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One or more keywords matched the following properties of Carrillo, Robert
keywords Drosophila melanogaster
overview The long term goals of my lab are to understand the molecules and developmental programs that regulate neuronal development and wiring. To this end, we investigated the novel interactions between two subfamilies of the immunoglobulin superfamily in Drosophila melanogaster (in collaboration with Christopher Garcia at Stanford and Engin Ozkan at the University of Chicago; Ozkan et al., 2013): the 21-member Dprs and the 9-member DIPs. Previously, we found that an interacting Dpr-DIP pair functions at various developmental stages including motor neuron development at the larval neuromuscular junction (NMJ) and wiring and cell survival in the pupal optic lobe (Carrillo et al., 2015). In my lab, we will explore the functions of cell surface proteins, including Dprs and DIPs, and their downstream signaling cascades in nervous system development. Understanding these mechanisms will also contribute to our understanding of neurological diseases marked by alternations in connectivity such as autism spectrum disorder. Neuromuscular system: The larval neuromuscular circuit is highly stereotyped with single cell resolution due to the limited number of motor neurons (35) and muscle targets (30) in each hemisegment. Motor neurons in the ventral nerve cord must send their axons into the periphery and innervate their appropriate muscle target(s) in a highly stereotypic pattern. This system provides an ideal platform in which to tease apart the molecular determinants that contribute to this hard-wired specificity. We recently found that a Dpr-DIP pair controls the targeting of a specific motor neuron to its corresponding muscle. This unique phenotype will serve as a model to delve deeper into the molecules and mechanisms that function in Dpr-DIP regulated wiring using a combination of forward and reverse genetics, biochemistry, electrophysiology, behavioral assays, and cell culture studies. Ventral nerve cord: Upstream of muscle innervation, motor neurons receive input from interneurons in the ventral nerve cord (VNC; analogous to the vertebrate spinal cord). These interneurons integrate information from the central brain as well as sensory input in order to produce an appropriate motor response. Here we ask: does interneuron-motor neuron connectivity use similar mechanisms to those used in the neuromuscular system? Unlike the NMJ, these neuronal processes are not sparse enough to allow for single-cell resolution. However, we will utilize genetic tools that allow for single cell resolution of dendritic arbors and axon terminals when combined with confocal microscopy. Simultaneous optogenetic manipulation and calcium imagining, in addition to electrophysiology, will allow us to monitor perturbations in circuit function. Visual system: The fly visual circuit is composed of the retina, lamina, medulla, lobula, and lobula plate. Photoreceptors in the retina receive light stimuli and relay signals to downstream neurons which integrate that information to elicit an appropriate behavioral response. The laminar organization of synaptic connections, complete EM reconstruction of the fly brain, and a myriad of genetic tools provide an excellent system to interrogate the mechanisms underlying neural wiring. Dprs and DIPs are expressed in subsets of neurons in the visual circuit and synaptic partners express corresponding Dpr-DIP interacting pairs. Utilizing genetic and functional tools, we are investigating if Dpr-DIP combinations provide a cell-surface signature to specify synaptic partner matching.
One or more keywords matched the following items that are connected to Carrillo, Robert
Item TypeName
Concept Drosophila
Concept Drosophila Proteins
Academic Article The translational regulator Cup controls NMJ presynaptic terminal morphology.
Academic Article Is instability good for the brain?
Academic Article Development and plasticity of the Drosophila larval neuromuscular junction.
Academic Article Presynaptic activity and CaMKII modulate retrograde semaphorin signaling and synaptic refinement.
Academic Article Control of Synaptic Connectivity by a Network of Drosophila IgSF Cell Surface Proteins.
Academic Article An extracellular interactome of immunoglobulin and LRR proteins reveals receptor-ligand networks.
Academic Article Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit.
Academic Article Transsynaptic interactions between IgSF proteins DIP-a and Dpr10 are required for motor neuron targeting specificity in Drosophila
Academic Article Molecular basis of synaptic specificity by immunoglobulin superfamily receptors in Drosophila.
Academic Article Transsynaptic interactions between IgSF proteins DIP-a and Dpr10 are required for motor neuron targeting specificity.
Academic Article Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.
Academic Article Temporal transcription factors determine circuit membership by permanently altering motor neuron-to-muscle synaptic partnerships.
Academic Article Structural and Functional Synaptic Plasticity Induced by Convergent Synapse Loss in the Drosophila Neuromuscular Circuit.
Academic Article Systematic expression profiling of Dpr and DIP genes reveals cell surface codes in Drosophila larval motor and sensory neurons.
Academic Article Dpr10 and Nocte are required for Drosophila motor axon pathfinding.
Grant Interactions Between IgSF Proteins in Neural Circuit Formation
Grant IgSF protein interactions drive specificity in circuit wiring and synaptic elaboration
Academic Article Glial Draper signaling triggers cross-neuron plasticity in bystander neurons after neuronal cell death in Drosophila.
Academic Article hkb is required for DIP-a expression and target recognition in the Drosophila neuromuscular circuit.
Academic Article Neuronal Wiring Receptors Dprs and DIPs Are GPI Anchored and This Modification Contributes to Their Cell Surface Organization.
Academic Article Using the Proximity Ligation Assay to Visualize Colocalization of Proteins at the Drosophila Larval Neuromuscular Junction.
Academic Article Drosophila Late Embryonic through Late Larval Stage Body Wall Dissection: Dissection Tools and Techniques.
Academic Article hkb is required for DIP-a expression and target recognition in the Drosophila neuromuscular circuit.
Academic Article Immunohistochemistry and Morphometric Analysis of Drosophila Larval Body Wall Neuromuscular Junction Preparations.
Academic Article The Drosophila Larval Neuromuscular Junction: Developmental Overview.
Academic Article Cell Ablation Techniques for the Larval Drosophila Neuromuscular System.
Academic Article Labeling of Cell Surface Proteins at the Drosophila Larval Neuromuscular Junction Using Binding Partner Peptides.
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  • Drosophila