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One or more keywords matched the following properties of Horne-Badovinac, Sally
keywords Drosophila
overview My lab takes an integrative approach to studying organ morphogenesis. We are currently probing how cells migrate collectively & how basement membrane ECMs are assembled. Collective cell migration - The collective migration of cells within an epithelial sheet underlies tissue remodeling events associated with morphogenesis, wound repair, and the spread of many cancers. Yet little is known about how each epithelial cell coordinates its individual migratory behaviors with those of its neighbors, or how epithelial motility shapes tissues during development. We recently discovered a novel planar signaling system that coordinates leading and trailing edge dynamics between neighboring epithelial cells, and are now working to elucidate the mechanistic basis of this signaling. We are also investigating how collective cell migration can be harnesses to create a stable, planar tissue pattern within an epithelium for subsequent morphogenesis. Basement membrane dynamics – Basement membrane is a sheet-like extracellular matrix that lines the basal surface of all epithelial tissues. This complex protein network provides structural stability to the monolayer, promotes cell-cell and cell-matrix signaling, and acts as a physical barrier to metastasis. Despite their ubiquity and many essential functions, we know surprisingly little about how basement membranes are built or how they are remodeled during development. We are taking a comprehensive approach to identifying: the cellular mechanisms underlying basement membrane biosynthesis and secretion, how basement membrane biosynthetic programs are modulated to construct diverse matrix architectures, and how different basement membrane architectures ultimately influence organ morphogenesis. Experimental system – We perform these studies in the Drosophila egg chamber, which is an organ-like structure in the ovary that will give rise to one egg. Each egg chamber is comprised of a germ cell cluster, surrounded by a somatic epithelium of follicle cells. The follicle cells produce their own basement membrane, which ensheaths the organ. Though initially spherical, egg chambers lengthen as they mature. This morphogenesis depends on a dramatic collective migration of the follicle cells across their basement membrane, which causes the entire egg chamber to rotate within its surrounding matrix. Importantly, the follicle cells also remodel the basement membrane as they crawl along its surface, creating a polarized array of fibrils in the direction of tissue movement. This fibrillar matrix is then thought to act as a “molecular corset” that resists the expansive growth of the germ cells to directionally bias egg chamber growth, and thus create the elongated shape of the egg. A major strength of this system is that the collective migration and basement membrane remodeling both occur on the egg chamber’s outer surface, which allows us to image dynamic cell behaviors - in a living organ - with exquisite clarity. Moreover, we can use the sophisticated genetic tools of Drosophila to identify the underlying molecular mechanisms. Please check out the papers below to see what we have already discovered!
One or more keywords matched the following items that are connected to Horne-Badovinac, Sally
Item TypeName
Concept Drosophila melanogaster
Concept Drosophila Proteins
Academic Article Developmental biology. Tubular transformations.
Academic Article A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis.
Academic Article The Drosophila egg chamber-a new spin on how tissues elongate.
Academic Article Fat2 and Lar Define a Basally Localized Planar Signaling System Controlling Collective Cell Migration.
Academic Article Fat-like cadherins in cell migration-leading from both the front and the back.
Academic Article Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation.
Academic Article Influence of ovarian muscle contraction and oocyte growth on egg chamber elongation in Drosophila.
Academic Article Dynamic regulation of basement membrane protein levels promotes egg chamber elongation in Drosophila.
Academic Article Rab10-Mediated Secretion Synergizes with Tissue Movement to Build a Polarized Basement Membrane Architecture for Organ Morphogenesis.
Academic Article Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.
Academic Article Mass transit: epithelial morphogenesis in the Drosophila egg chamber.
Academic Article Building from the Ground up: Basement Membranes in Drosophila Development.
Academic Article Round and round gets you somewhere: collective cell migration and planar polarity in elongating Drosophila egg chambers.
Academic Article Dynein regulates epithelial polarity and the apical localization of stardust A mRNA.
Academic Article Shaping the Drosophila egg.
Academic Article A screen for round egg mutants in Drosophila identifies tricornered, furry, and misshapen as regulators of egg chamber elongation.
Academic Article Cultivation and Live Imaging of Drosophila Ovaries.
Academic Article Tissue Structure: A CIVICs Lesson for Adipocytes.
Academic Article Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila.
Academic Article The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development.
Academic Article Looking deeper into tissue elongation.
Academic Article Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation.
Academic Article The Drosophila micropyle as a system to study how epithelia build complex extracellular structures.
Academic Article ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Academic Article In-silico definition of the Drosophila melanogaster matrisome.
Academic Article DAAM mediates the assembly of long-lived, treadmilling stress fibers in collectively migrating epithelial cells in Drosophila.
Academic Article Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells.
Academic Article Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration.
Academic Article A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers During Drug Treatments.
Academic Article Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells.
Grant Genetic Analysis of Epithelial Morphogenesis and Organ Shape
Grant Mechanisms that Coordinate Cellular Movements for Epithelial Migration
Grant Mechanisms of basement membrane secretion and assembly
Academic Article Fat2 polarizes Lar and Sema5c to coordinate the motility of collectively migrating epithelial cells.
Academic Article Cortical tension promotes Kibra degradation via Par-1.
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  • Drosophila