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One or more keywords matched the following properties of Kovar, David R.
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keywords TIRF Microscopy
overview Cells simultaneously assemble and maintain different actin cytoskeleton networks of distinct organizations and dynamics for fundamental processes such as polarization, endocytosis, motility and division. How does a shared pool of actin monomer building blocks self-organize into distinct actin filament networks of specific densities, sizes and function within a common cellular cytoplasm? Our research group is investigating the molecular mechanisms that govern how diverse actin cytoskeleton networks self-organize through (1) competition for a limited supply of actin monomers, (2) network-specific sorting of actin binding proteins, and (3) the effects of different combinations of actin binding proteins on actin filament network organization, dynamics and function. We take advantage of complementary experiments in live cells and with purified components. These interdisciplinary techniques include genetics, time-lapse fluorescence microscopy of cells, biochemistry, biophysics, in vitro reconstitution and multi-color single molecule TIRF microscopy imaging.
One or more keywords matched the following items that are connected to Kovar, David R.
Item TypeName
Concept Microscopy, Video
Concept Microscopy, Confocal
Concept Microscopy, Fluorescence
Academic Article Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces.
Academic Article Control of the assembly of ATP- and ADP-actin by formins and profilin.
Academic Article Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo.
Academic Article Arabidopsis capping protein (AtCP) is a heterodimer that regulates assembly at the barbed ends of actin filaments.
Academic Article Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.
Academic Article Role of tropomyosin in formin-mediated contractile ring assembly in fission yeast.
Academic Article Minimal requirements for actin filament disassembly revealed by structural analysis of malaria parasite actin-depolymerizing factor 1.
Academic Article Assembly kinetics determine the architecture of a-actinin crosslinked F-actin networks.
Academic Article Identification and characterization of a small molecule inhibitor of formin-mediated actin assembly.
Academic Article The functionally distinct fission yeast formins have specific actin-assembly properties.
Academic Article Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism.
Academic Article Profilin-1 serves as a gatekeeper for actin assembly by Arp2/3-dependent and -independent pathways.
Academic Article Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.
Academic Article Attachment conditions control actin filament buckling and the production of forces.
Academic Article Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.
Academic Article Ena/VASP Enabled is a highly processive actin polymerase tailored to self-assemble parallel-bundled F-actin networks with Fascin.
Academic Article The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.
Academic Article In Vitro Biochemical Characterization of Cytokinesis Actin-Binding Proteins.
Academic Article Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly.
Academic Article Ena/VASP processive elongation is modulated by avidity on actin filaments bundled by the filopodia cross-linker fascin.
Academic Article Cooperation between tropomyosin and a-actinin inhibits fimbrin association with actin filament networks in fission yeast.
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  • Microscopy