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One or more keywords matched the following properties of He, Tong-Chuan

keywords Mesenchymal Stem Cells
keywords BMP signaling
keywords Wnt signaling
keywords Gene/cell-based therapy
overview I direct the Molecular Oncology Laboratory in the Department of Orthopaedic Surgery and Rehabilitation Medicine. Our lab consists of a dynamic group of basic science investigators and physician scientists, who have diverse expertise and a broad range of research interests. Our shared long-term objective is to better understand the molecular pathogenesis of bone and musculoskeletal diseases and to translate basic discoveries into potential therapies and/or preventive measures in clinical settings. Specifically, we focus on the following areas: LINEAGE COMMITMENT AND TERMINAL DIFFERENTIATION OF MESENCHYMAL STEM CELLS (MSCS) Multipotency of MSCs MSCs are pluripotent and capable of differentiating into osteogenic, chondrogenic, myogenic or adipogenic lineage. Understanding the molecular mechanisms of bone formation is pivotal for studying the pathogenesis of bone diseases. We are interested in elucidating the molecular mechanisms through which regulate the proliferation and differentiation of osteoblasts, chondrocytes, and adipocytes. Both Wnt/beta-catenin and bone morphogenetic proteins (BMPs) have been considered as important regulators of osteogenic differentiation of mesenchymal stem cells. We are investigating the biological functions of BMPs and Wnt/beta-catenin signaling in regulating the osteoblast differentiation of MSCs. We have therefore conducted a comprehensive analysis of BMP and Wnt3A-induced osteoblast differentiation of MSCs. Our findings indicate that osteoblast differentiation is a well-regulated process in normal progenitor cells, while osteosarcoma cells are refractory to osteogenic differentiation signal. BMP-9 is one of the most potent regulators of osteogenic differentiation The bone-forming osteoblasts are derived from pluripotent bone marrow fibroblasts (a.k.a., MSCs). Although the molecular mechanisms underlying bone formation remain to be defined, BMPs seem to play an important role in osteoblast differentiation. At least 15 types of BMPs have been identified in mice and humans. However, the ability of BMPs to induce osteoblast differentiation of mesenchymal stem cells has not been comprehensively investigated, mostly due to the fact that recombinant proteins are either not biologically active or not available for all BMPs. Through a comprehensive analysis, we found that BMP2, BMP6, and BMP9 (to a much lesser extent, BMP4 and BMP7) exhibited the greatest ability to induce osteoblast differentiation, whereas BMP3 was shown to inhibit osteogenesis, both in vitro and in vivo. Our findings about BMP9 as one of the most potent inducers of osteoblast differentiation are novel and particularly intriguing because BMP9 is one of the least characterized BMPs and its functions are largely unknown. Thus, many questions regarding BMP9 signaling need to be answered: What are the type I and type II TGFbeta/BMP receptors involved in BMP9 binding? What Smads are required for BMP9 signaling? How are the downstream targets regulated by BMP9? More importantly, what are the roles of BMP9 during embryonic and/or skeletal development? We are interested in addressing these questions. Critical mediators of BMP-induced differentiation of MSCs We have identified several potentially important signaling mediators of BMP-induced osteogenic differentiation. These targets include the Inhibitors of DNA binding/Differentiation helix-loop-helix (a.k.a., Id proteins) and CTGF. Interestingly, both Ids and CTGF have been shown to overexpress in human tumors. Our findings suggest that Ids and CTGF may play an important role in promoting the proliferation of early osteoblast progenitor cells and that their expression must be down-regulated during the terminal differentiation of committed osteoblasts, suggesting that a balanced regulation of their expression may be critical to BMP-induced osteoblast lineage-specific differentiation of MSCs. We are currently dissecting the functional roles of the important downstream mediators in BMP-induced osteogenic signaling. We are also interested in identifying the molecular switches that control the lineage-specific differentiation of osteoblasts, chondrocytes, and adipocytes in MSCs. We are especially interested in understanding at what stage osteogenic BMPs (e.g., BMP2 or BMP9)-induced osteogenic and adipogenic differentiation diverges. What are the potential regulators that control this lineage divergence? It has been long postulated that a disrupted balance between osteogenesis and adipogenesis may cause musculoskeletal diseases, such as osteoporosis. Wnts regulate osteogenic differentiation of mesenchymal stem cells Our earlier studies demonstrated that Wnt/beta-catenin signaling is de-regulated in over 70% of human osteosarcoma. Recent studies also suggest that Wnt signaling may play an important role in regulating bone density, and one of the Wnt signaling antagonists Dkk1 may be implicated in the development of osteolytic lesion in multiple myeloma patients. We demonstrated that Wnt3A can induce the early osteogenic marker alkaline phosphatase in MSCs. Upon analyzing the gene expression profile of MSCs that were stimulated with Wnt3A, we found that three members of the CCN family, CCN1/Cyr61, CCN2/CTGF, and CCN5/WISP2, were among the most significantly up-regulated genes. Further studies demonstrate that CTGF is a mutual target of Wnt and BMP-9, and plays an important role in regulating osteogenic differentiation. However, more questions remain to be answered. We are currently investigating how osteogenic differentiation of MSCs is regulated by canonical and non-canonical Wnt signaling. MOLECULAR BASES OF BONE AND SOFT TISSUE SARCOMAS Sarcomas represent a heterogeneous group of malignant mesenchymal tumors with numerous histologic subtypes and complex cytogenetic abnormalities. Unlike other common solid tumors, sarcomas are relatively uncommon and their molecular pathogenesis, in general, are poorly understood. We mainly focus on understanding the molecular biology of primary bone tumor (a.k.a., osteosarcoma, OS). Osteosarcoma is the most common primary malignancy of bone, and is among the most common non-hematological primary tumors of bone in both children and adults. The peak incidence occurs in the second decade. With preoperative chemotherapy, the five-year survival rate of patients with non-metastatic tumors has improved significantly to approximately 60-70%. However, cases with local recurrence and/or pulmonary metastasis are usually less sensitive, if not resistant, to conventional chemotherapies. Several cytogenetic studies suggest that certain chromosomal regions may be amplified, rearranged, or deleted in some, but not all, human osteosarcomas. Unfortunately, the low incidence of this disease and the absence of any familial predisposition have complicated efforts to identify osteosarcoma-associated genes. Thus, we are taking a comprehensive approach to elucidate the molecular mechanisms underlying the development of osteosarcoma. Wnt/beta-catenin signaling in the development of human osteosarcoma Aberrant activation of Wnt/beta-catenin signaling by inactivating APC tumor suppressor or oncogenic activation of beta-catenin plays an important role in colorectal tumorigenesis. Oncogenic activation of beta-catenin has also been reported in several types of human solid tumors. We found that beta-catenin signaling is de-regulated in about 70% of human osteosarcoma without beta-catenin mutations. As in many other types of non-colon cancer, little is known about how Wnt/beta-catenin signaling pathway is activated. Therefore, we are interested in elucidating the upstream regulatory mechanisms that may underline of the beta-catenin signaling pathway. We are also interested in investigating the possible pathogenic role of beta-catenin deregulation in bone and soft tissue tumors. Furthermore, we are investigating the potential roles of EF-hand calcium-binding S100 proteins in osteosarcoma progression and the development of pulmonary metastasis. Clinically relevant osteosarcoma animal model In order to investigate the pathogenesis of human osteosarcoma, there is a great need to develop a clinically relevant animal model. We have developed such an orthotopic animal model of osteosarcoma using several human osteosarcoma lines. This clinically relevant model of human osteosarcoma provides varying degrees of tumor growth at the primary site and of metastatic potential. Thus, this orthotopic model is being used as a valuable tool to investigate factors that promote or inhibit osteosarcoma growth and/or metastasis. For instance, we are now using this model to investigate the roles of genes that are known to promote cell proliferation and inhibit differentiation, and genes that potentially promote or inhibit cancer metastasis. This orthotopic model can also be used to test anti-tumor small molecules or other targeted therapies. Osteosarcoma is a differentiation disease We believe that understanding the molecular events behind normal osteoblast differentiation of MSCs may provide important clues to osteosarcoma development. Stem cell differentiation and tumorigenesis share some strikingly similar characteristics. Both normal stem cells and cancer stem cells have the ability to self-renew. Although stem cells are often the target of genetic events that are necessary or sufficient for malignant transformation, in some cases restricted progenitors or even differentiated cells may become transformed. Thus, cancer stem cells may be derived from tissue-specific stem cells or progenitors, such as MSCs. Although cancer stem cells for osteosarcoma remain to be identified, OS cells indeed exhibit the characteristics of undifferentiated osteoblasts, and we have shown that differentiation-promoting agents can inhibit OS proliferation. Accordingly, we found that human osteosarcoma cells are in general refractory to osteogenic BMPs and exhibit no signs of terminal differentiation upon osteogenic BMP stimulation. Thus, we hypothesize that osteosarcoma development is caused by molecular/genetic disruptions of the osteogenic differentiation pathway. We are investigating and identifying the possible defects in osteogenic pathway, including the possible roles of the early target genes of BMP-induced osteoblast lineage-specific differentiation of MSCs. MOLECULAR BIOLOGY OF CANCER METASTASIS Cancer metastasis is an often overlooked and least understood problem. Metastasis is defined as the progressive growth of tumor cells at a site that is discontinuous from the primary tumor. Although not an efficient process, colonization at distant tissues by tumor cells represents the most dangerous attribute of cancer, because metastases, rather than primary tumors, are responsible for most cancer deaths. Metastatic cells are a subset of primary tumor cells that have acquired the ability to complete a multi-step metastatic cascade, including migration, dissemination, extravasation, and eventual proliferation at a discontinuous secondary site. Understanding the molecular biology of cancer metastasis may provide novel intervention strategies to control/contain metastatic lesions, and/or to improve the quality of life for the patients with these advanced diseases. Pulmonary metastasis of primary bone tumors Lung metastasis is the leading cause of OS mortality. Our orthotopic tumor model of human OS provides a unique opportunity for us to investigate the molecular events underlying osteosarcoma pulmonary metastasis. Using this model, we have conducted serial selections of highly metastatic human OS sublines, which were otherwise non-metastatic. Using microarray analysis, we have compared the gene expression profiles between these sublines and the parental lines in search for gene(s) that may cause or be associated with osteosarcoma metastasis. In an alternative approach, we are conducting functional selection assays, in which an RNAi library has been introduced into the non-metastatic or less metastatic human osteosarcoma cells to recover lung metastases and to identify potential metastasis suppressors of human osteosarcoma. Metastatic bone tumors Bone is one of the most frequently targeted organs for cancer metastasis, and bone is the most common site for distant relapse of most cancers. The bone microenvironment is unique among metastatic target tissues because it is subjected to continuous remodeling under the influence circulating hormones and local bone-derived factors. Interactions between the bone microenvironment and the cancer cells can give rise to osteolytic (bone resorbing) or osteoblastic (bone forming) metastasis. Osteolytic bone metastasis are characteristic for most malignancies, such as breast cancer and lung cancer, while osteoblastic metastasis is mostly associated with prostate cancer. We are investigating the molecular mechanisms through which the interactions between metastatic (breast and prostate) cancer cells and bone microenvironment are regulated. NOVEL THERAPEUTIC AND/OR PREVENTIVE STRATEGIES FOR BONE AND MUSCULOSKELETAL DISEASES The ultimate goal of biomedical research is to develop innovative diagnostic, therapeutic, and/or preventive strategies for human diseases. We are interested in developing innovative approaches for local or systemic delivery of therapeutic genes as effective treatment for bone and musculoskeletal diseases. We are investigating the use of osteogenic BMPs for bone regeneration, enhancing fracture healing, repairing segmental defects, promoting spinal fusion, and preventing implant wear particle-induced osteolysis. We have recently demonstrated the potential use of some of the BMPs in promoting biomechanical features of healing tendons/ligaments. We also demonstrated that Sox9, a master regulator of chondrogenesis, may be used in gene therapy to treat intervertebral disc degeneration and articular cartilage injuries. These lines of investigation reflect our commitment to the true spirit of translational research in bone and musculoskeletal disorders.

One or more keywords matched the following items that are connected to He, Tong-Chuan

Item TypeName
Concept Bone Marrow Cells
Concept Carcinoma, Squamous Cell
Concept Cell Communication
Concept Cell Line, Transformed
Concept Cell Membrane Permeability
Concept Cell Differentiation
Concept Cell Movement
Concept Cell Nucleus
Concept Carcinoma, Renal Cell
Concept Cell Membrane
Concept Cell Division
Concept Cell Separation
Concept Eukaryotic Cells
Concept Clone Cells
Concept Cell Adhesion
Concept Cell Cycle
Concept Cell Transformation, Neoplastic
Concept Cell Aggregation
Concept Cell Fusion
Concept Epithelial Cells
Concept HeLa Cells
Concept Stem Cells
Concept Tumor Stem Cell Assay
Concept Cell Culture Techniques
Concept Tumor Cells, Cultured
Concept Cell Adhesion Molecules
Concept Stem Cell Transplantation
Concept Cell Transplantation
Concept Cell Proliferation
Concept Embryonic Stem Cells
Concept Intercellular Signaling Peptides and Proteins
Concept Primary Cell Culture
Concept NIH 3T3 Cells
Concept Cell Cycle Proteins
Concept Caco-2 Cells
Concept Active Transport, Cell Nucleus
Concept Mesenchymal Stem Cell Transplantation
Concept HCT116 Cells
Concept Multipotent Stem Cells
Concept Pluripotent Stem Cells
Concept 3T3-L1 Cells
Concept CCN Intercellular Signaling Proteins
Concept HEK293 Cells
Concept Adaptor Proteins, Signal Transducing
Concept Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Concept Cell Death
Concept Cell Hypoxia
Concept Proliferating Cell Nuclear Antigen
Concept HT29 Cells
Concept Cell Lineage
Concept MAP Kinase Signaling System
Concept Extracellular Signal-Regulated MAP Kinases
Concept Cell Cycle Checkpoints
Concept Hep G2 Cells
Concept Endothelial Cells
Concept Cell Line, Tumor
Concept Intracellular Signaling Peptides and Proteins
Concept Cell Shape
Concept Wnt Signaling Pathway
Concept Cell Line
Concept Cell Survival
Concept Cells, Cultured
Concept Neoplastic Stem Cells
Concept Signal Transduction
Concept G2 Phase Cell Cycle Checkpoints
Academic Article Nuclear receptor agonists as potential differentiation therapy agents for human osteosarcoma.
Academic Article Tyrosine kinase inhibitor STI-571/Gleevec down-regulates the beta-catenin signaling activity.
Academic Article Cytoplasmic and/or nuclear accumulation of the beta-catenin protein is a frequent event in human osteosarcoma.
Academic Article [The expression of Recombinant adenovirus IkappaBalphaM in human hepatocarcinoma HepG2 and it's inhibitive effect to the activity of NF-kappaB].
Academic Article Elevated protein expression of cyclin D1 and Fra-1 but decreased expression of c-Myc in human colorectal adenocarcinomas overexpressing beta-catenin.
Academic Article Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells.
Academic Article Transcriptional characterization of bone morphogenetic proteins (BMPs)-mediated osteogenic signaling.
Academic Article Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells.
Academic Article Potential use of Sox9 gene therapy for intervertebral degenerative disc disease.
Academic Article An orthotopic model of human osteosarcoma growth and spontaneous pulmonary metastasis.
Academic Article Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc.
Academic Article Is cytomegalovirus associated with human colorectal tumorigenesis?
Academic Article Steamed American ginseng berry: ginsenoside analyses and anticancer activities.
Academic Article [Inhibition of HBV replication and antigen expression by RNA interference against different targets].
Academic Article Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells.
Academic Article Wnt signaling and human diseases: what are the therapeutic implications?
Academic Article Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells.
Academic Article S100A6 expression and function in human osteosarcoma.
Academic Article Regulation of osteogenic differentiation during skeletal development.
Academic Article Red American ginseng: ginsenoside constituents and antiproliferative activities of heat-processed Panax quinquefolius roots.
Academic Article Tyrosine kinase inhibitor STI-571: the new wonder drug of cancer therapy.
Academic Article BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling.
Academic Article Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects.
Academic Article Antiproliferative effects of different plant parts of Panax notoginseng on SW480 human colorectal cancer cells.
Academic Article Wnt antagonist SFRP3 inhibits the differentiation of mouse hepatic progenitor cells.
Academic Article In vitro and in vivo anticancer effects of American ginseng berry: exploring representative compounds.
Academic Article American ginseng berry enhances chemopreventive effect of 5-FU on human colorectal cancer cells.
Academic Article Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells.
Academic Article Neurogenesis-related genes expression profiling of mouse fibroblastic stem cells induced by Wnt signaling.
Academic Article Retinoic acid signalling induces the differentiation of mouse fetal liver-derived hepatic progenitor cells.
Academic Article Primary bovine intervertebral disc cells transduced with adenovirus overexpressing 12 BMPs and Sox9 maintain appropriate phenotype.
Academic Article Synergistic antitumor effect of the activated PPARgamma and retinoid receptors on human osteosarcoma.
Academic Article Establishment and characterization of a new highly metastatic human osteosarcoma cell line.
Academic Article A traditional Chinese medicine formulation consisting of Rhizoma Corydalis and Rhizoma Curcumae exerts synergistic anti-tumor activity.
Academic Article Retinoic acids potentiate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells.
Academic Article Immunosuppressive cyclosporin A activates AKT in keratinocytes through PTEN suppression: implications in skin carcinogenesis.
Academic Article Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells.
Academic Article Insulin-like growth factor 2 (IGF-2) potentiates BMP-9-induced osteogenic differentiation and bone formation.
Academic Article Pre-activation of retinoid signaling facilitates neuronal differentiation of mesenchymal stem cells.
Academic Article Low serum concentration facilitates the differentiation of hepatic progenitor cells.
Academic Article The therapeutic potential of the Wnt signaling pathway in bone disorders.
Academic Article Selection and validation of optimal siRNA target sites for RNAi-mediated gene silencing.
Academic Article Tetrandrine inhibits Wnt/ß-catenin signaling and suppresses tumor growth of human colorectal cancer.
Academic Article Comparative effects of bone morphogenetic proteins and Sox9 overexpression on matrix accumulation by bovine anulus fibrosus cells: implications for anular repair.
Academic Article Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells.
Academic Article Ginsenoside Rg3 inhibits colorectal tumor growth through the down-regulation of Wnt/ß-catenin signaling.
Academic Article Antioxidants potentiate American ginseng-induced killing of colorectal cancer cells.
Academic Article BMP9 inhibits the proliferation and invasiveness of breast cancer cells MDA-MB-231.
Academic Article LIGHT delivery to tumors by mesenchymal stem cells mobilizes an effective antitumor immune response.
Academic Article Crosstalk between NF-kappaB and beta-catenin pathways in bacterial-colonized intestinal epithelial cells.
Academic Article The CCN proteins: important signaling mediators in stem cell differentiation and tumorigenesis.
Academic Article Potent and specific inhibition of SARS-CoV antigen expression by RNA interference.
Academic Article Chemopreventive effects of heat-processed Panax quinquefolius root on human breast cancer cells.
Academic Article TGFbeta/BMP type I receptors ALK1 and ALK2 are essential for BMP9-induced osteogenic signaling in mesenchymal stem cells.
Academic Article CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells.
Academic Article All-trans retinoic acid inhibits tumor growth of human osteosarcoma by activating Smad signaling-induced osteogenic differentiation.
Academic Article Epigenetic regulation of mesenchymal stem cells: a focus on osteogenic and adipogenic differentiation.
Academic Article Enhancement of canonical Wnt/ß-catenin signaling activity by HCV core protein promotes cell growth of hepatocellular carcinoma cells.
Academic Article Biphasic effects of TGFß1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells.
Academic Article Lysophosphatidic acid acyltransferase ß (LPAATß) promotes the tumor growth of human osteosarcoma.
Academic Article Mesenchymal Progenitor Cells and Their Orthopedic Applications: Forging a Path towards Clinical Trials.
Academic Article BMP-9 induced osteogenic differentiation of mesenchymal stem cells: molecular mechanism and therapeutic potential.
Academic Article Mesenchymal stem cells: Molecular characteristics and clinical applications.
Academic Article The first identification of lysine malonylation substrates and its regulatory enzyme.
Academic Article A protocol for rapid generation of recombinant adenoviruses using the AdEasy system.
Academic Article Osteosarcoma development and stem cell differentiation.
Academic Article A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells.
Academic Article Oncolytic adenoviral vectors which employ the survivin promoter induce glioma oncolysis via a process of beclin-dependent autophagy.
Academic Article Expression of the Arf tumor suppressor gene is controlled by Tgfbeta2 during development.
Academic Article Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells.
Academic Article Differentiation of osteoprogenitor cells is induced by high-frequency pulsed electromagnetic fields.
Academic Article Conditional immortalization establishes a repertoire of mouse melanocyte progenitors with distinct melanogenic differentiation potential.
Academic Article Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs).
Academic Article Dual regulation of gene expression mediated by tetracycline and Cre recombinase.
Academic Article Wnt/beta-catenin signaling pathway as a novel cancer drug target.
Academic Article Increased expression of S100A6 is associated with decreased metastasis and inhibition of cell migration and anchorage independent growth in human osteosarcoma.
Academic Article Comparative effects of bone morphogenetic proteins and sox9 overexpression on extracellular matrix metabolism of bovine nucleus pulposus cells.
Academic Article Osteosarcoma and osteoblastic differentiation: a new perspective on oncogenesis.
Academic Article RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells.
Academic Article Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model.
Academic Article Adenoviral vectors.
Academic Article Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53.
Academic Article Conditionally immortalized mouse embryonic fibroblasts retain proliferative activity without compromising multipotent differentiation potential.
Academic Article Growth hormone synergizes with BMP9 in osteogenic differentiation by activating the JAK/STAT/IGF1 pathway in murine multilineage cells.
Academic Article Caspase-mediated pro-apoptotic interaction of panaxadiol and irinotecan in human colorectal cancer cells.
Academic Article Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway.
Academic Article Bone morphogenetic proteins in craniofacial surgery: current techniques, clinical experiences, and the future of personalized stem cell therapy.
Academic Article Wnt signaling in bone formation and its therapeutic potential for bone diseases.
Academic Article BMP9 signaling in stem cell differentiation and osteogenesis.
Academic Article The synergistic apoptotic interaction of panaxadiol and epigallocatechin gallate in human colorectal cancer cells.
Academic Article Ginseng saponin metabolite 20(S)-protopanaxadiol inhibits tumor growth by targeting multiple cancer signaling pathways.
Academic Article Paraptosis and NF-?B activation are associated with protopanaxadiol-induced cancer chemoprevention.
Academic Article Distinct osteogenic activity of BMPs and their orthopaedic applications.
Academic Article Genistein induces G2/M cell cycle arrest and apoptosis via ATM/p53-dependent pathway in human colon cancer cells.
Academic Article BMP9-regulated angiogenic signaling plays an important role in the osteogenic differentiation of mesenchymal progenitor cells.
Academic Article IGFBP5 domains exert distinct inhibitory effects on the tumorigenicity and metastasis of human osteosarcoma.
Academic Article Establishment and characterization of the reversibly immortalized mouse fetal heart progenitors.
Academic Article [Effect of BMP-2 and -3 overexpression on osteogenic and chondrogenic differentiation of prenatal mouse intervertebral disc cells in vitro].
Academic Article TERT promoter mutations occur frequently in gliomas and a subset of tumors derived from cells with low rates of self-renewal.
Academic Article Regulation of cell proliferation and migration by p62 through stabilization of Twist1.
Academic Article The piggyBac transposon-mediated expression of SV40 T antigen efficiently immortalizes mouse embryonic fibroblasts (MEFs).
Academic Article Reversibly Immortalized Mouse Articular Chondrocytes Acquire Long-Term Proliferative Capability While Retaining Chondrogenic Phenotype.
Academic Article Crosstalk between Wnt/ß-catenin and estrogen receptor signaling synergistically promotes osteogenic differentiation of mesenchymal progenitor cells.
Academic Article Endoplasmic reticulum (ER) stress inducible factor cysteine-rich with EGF-like domains 2 (Creld2) is an important mediator of BMP9-regulated osteogenic differentiation of mesenchymal stem cells.
Academic Article Characterization of chondrocyte scaffold carriers for cell-based gene therapy in articular cartilage repair.
Academic Article Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells.
Academic Article Characterization of scaffold carriers for BMP9-transduced osteoblastic progenitor cells in bone regeneration.
Academic Article Characterization of constitutive promoters for piggyBac transposon-mediated stable transgene expression in mesenchymal stem cells (MSCs).
Academic Article Noggin resistance contributes to the potent osteogenic capability of BMP9 in mesenchymal stem cells.
Academic Article Multipotential differentiation of human urine-derived stem cells: potential for therapeutic applications in urology.
Academic Article Functional characteristics of reversibly immortalized hepatic progenitor cells derived from mouse embryonic liver.
Academic Article Comparison of proliferation and differentiation potential between mouse primary hepatocytes and embryonic hepatic progenitor cells in vitro.
Academic Article BMP9 regulates cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells.
Academic Article A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA Assembly.
Academic Article Decellularized liver scaffolds effectively support the proliferation and differentiation of mouse fetal hepatic progenitors.
Academic Article S100A8 and S100A9 are associated with colorectal carcinoma progression and contribute to colorectal carcinoma cell survival and migration via Wnt/ß-catenin pathway.
Academic Article Brain-derived neurotrophic factor increases expression of MnSOD in human circulating angiogenic cells.
Academic Article Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs).
Academic Article Dihydroartemisinin inhibits tumor growth of human osteosarcoma cells by suppressing Wnt/ß-catenin signaling.
Academic Article Protopanaxadiol, an active ginseng metabolite, significantly enhances the effects of fluorouracil on colon cancer.
Academic Article Inhibition of histone deacetylases potentiates BMP9-induced osteogenic signaling in mouse mesenchymal stem cells.
Academic Article TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol.
Academic Article Hedgehog signaling is involved in the BMP9-induced osteogenic differentiation of mesenchymal stem cells.
Academic Article BMP9 inhibits the bone metastasis of breast cancer cells by downregulating CCN2 (connective tissue growth factor, CTGF) expression.
Academic Article The E-F hand calcium-binding protein S100A4 regulates the proliferation, survival and differentiation potential of human osteosarcoma cells.
Academic Article Destabilization of heterologous proteins mediated by the GSK3ß phosphorylation domain of the ß-catenin protein.
Academic Article Modulation of ß-catenin signaling by the inhibitors of MAP kinase, tyrosine kinase, and PI3-kinase pathways.
Academic Article BMP9 inhibits proliferation and metastasis of HER2-positive SK-BR-3 breast cancer cells through ERK1/2 and PI3K/AKT pathways.
Academic Article Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells.
Academic Article Bone morphogenetic protein-9 effectively induces osteo/odontoblastic differentiation of the reversibly immortalized stem cells of dental apical papilla.
Academic Article Targeting BMP9-promoted human osteosarcoma growth by inactivation of notch signaling.
Academic Article Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene.
Academic Article Adenovirus-mediated efficient gene transfer into cultured three-dimensional organoids.
Academic Article Bone morphogenetic protein 2 inhibits the proliferation and growth of human colorectal cancer cells.
Academic Article S100A9 promotes the proliferation and invasion of HepG2 hepatocellular carcinoma cells via the activation of the MAPK signaling pathway.
Academic Article 5-azacytidine promotes terminal differentiation of hepatic progenitor cells.
Academic Article Allogeneic Articular Chondrocyte Transplantation Downregulates Interleukin 8 Gene Expression in the Degenerating Rabbit Intervertebral Disk In Vivo.
Academic Article Characterization of Reversibly Immortalized Calvarial Mesenchymal Progenitor Cells.
Academic Article TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR.
Academic Article Three-dimensional co-culture of hepatic progenitor cells and mesenchymal stem cells in vitro and in vivo.
Academic Article The Anthelmintic Drug Niclosamide Inhibits the Proliferative Activity of Human Osteosarcoma Cells by Targeting Multiple Signal Pathways.
Academic Article Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells.
Academic Article Activation of PKA/CREB Signaling is Involved in BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells.
Academic Article High intensity focused ultrasound enhances anti-tumor immunity by inhibiting the negative regulatory effect of miR-134 on CD86 in a murine melanoma model.
Academic Article Antibiotic monensin synergizes with EGFR inhibitors and oxaliplatin to suppress the proliferation of human ovarian cancer cells.
Academic Article All-trans retinoic acid inhibits proliferation, migration, invasion and induces differentiation of hepa1-6 cells through reversing EMT in vitro.
Academic Article The Calcium-Binding Protein S100A6 Accelerates Human Osteosarcoma Growth by Promoting Cell Proliferation and Inhibiting Osteogenic Differentiation.
Academic Article A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues.
Academic Article The Prodomain-Containing BMP9 Produced from a Stable Line Effectively Regulates the Differentiation of Mesenchymal Stem Cells.
Academic Article The evolving roles of canonical WNT signaling in stem cells and tumorigenesis: implications in targeted cancer therapies.
Academic Article Immortalized Mouse Achilles Tenocytes Demonstrate Long-Term Proliferative Capacity While Retaining Tenogenic Properties.
Academic Article [Effects of Plasmid Fibroblast Growth Factor-2 Magnetic Chitosan Gelatin Microspheres on Proliferation and Differentiation of Mesenchymal Stem Cells].
Academic Article A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells.
Academic Article Nanoparticulate Mineralized Collagen Scaffolds and BMP-9 Induce a Long-Term Bone Cartilage Construct in Human Mesenchymal Stem Cells.
Academic Article Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development.
Academic Article A Blockade of IGF Signaling Sensitizes Human Ovarian Cancer Cells to the Anthelmintic Niclosamide-Induced Anti-Proliferative and Anticancer Activities.
Academic Article Bone morphogenetic protein 9 (BMP9) induces effective bone formation from reversibly immortalized multipotent adipose-derived (iMAD) mesenchymal stem cells.
Academic Article Hypoxia pathway and hypoxia-mediated extensive extramedullary hematopoiesis are involved in ursolic acid's anti-metastatic effect in 4T1 tumor bearing mice.
Academic Article Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system.
Academic Article For your eyes only: Harnessing human embryonic stem cell-derived retinal pigment epithelial cells to improve impaired vision.
Academic Article BMP signaling in mesenchymal stem cell differentiation and bone formation.
Academic Article Wnt/ß-catenin signaling plays an ever-expanding role in stem cell self-renewal, tumorigenesis and cancer chemoresistance.
Academic Article An effective treatment of experimental osteomyelitis using the antimicrobial titanium/silver-containing nHP66 (nano-hydroxyapatite/polyamide-66) nanoscaffold biomaterials.
Academic Article High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression.
Academic Article Anthelmintic mebendazole enhances cisplatin's effect on suppressing cell proliferation and promotes differentiation of head and neck squamous cell carcinoma (HNSCC).
Academic Article Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes.
Academic Article Repair of critical sized cranial defects with BMP9-transduced calvarial cells delivered in a thermoresponsive scaffold.
Academic Article NEL-Like Molecule-1 (Nell1) Is Regulated by Bone Morphogenetic Protein 9 (BMP9) and Potentiates BMP9-Induced Osteogenic Differentiation at the Expense of Adipogenesis in Mesenchymal Stem Cells.
Academic Article Characterization of retroviral infectivity and superinfection resistance during retrovirus-mediated transduction of mammalian cells.
Academic Article Notch Signaling Augments BMP9-Induced Bone Formation by Promoting the Osteogenesis-Angiogenesis Coupling Process in Mesenchymal Stem Cells (MSCs).
Academic Article Gelatin-Derived Graphene-Silicate Hybrid Materials Are Biocompatible and Synergistically Promote BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells.
Academic Article Inhibitory effects of BMP9 on breast cancer cells by regulating their interaction with pre-adipocytes/adipocytes.
Academic Article Wnt and BMP Signaling Crosstalk in Regulating Dental Stem Cells: Implications in Dental Tissue Engineering.
Academic Article Engineering the Rapid Adenovirus Production and Amplification (RAPA) Cell Line to Expedite the Generation of Recombinant Adenoviruses.
Academic Article CRISPR/Cas9-Mediated Genome Editing Corrects Dystrophin Mutation in Skeletal Muscle Stem Cells in a Mouse Model of Muscle Dystrophy.
Academic Article Epimedin C Promotes Vascularization during BMP2-Induced Osteogenesis and Tumor-Associated Angiogenesis.
Academic Article In vitro effect of microRNA-107 targeting Dkk-1 by regulation of Wnt/ß-catenin signaling pathway in osteosarcoma.
Academic Article lncRNA H19 mediates BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) through Notch signaling.
Academic Article Signal transduction in the erythropoietin receptor system.
Academic Article The extended box 2 subdomain of erythropoietin receptor is nonessential for Jak2 activation yet critical for efficient mitogenesis in FDC-ER cells.
Academic Article Inhibition of erythropoietin-induced mitogenesis by a kinase-deficient form of Jak2.
Academic Article CDX2 is mutated in a colorectal cancer with normal APC/beta-catenin signaling.
Academic Article [Regulatory effect of faciogenital dysplasia 6 gene on hepatic stem cell differentiation].
Academic Article TRAIL-R1 as a novel surface marker for circulating giant cell tumor of bone.
Academic Article The beta-catenin binding domain of adenomatous polyposis coli is sufficient for tumor suppression.
Academic Article 14-3-3sigma is a p53-regulated inhibitor of G2/M progression.
Academic Article Genetic determinants of p53-induced apoptosis and growth arrest.
Academic Article Converting cancer genes into killer genes.
Academic Article Erythropoietin-dependent inhibition of apoptosis is supported by carboxyl-truncated receptor forms and blocked by dominant-negative forms of Jak2.
Academic Article Dominant negative effects of a carboxy-truncated Jak2 mutant on Epo-induced proliferation and Jak2 activation.
Academic Article Neural EGF-like protein 1 (NELL-1): Signaling crosstalk in mesenchymal stem cells and applications in regenerative medicine.
Academic Article Induced maturation of hepatic progenitor cells in vitro.
Academic Article Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine.
Academic Article lncRNA H19 mediates BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) through Notch signaling.
Academic Article BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients.
Academic Article Erythropoietin-induced recruitment of Shc via a receptor phosphotyrosine-independent, Jak2-associated pathway.
Academic Article Association of the p85 regulatory subunit of phosphatidylinositol 3-kinase with an essential erythropoietin receptor subdomain.
Academic Article A simplified system for generating recombinant adenoviruses.
Academic Article The box1 domain of the erythropoietin receptor specifies Janus kinase 2 activation and functions mitogenically within an interleukin 2 beta-receptor chimera.
Academic Article Identification of c-MYC as a target of the APC pathway.
Academic Article PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs.
Academic Article BMP9-induced osteoblastic differentiation requires functional Notch signaling in mesenchymal stem cells.
Academic Article The cerebral cavernous malformation disease causing gene KRIT1 participates in intestinal epithelial barrier maintenance and regulation.
Academic Article Breast cancer development and progression: Risk factors, cancer stem cells, signaling pathways, genomics, and molecular pathogenesis.
Academic Article [Twist regulates proliferation, migration and invasion of osteosarcoma cells in vitro].
Academic Article A thermoresponsive, citrate-based macromolecule for bone regenerative engineering.
Academic Article A blockade of PI3K? signaling effectively mitigates angiotensin II-induced renal injury and fibrosis in a mouse model.
Academic Article Hepa1-6-FLuc cell line with the stable expression of firefly luciferase retains its primary properties with promising bioluminescence imaging ability.
Academic Article Sox9 augments BMP2-induced chondrogenic differentiation by downregulating Smad7 in mesenchymal stem cells (MSCs).
Academic Article Reversibly immortalized hepatic progenitor cell line containing double suicide genes.
Academic Article LncRNA ITGB2-AS1 Could Promote the Migration and Invasion of Breast Cancer Cells through Up-Regulating ITGB2.
Academic Article Niclosamide Exhibits Potent Anticancer Activity and Synergizes with Sorafenib in Human Renal Cell Cancer Cells.
Academic Article Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference.
Academic Article Periodic acid-Schiff staining method for function detection of liver cells is affected by 2% horse serum in induction medium.
Academic Article Inhibitor of ß-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/ß-catenin complex.
Academic Article Epigenetic silencing of SFRP5 promotes the metastasis and invasion of chondrosarcoma by expression inhibition and Wnt signaling pathway activation.
Academic Article CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs).
Academic Article Whole-Proteome Analysis of Human Craniosynostotic Tissue Suggests a Link between Inflammatory Signaling and Osteoclast Activation in Human Cranial Suture Patency.
Academic Article Reversibly immortalized human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are responsive to BMP9-induced osteogenic and adipogenic differentiation.
Academic Article A novel strategy for in vivo angiogenesis and osteogenesis: magnetic micro-movement in a bone scaffold.
Academic Article Effects of Brain-Derived Neurotrophic Factor on MicroRNA Expression Profile in Human Endothelial Progenitor Cells.
Academic Article Effects of senescence and angiotensin II on expression and processing of amyloid precursor protein in human cerebral microvascular endothelial cells.
Academic Article BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1.
Academic Article Monensin inhibits cell proliferation and tumor growth of chemo-resistant pancreatic cancer cells by targeting the EGFR signaling pathway.
Academic Article A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.
Academic Article Thermoresponsive Citrate-Based Graphene Oxide Scaffold Enhances Bone Regeneration from BMP9-Stimulated Adipose-Derived Mesenchymal Stem Cells.
Academic Article Bone Morphogenetic Protein-9-Stimulated Adipocyte-Derived Mesenchymal Progenitors Entrapped in a Thermoresponsive Nanocomposite Scaffold Facilitate Cranial Defect Repair.
Academic Article Dentinogenesis and Tooth-Alveolar Bone Complex Defects in BMP9/GDF2 Knockout Mice.
Academic Article A simplified system for the effective expression and delivery of functional mature microRNAs in mammalian cells.
Academic Article Osteoprotegerin reduces osteoclast resorption activity without affecting osteogenesis on nanoparticulate mineralized collagen scaffolds.
Academic Article Imiquimod Acts Synergistically with BMP9 through the Notch Pathway as an Osteoinductive Agent In Vitro.
Academic Article Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells.
Academic Article A simplified 3D liver microsphere tissue culture model for hepatic cell signaling and drug-induced hepatotoxicity studies.
Academic Article Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes.
Academic Article lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs.
Academic Article ARRB1-Promoted NOTCH1 Degradation Is Suppressed by OncomiR miR-223 in T-cell Acute Lymphoblastic Leukemia.
Academic Article Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Academic Article Stem cell therapy for chronic skin wounds in the era of personalized medicine: From bench to bedside.
Academic Article "Differential responsiveness to BMP9 between patent and fused suture progenitor cells from craniosynostosis patients."
Academic Article Correction for: Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Academic Article Transcriptomic landscape regulated by the 14 types of bone morphogenetic proteins (BMPs) in lineage commitment and differentiation of mesenchymal stem cells (MSCs).
Academic Article Leptin Potentiates BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells Through the Activation of JAK/STAT Signaling.
Academic Article The wonders of BMP9: From mesenchymal stem cell differentiation, angiogenesis, neurogenesis, tumorigenesis, and metabolism to regenerative medicine.
Academic Article Differential Responsiveness to BMP9 between Patent and Fused Suture Progenitor Cells from Craniosynostosis Patients.
Academic Article Highly expressed BMP9/GDF2 in postnatal mouse liver and lungs may account for its pleiotropic effects on stem cell differentiation, angiogenesis, tumor growth and metabolism.
Academic Article Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells.
Academic Article Chinese Herbal Medicine and Its Regulatory Effects on Tumor Related T Cells.
Academic Article Recombinant adenovirus (AdEasy system) mediated exogenous expression of long non-coding RNA H19 (lncRNA H19) biphasic regulating osteogenic differentiation of mesenchymal stem cells (MSCs).
Academic Article Role of Special AT-Rich Sequence-Binding Protein 2 in the Osteogenesis of Human Dental Mesenchymal Stem Cells.
Academic Article Cytotoxicity and Preliminary Analysis of the Pro-apoptotic and Cell Cycle Arrest Effects of Lantana ukambensis Against Colorectal Cancer Cells.
Academic Article Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue.
Academic Article FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells.
Academic Article All-trans-retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating autophagy.
Academic Article Blockade of IGF/IGF-1R signaling axis with soluble IGF-1R mutants suppresses the cell proliferation and tumor growth of human osteosarcoma.
Academic Article Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine.
Academic Article SP1-induced long non-coding RNA SNHG6 facilitates the carcinogenesis of chondrosarcoma through inhibiting KLF6 by recruiting EZH2.
Academic Article The inhibition of BRAF activity sensitizes chemoresistant human ovarian cancer cells to paclitaxel-induced cytotoxicity and tumor growth inhibition.
Academic Article Long non-coding RNA (LncRNA) HOTAIR regulates BMP9-induced osteogenic differentiation by targeting the proliferation of mesenchymal stem cells (MSCs).
Academic Article Notch signaling: Its essential roles in bone and craniofacial development.
Academic Article BMP9-initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/ß-catenin signalling activity.
Academic Article SNHG3 promotes migration, invasion, and epithelial-mesenchymal transition of breast cancer cells through the miR-186-5p/ZEB1 axis.
Academic Article BMP4 augments the survival of hepatocellular carcinoma (HCC) cells under hypoxia and hypoglycemia conditions by promoting the glycolysis pathway.
Academic Article Erratum: Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue.
Academic Article Hypoxia Preconditioning Promotes the Proliferation and Migration of Human Urine-Derived Stem Cells in Chronically Injured Liver of Mice by Upregulating CXCR4.
Academic Article Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering.
Academic Article Indocyanine Green Uptake and Periodic Acid-Schiff Staining Method for Function Detection of Liver Cells are Affected by Different Cell Confluence.
Academic Article Editorial: Extracellular Vesicle Treatment, Epigenetic Modification and Cell Reprogramming to Promote Bone and Cartilage Regeneration.
Academic Article Erratum to: Biphasic effects of TGFß1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells.

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