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Search Results to Dominique Missiakas

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One or more keywords matched the following properties of Missiakas, Dominique

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keywords Protein secretion
overview Dr. Missiakas’s research program examines the Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis. This program seeks to appreciate the biogenesis of the envelope of these microorganisms as well as the secretion of their virulence factors and host pathways targeted by these pathogens. Pathogenesis and vaccine studies in S. aureus: Many molecules in the envelope of these bacteria affect their interactions with the host. In staphylococci, cell wall anchored proteins contribute to pathogen survival in blood, dissemination to tissues, formation of abscesses and persistence in host tissues as well as subversion of immune functions. The systematic analysis of mutants lacking all or anyone of twenty cell wall anchored proteins has proven a very powerful approach in identifying protective antigens for vaccine design and elucidating virulence pathways. S. aureus is the only bacterial pathogen known to coagulate plasma and to agglutinate with fibrin cables in blood. A molecular examination of agglutination revealed that this process requires the secreted products of the coa (coagulase), vwb (von Willebrand binding protein), and clfA (Clumping factor A) genes. We are examining a model whereby S. aureus agglutination involves the formation of staphylothrombin (Coa, vWbp)-assembled fibrin cables, capped by clumping factor A (ClfA) on the staphylococcal surface and crosslinked by factor XIII. In this model, S. aureus agglutination provides for escape from phagocytic killing and bacterial traffic across the vasculature. In this manner, the Coa-vWbp-ClfA mediated agglutination pathway promotes the formation of abscess lesions, where S. aureus replicates as a fibrin encapsulated bacterial community, protected from immune cells. This key virulence strategy can be perturbed with monoclonal antibodies and small molecule inhibitors to either prevent or treat S. aureus sepsis Cell envelope assembly: Bacteria assemble polymers in their envelope that are important for their viability as well as their interaction with hosts. Our research program combines bacterial genetics, physiology and biochemistry as well as small molecule chemical tools approaches to characterize the complex biochemical pathways that enable envelope assembly in Gram-positive microbes. Potential innovations derived from this work are the identification of key enzymes for the biosynthesis of lipoteichoic acid (LTA), wall teichoic acid (WTA), secondary cell wall polysaccharide (SCWP) and capsular polymer (CPS, PDGA). In Bacilli, the SCWP is also involved in the retention of proteins that bear the S-layer homology domain (SLH proteins). SLH proteins play important function for cell separation, sporulation as well as virulence. Protein secretion: One aspect of this program examines a Sec-independent secretion system named ESAT-6 like Secretion System (ESS). Biochemical and genetic approaches are used to isolate and characterize the ESS translocon, identify secreted substrates and elucidate their function during infection of hosts.

One or more keywords matched the following items that are connected to Missiakas, Dominique

Item TypeName
Concept Bacterial Outer Membrane Proteins
Concept Bacterial Proteins
Concept Complement System Proteins
Concept Fungal Proteins
Concept DNA-Binding Proteins
Concept Membrane Proteins
Concept Protein Denaturation
Concept Monosaccharide Transport Proteins
Concept Heat-Shock Proteins
Concept Hemolysin Proteins
Concept Opsonin Proteins
Concept Protein Binding
Concept Protein Conformation
Concept Proteins
Concept Protein Disulfide Reductase (Glutathione)
Concept Staphylococcal Protein A
Concept Protein Engineering
Concept Protein Structure, Quaternary
Concept Protein Structure, Tertiary
Concept Receptor Protein-Tyrosine Kinases
Concept Protein Transport
Concept Multidrug Resistance-Associated Proteins
Concept Protein Structure, Secondary
Concept Protein Folding
Concept Periplasmic Binding Proteins
Concept Protein Disulfide-Isomerases
Concept Recombinant Proteins
Concept Periplasmic Proteins
Concept Protein Interaction Domains and Motifs
Concept Protein Multimerization
Concept Protein-Serine-Threonine Kinases
Concept Cation Transport Proteins
Concept Protein Sorting Signals
Concept Escherichia coli Proteins
Concept Fimbriae Proteins
Concept Pore Forming Cytotoxic Proteins
Concept Calcium-Binding Proteins
Concept Carrier Proteins
Concept Luminescent Proteins
Concept Recombinant Fusion Proteins
Concept Membrane Transport Proteins
Concept Protein Array Analysis
Concept Protein Kinase Inhibitors
Concept Maltose-Binding Proteins
Academic Article The Escherichia coli heat shock gene htpY: mutational analysis, cloning, sequencing, and transcriptional regulation.
Academic Article Making and breaking disulfide bonds.
Academic Article New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH.
Academic Article Effects of mutations in genes for proteins involved in disulphide bond formation in the periplasm on the activities of anaerobically induced electron transfer chains in Escherichia coli K12.
Academic Article Protein folding in the bacterial periplasm.
Academic Article Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli.
Academic Article Protein misfolding in the cell envelope of Escherichia coli: new signaling pathways.
Academic Article Probing the structural role of an alpha beta loop of maltose-binding protein by mutagenesis: heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies.
Academic Article DsbD-catalyzed transport of electrons across the membrane of Escherichia coli.
Academic Article Transfer of electrons across the cytoplasmic membrane by DsbD, a membrane protein involved in thiol-disulphide exchange and protein folding in the bacterial periplasm.
Academic Article Characterization of the Escherichia coli sigma E regulon.
Academic Article The slow-refolding step of phosphoglycerate kinase as monitored by pulse proteolysis.
Academic Article RseB binding to the periplasmic domain of RseA modulates the RseA:sigmaE interaction in the cytoplasm and the availability of sigmaE.RNA polymerase.
Academic Article Thiol-disulfide exchange in an immunoglobulin-like fold: structure of the N-terminal domain of DsbD.
Academic Article Passage of heme-iron across the envelope of Staphylococcus aureus.
Academic Article Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site.
Academic Article EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections.
Academic Article The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions.
Academic Article Activation of inhibitors by sortase triggers irreversible modification of the active site.
Academic Article Unfolding-refolding of the domains in yeast phosphoglycerate kinase: comparison with the isolated engineered domains.
Academic Article Changes in lipopolysaccharide structure induce the sigma(E)-dependent response of Escherichia coli.
Academic Article EsaC substrate for the ESAT-6 secretion pathway and its role in persistent infections of Staphylococcus aureus.
Academic Article ESAT-6-like protein secretion in Bacillus anthracis.
Academic Article Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL.
Academic Article Flexibility and folding of phosphoglycerate kinase.
Academic Article Nontoxigenic protein A vaccine for methicillin-resistant Staphylococcus aureus infections in mice.
Academic Article EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.
Academic Article Identifying protective antigens of Staphylococcus aureus, a pathogen that suppresses host immune responses.
Academic Article Staphylococcus aureus secretes coagulase and von Willebrand factor binding protein to modify the coagulation cascade and establish host infections.
Academic Article Protein secretion and surface display in Gram-positive bacteria.
Academic Article Secretion genes as determinants of Bacillus anthracis chain length.
Academic Article Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood.
Academic Article Efficient expression and characterization of isolated structural domains of yeast phosphoglycerate kinase generated by site-directed mutagenesis.
Academic Article Characterization of EssB, a protein required for secretion of ESAT-6 like proteins in Staphylococcus aureus.
Academic Article Survey of the folding pathway of a two-domain protein phosphoglycerate kinase.
Academic Article Golden pigment production and virulence gene expression are affected by metabolisms in Staphylococcus aureus.
Academic Article A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus.
Academic Article Sortase-conjugation generates a capsule vaccine that protects guinea pigs against Bacillus anthracis.
Academic Article A play in four acts: Staphylococcus aureus abscess formation.
Academic Article Protein A-specific monoclonal antibodies and prevention of Staphylococcus aureus disease in mice.
Academic Article Expression of multidrug resistance efflux pump gene norA is iron responsive in Staphylococcus aureus.
Academic Article Nuclear magnetic resonance studies of isolated structural domains of yeast phosphoglycerate kinase.
Academic Article LytN, a murein hydrolase in the cross-wall compartment of Staphylococcus aureus, is involved in proper bacterial growth and envelope assembly.
Academic Article Structural and functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli.
Academic Article Coagulases as determinants of protective immune responses against Staphylococcus aureus.
Academic Article Kinetic studies of the refolding of yeast phosphoglycerate kinase: comparison with the isolated engineered domains.
Academic Article The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation.
Academic Article Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli.
Academic Article Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus.
Academic Article The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli.
Academic Article Crystal structure of Bacillus anthracis transpeptidase enzyme CapD.
Academic Article Contribution of coagulases towards Staphylococcus aureus disease and protective immunity.
Academic Article The SLH-domain protein BslO is a determinant of Bacillus anthracis chain length.
Academic Article Surface-layer (S-layer) proteins sap and EA1 govern the binding of the S-layer-associated protein BslO at the cell septa of Bacillus anthracis.
Academic Article Abscess formation and alpha-hemolysin induced toxicity in a mouse model of Staphylococcus aureus peritoneal infection.
Academic Article Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB.
Academic Article Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins.
Academic Article Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons.
Academic Article The functional properties of DsbG, a thiol-disulfide oxidoreductase from the periplasm of Escherichia coli.
Academic Article Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor.
Academic Article Staphylococcus aureus synthesizes adenosine to escape host immune responses.
Academic Article IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge.
Academic Article ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus.
Academic Article Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Academic Article Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo.
Academic Article Bacillus anthracis acetyltransferases PatA1 and PatA2 modify the secondary cell wall polysaccharide and affect the assembly of S-layer proteins.
Academic Article Multiple ligands of von Willebrand factor-binding protein (vWbp) promote Staphylococcus aureus clot formation in human plasma.
Academic Article LytR-CpsA-Psr enzymes as determinants of Bacillus anthracis secondary cell wall polysaccharide assembly.
Academic Article Protein A-neutralizing monoclonal antibody protects neonatal mice against Staphylococcus aureus.
Academic Article Role of protein A in the evasion of host adaptive immune responses by Staphylococcus aureus.
Academic Article Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs.
Academic Article A monoclonal antibody that recognizes the E domain of staphylococcal protein A.
Academic Article Secretion of atypical protein substrates by the ESAT-6 secretion system of Staphylococcus aureus.
Academic Article Glutamate Racemase Mutants of Bacillus anthracis.
Academic Article Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria.
Academic Article N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus bloodstream infection.
Academic Article Release of protein A from the cell wall of Staphylococcus aureus.
Academic Article The capsular polysaccharide of Staphylococcus aureus is attached to peptidoglycan by the LytR-CpsA-Psr (LCP) family of enzymes.
Academic Article GneZ, a UDP-GlcNAc 2-epimerase, is required for S-layer assembly and vegetative growth of Bacillus anthracis.
Academic Article The giant protein Ebh is a determinant of Staphylococcus aureus cell size and complement resistance.
Academic Article Adhesion of Staphylococcus aureus to the vessel wall under flow is mediated by von Willebrand factor-binding protein.
Academic Article EsxB, a secreted protein from Bacillus anthracis forms two distinct helical bundles.
Academic Article Bacillus anthracis SlaQ Promotes S-Layer Protein Assembly.
Academic Article Bacillus anthracis tagO Is Required for Vegetative Growth and Secondary Cell Wall Polysaccharide Synthesis.
Academic Article Bacillus anthracis lcp Genes Support Vegetative Growth, Envelope Assembly, and Spore Formation.
Academic Article SagB Glucosaminidase Is a Determinant of Staphylococcus aureus Glycan Chain Length, Antibiotic Susceptibility, and Protein Secretion.
Academic Article Antibodies against a secreted product of Staphylococcus aureus trigger phagocytic killing.
Academic Article Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion during Staphylococcus aureus infection.
Academic Article Contribution of Staphylococcus aureus Coagulases and Clumping Factor A to Abscess Formation in a Rabbit Model of Skin and Soft Tissue Infection.
Academic Article Genes Required for Bacillus anthracis Secondary Cell Wall Polysaccharide Synthesis.
Academic Article EssE Promotes Staphylococcus aureus ESS-Dependent Protein Secretion To Modify Host Immune Responses during Infection.
Academic Article EssD, a Nuclease Effector of the Staphylococcus aureus ESS Pathway.
Academic Article Clumping factor A, von Willebrand factor-binding protein and von Willebrand factor anchor Staphylococcus aureus to the vessel wall.
Academic Article Assembly and Function of the Bacillus anthracis S-Layer.
Academic Article A novel STK1-targeted small-molecule as an "antibiotic resistance breaker" against multidrug-resistant Staphylococcus aureus.
Academic Article Galactosylation of the Secondary Cell Wall Polysaccharide of Bacillus anthracis and Its Contribution to Anthrax Pathogenesis.
Academic Article EssH Peptidoglycan Hydrolase Enables Staphylococcus aureus Type VII Secretion across the Bacterial Cell Wall Envelope.
Academic Article Isolation of a Membrane Protein Complex for Type VII Secretion in Staphylococcus aureus.
Academic Article The transmembrane domain of the Staphylococcus aureus ESAT-6 component EssB mediates interaction with the integral membrane protein EsaA, facilitating partially regulated secretion in a heterologous host.
Academic Article Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.
Academic Article Staphylococcal Protein A Contributes to Persistent Colonization of Mice with Staphylococcus aureus.
Academic Article Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis.
Academic Article Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A.
Academic Article Sortases, Surface Proteins, and Their Roles in Staphylococcus aureus Disease and Vaccine Development.
Academic Article Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion.
Academic Article Staphylococcal Protein Secretion and Envelope Assembly.
Academic Article FPR1 is the plague receptor on host immune cells.
Academic Article Distinct Pathways Carry Out a and ß Galactosylation of Secondary Cell Wall Polysaccharide in Bacillus anthracis.
Academic Article FmhA and FmhC of Staphylococcus aureus incorporate serine residues into peptidoglycan cross-bridges.
Academic Article Glycosylation-dependent opsonophagocytic activity of staphylococcal protein A antibodies.
Academic Article The Expression of von Willebrand Factor-Binding Protein Determines Joint-Invading Capacity of Staphylococcus aureus, a Core Mechanism of Septic Arthritis.

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