic Article Legionella pneumophila 6S RNA optimizes intracellular multiplication.
Academic Article Methods to study legionella transcriptome in vitro and in vivo.
Academic Article Overproduction of MalK protein prevents expression of the Escherichia coli mal regulon.
Academic Article Allele-specific malE mutations that restore interactions between maltose-binding protein and the inner-membrane components of the maltose transport system.
Academic Article The effect of nalidixic acid on the expression of some genes in Escherichia coli K-12.
Academic Article Transposition of bacteriophage Mu in the Legionnaires disease bacterium.
Academic Article Amphipols: polymeric surfactants for membrane biology research.
Academic Article Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.
Academic Article Legionella pneumophila transcriptional response following exposure to CuO nanoparticles.
Academic Article Computational modeling and experimental validation of the Legionella and Coxiella virulence-related type-IVB secretion signal.
Academic Article A bacterial protein promotes the recognition of the Legionella pneumophila vacuole by autophagy.
Academic Article Host-directed antimicrobial drugs with broad-spectrum efficacy against intracellular bacterial pathogens.
Academic Article Genetic Manipulation of Acinetobacter baumannii.
Academic Article Analysis of the transcriptome of Legionella pneumophila hfq mutant reveals a new mobile genetic element.
Academic Article Identification of novel Coxiella burnetii Icm/Dot effectors and genetic analysis of their involvement in modulating a mitogen-activated protein kinase pathway.
Academic Article Joint Transcriptional Control of Virulence and Resistance to Antibiotic and Environmental Stress in Acinetobacter baumannii.
Academic Article Genomic analysis of 38 Legionella species identifies large and diverse effector repertoires.
Academic Article Direct targeting of membrane fusion by SNARE mimicry: Convergent evolution of Legionella effectors.
Academic Article Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria.
Academic Article GigA and GigB are Master Regulators of Antibiotic Resistance, Stress Responses, and Virulence in Acinetobacter baumannii.
Academic Article Gallium disrupts bacterial iron metabolism and has therapeutic effects in mice and humans with lung infections.
Academic Article Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms.
Academic Article GigC, a LysR Family Transcription Regulator, Is Required for Cysteine Metabolism and Virulence in Acinetobacter baumannii.
Academic Article Acinetobacter baumannii ATCC 17978 encodes a microcin system with antimicrobial properties for contact-independent competition.
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