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Search Results to Benjamin Glick

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One or more keywords matched the following properties of Glick, Benjamin

keywords Microscopy, Fluorescence
keywords Microscopy, Electron
overview Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.

One or more keywords matched the following items that are connected to Glick, Benjamin

Item TypeName
Concept Microscopy, Immunoelectron
Concept Microscopy, Video
Concept Microscopy, Confocal
Concept Microscopy, Fluorescence, Multiphoton
Academic Article Isolation of highly purified mitochondria from Saccharomyces cerevisiae.
Academic Article Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.
Academic Article Raising the speed limits for 4D fluorescence microscopy.
Academic Article Monitoring changes in the subcellular location of proteins in S. cerevisiae.
Academic Article The transitional ER localization mechanism of Pichia pastoris Sec12.
Academic Article Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed).
Academic Article De novo formation of transitional ER sites and Golgi structures in Pichia pastoris.
Academic Article Sec16 is a determinant of transitional ER organization.
Academic Article Golgi maturation visualized in living yeast.
Academic Article Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance.
Academic Article Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.
Academic Article A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.
Academic Article Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
Academic Article Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
Academic Article Fluorescence microscopy and thin-section electron microscopy.
Academic Article Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
Academic Article Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Academic Article Imaging pancreatic beta-cells in the intact pancreas.
Academic Article Sec16 influences transitional ER sites by regulating rather than organizing COPII.
Academic Article COPI selectively drives maturation of the early Golgi.
Academic Article Visualizing Secretory Cargo Transport in Budding Yeast.
Academic Article Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Academic Article 4D Microscopy of Yeast.
Academic Article A microscopy-based kinetic analysis of yeast vacuolar protein sorting.

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