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Glick, Benjamin

One or more keywords matched the following properties of Glick, Benjamin

Property	Value
overview	Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.

Property

Value

overview

Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.

One or more keywords matched the following items that are connected to Glick, Benjamin

Item Type	Name
Concept	Alleles
Concept	Amino Acid Sequence
Concept	Base Sequence
Concept	Binding Sites
Concept	Cell-Free System
Concept	Saccharomycetales
Concept	Database Management Systems
Concept	Lac Operon
Concept	Disease Susceptibility
Concept	Models, Structural
Concept	Genes, Lethal
Concept	Genes, Recessive
Concept	Oncogenes
Concept	Molecular Sequence Data
Concept	Heat-Shock Proteins
Concept	Species Specificity
Concept	Solubility
Concept	Schizosaccharomyces
Concept	Stress, Mechanical
Concept	Sulfur Radioisotopes
Concept	Software
Concept	Spheroplasts
Concept	Sequence Tagged Sites
Concept	Sequence Homology, Amino Acid
Concept	Gene Library
Concept	Protein Structure, Quaternary
Concept	Protein Subunits
Concept	Genomic Library
Concept	Mutagenesis, Site-Directed
Concept	Sequence Alignment
Concept	Alternative Splicing
Concept	Sequence Analysis, DNA
Concept	Protein Structure, Tertiary
Concept	Genes, Reporter
Concept	Sequence Deletion
Concept	HSP70 Heat-Shock Proteins
Concept	Qa-SNARE Proteins
Concept	Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Concept	Calcium Signaling
Concept	Cellular Structures
Concept	N-Ethylmaleimide-Sensitive Proteins
Concept	SNARE Proteins
Concept	Schizosaccharomyces pombe Proteins
Concept	Saccharomyces cerevisiae Proteins
Concept	Rats, Inbred Strains
Concept	Saccharomyces cerevisiae
Concept	Spectrometry, Fluorescence
Concept	Urea
Concept	Insulin-Secreting Cells
Concept	Thiosulfate Sulfurtransferase
Concept	Open Reading Frames
Concept	Genome, Fungal
Concept	Ultracentrifugation
Concept	Blotting, Southern
Concept	Sequence Homology
Concept	Secretory Vesicles
Concept	Two-Hybrid System Techniques
Concept	Protein Sorting Signals
Concept	Transcriptome
Concept	Genome-Wide Association Study
Concept	Secretory Pathway
Concept	Molecular Sequence Annotation
Concept	Synthetic Biology
Concept	Cell Survival
Concept	Genes, Fungal
Concept	Serine Endopeptidases
Concept	Staining and Labeling
Concept	User-Computer Interface
Concept	Vesicular stomatitis Indiana virus
Concept	Signal Transduction
Concept	Information Storage and Retrieval
Concept	Macromolecular Substances
Academic Article	Hsp60-independent protein folding in the matrix of yeast mitochondria.
Academic Article	Isolation of highly purified mitochondria from Saccharomyces cerevisiae.
Academic Article	Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site.
Academic Article	The mitochondrial protein import motor: dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis.
Academic Article	Pathways and energetics of mitochondrial protein import in Saccharomyces cerevisiae.
Academic Article	Cell biology: alternatives to baker's yeast.
Academic Article	A cisternal maturation mechanism can explain the asymmetry of the Golgi stack.
Academic Article	A yeast t-SNARE involved in endocytosis.
Academic Article	Active unfolding of precursor proteins during mitochondrial protein import.
Academic Article	The curious status of the Golgi apparatus.
Academic Article	Saccharomyces cerevisiae mitochondria lack a bacterial-type sec machinery.
Academic Article	What is the driving force for protein import into mitochondria?
Academic Article	Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.
Academic Article	Protein sorting in mitochondria.
Academic Article	Barreling through the outer membrane.
Academic Article	Isolation of Pichia pastoris genes involved in ER-to-Golgi transport.
Academic Article	A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris.
Academic Article	Strong precursor-pore interactions constrain models for mitochondrial protein import.
Academic Article	Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of peroxisomes during micropexophagy.
Academic Article	A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
Academic Article	Raising the speed limits for 4D fluorescence microscopy.
Academic Article	Deconstructing Golgi inheritance.
Academic Article	Can the Golgi form de novo?
Academic Article	Monitoring changes in the subcellular location of proteins in S. cerevisiae.
Academic Article	The transitional ER localization mechanism of Pichia pastoris Sec12.
Academic Article	Vector for pop-in/pop-out gene replacement in Pichia pastoris.
Academic Article	Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed).
Academic Article	De novo formation of transitional ER sites and Golgi structures in Pichia pastoris.
Academic Article	The mechanisms of vesicle budding and fusion.
Academic Article	Sec16 is a determinant of transitional ER organization.
Academic Article	MICA: desktop software for comprehensive searching of DNA databases.
Academic Article	Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.
Academic Article	Golgi maturation visualized in living yeast.
Academic Article	Let there be order.
Academic Article	Protein import into isolated yeast mitochondria.
Academic Article	Cdc1p is an endoplasmic reticulum-localized putative lipid phosphatase that affects Golgi inheritance and actin polarization by activating Ca2+ signaling.
Academic Article	GRASPing unconventional secretion.
Academic Article	A noncytotoxic DsRed variant for whole-cell labeling.
Academic Article	Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance.
Academic Article	Fatty acylation promotes fusion of transport vesicles with Golgi cisternae.
Academic Article	A role for GTP-binding proteins in vesicular transport through the Golgi complex.
Academic Article	Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.
Academic Article	A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.
Academic Article	Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization.
Academic Article	Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
Academic Article	Journeys through the Golgi--taking stock in a new era.
Academic Article	Chromophore formation in DsRed occurs by a branched pathway.
Academic Article	Models for Golgi traffic: a critical assessment.
Academic Article	Sec12 binds to Sec16 at transitional ER sites.
Academic Article	Components responsible for transport between successive Golgi cisternae are highly conserved in evolution.
Academic Article	Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
Academic Article	Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
Academic Article	Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae.
Academic Article	The protein import receptor of mitochondria.
Academic Article	Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.
Academic Article	The yeast Golgi apparatus: insights and mysteries.
Academic Article	The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.
Academic Article	A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
Academic Article	Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
Academic Article	Noncytotoxic DsRed derivatives for whole-cell labeling.
Academic Article	Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.
Academic Article	The budding yeast Pichia pastoris has a novel Sec23p homolog.
Academic Article	Cyclophilin catalyzes protein folding in yeast mitochondria.
Academic Article	Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria.
Academic Article	Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism.
Academic Article	Fluorescence microscopy and thin-section electron microscopy.
Academic Article	A C-terminally-anchored Golgi protein is inserted into the endoplasmic reticulum and then transported to the Golgi apparatus.
Academic Article	Protein import into mitochondria: the requirement for external ATP is precursor-specific whereas intramitochondrial ATP is universally needed for translocation into the matrix.
Academic Article	A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability.
Academic Article	Import of cytochrome b2 to the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent upon matrix ATP.
Academic Article	Mutation of SYNE-1, encoding an essential component of the nuclear lamina, is responsible for autosomal recessive arthrogryposis.
Academic Article	Membrane traffic within the Golgi apparatus.
Academic Article	Organelle structure and biogenesis.
Academic Article	Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
Academic Article	Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES.
Academic Article	Fusion proteins containing the cytochrome b2 presequence are sorted to the mitochondrial intermembrane space independently of hsp60.
Academic Article	Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Academic Article	Tagging Hansenula polymorpha genes by random integration of linear DNA fragments (RALF).
Academic Article	Imaging pancreatic beta-cells in the intact pancreas.
Academic Article	Identification of pexophagy genes by restriction enzyme-mediated integration.
Academic Article	Possible role for fatty acyl-coenzyme A in intracellular protein transport.
Academic Article	Can Hsp70 proteins act as force-generating motors?
Academic Article	Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.
Academic Article	Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting.
Academic Article	Sec16 influences transitional ER sites by regulating rather than organizing COPII.
Academic Article	A three-stage model of Golgi structure and function.
Academic Article	Integrated self-organization of transitional ER and early Golgi compartments.
Academic Article	Golgi enlargement in Arf-depleted yeast cells is due to altered dynamics of cisternal maturation.
Academic Article	GenoLIB: a database of biological parts derived from a library of common plasmid features.
Academic Article	Gottfried Schatz (1936-2015)-mitochondrial pioneer and ambassador for science.
Academic Article	Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells.
Academic Article	COPI selectively drives maturation of the early Golgi.
Academic Article	The Atg17-Atg31-Atg29 Complex Coordinates with Atg11 to Recruit the Vam7 SNARE and Mediate Autophagosome-Vacuole Fusion.
Academic Article	Protein import into mitochondria: two systems acting in tandem?
Academic Article	4D Confocal Imaging of Yeast Organelles.
Academic Article	Refined Pichia pastoris reference genome sequence.
Academic Article	An improved reversibly dimerizing mutant of the FK506-binding protein FKBP.
Academic Article	New insights into protein secretion: TANGO1 runs rings around the COPII coat.
Academic Article	Budding Yeast Has a Minimal Endomembrane System.
Academic Article	Visualizing Secretory Cargo Transport in Budding Yeast.
Academic Article	Improved deconvolution of very weak confocal signals.
Academic Article	An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.
Academic Article	Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Academic Article	4D Microscopy of Yeast.
Academic Article	ER arrival sites associate with ER exit sites to create bidirectional transport portals.
Academic Article	A photostable monomeric superfolder green fluorescent protein.
Academic Article	A microscopy-based kinetic analysis of yeast vacuolar protein sorting.
Academic Article	Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.
Academic Article	ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Academic Article	Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway.
Academic Article	TRAPP structures reveal the big picture.
Academic Article	A General Method to Improve Fluorophores Using Deuterated Auxochromes.
Academic Article	Activity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome.
Academic Article	Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.
Concept	SEC Translocation Channels
Grant	The Transitional ER-Golgi System in Budding Yeasts
Grant	Optimizing and Analyzing Fluorescent Proteins
Grant	Molecular and Cellular Biology Training
Grant	Electron Microscope for a Multi-User Core Facility
Grant	Role of the AP-1 Adaptor in Intra-Golgi Recycling
Grant	Dissecting the functions of yeast COPI
Grant	2007 Molecular Membrane Biology GRC
Academic Article	Yellow and oxidation-resistant derivatives of a monomeric superfolder GFP.
Academic Article	Yellow and oxidation-resistant derivatives of a monomeric superfolder GFP.

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National Institute of Allergy and Infectious Diseases
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