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One or more keywords matched the following properties of Glick, Benjamin
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overview Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.
One or more keywords matched the following items that are connected to Glick, Benjamin
Item TypeName
Concept Saccharomyces cerevisiae Proteins
Concept Saccharomyces cerevisiae
Academic Article Hsp60-independent protein folding in the matrix of yeast mitochondria.
Academic Article Isolation of highly purified mitochondria from Saccharomyces cerevisiae.
Academic Article Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site.
Academic Article The mitochondrial protein import motor: dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis.
Academic Article Pathways and energetics of mitochondrial protein import in Saccharomyces cerevisiae.
Academic Article A yeast t-SNARE involved in endocytosis.
Academic Article Active unfolding of precursor proteins during mitochondrial protein import.
Academic Article Saccharomyces cerevisiae mitochondria lack a bacterial-type sec machinery.
Academic Article What is the driving force for protein import into mitochondria?
Academic Article Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.
Academic Article Barreling through the outer membrane.
Academic Article Isolation of Pichia pastoris genes involved in ER-to-Golgi transport.
Academic Article A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris.
Academic Article A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
Academic Article Can the Golgi form de novo?
Academic Article Monitoring changes in the subcellular location of proteins in S. cerevisiae.
Academic Article The transitional ER localization mechanism of Pichia pastoris Sec12.
Academic Article Vector for pop-in/pop-out gene replacement in Pichia pastoris.
Academic Article Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed).
Academic Article The mechanisms of vesicle budding and fusion.
Academic Article Sec16 is a determinant of transitional ER organization.
Academic Article Golgi maturation visualized in living yeast.
Academic Article Protein import into isolated yeast mitochondria.
Academic Article Cdc1p is an endoplasmic reticulum-localized putative lipid phosphatase that affects Golgi inheritance and actin polarization by activating Ca2+ signaling.
Academic Article Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance.
Academic Article Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.
Academic Article Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
Academic Article The protein import receptor of mitochondria.
Academic Article Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.
Academic Article The yeast Golgi apparatus: insights and mysteries.
Academic Article The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.
Academic Article Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.
Academic Article The budding yeast Pichia pastoris has a novel Sec23p homolog.
Academic Article Cyclophilin catalyzes protein folding in yeast mitochondria.
Academic Article Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria.
Academic Article Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism.
Academic Article A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability.
Academic Article Import of cytochrome b2 to the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent upon matrix ATP.
Academic Article Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES.
Academic Article Fusion proteins containing the cytochrome b2 presequence are sorted to the mitochondrial intermembrane space independently of hsp60.
Academic Article Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Academic Article Identification of pexophagy genes by restriction enzyme-mediated integration.
Academic Article Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.
Academic Article Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting.
Academic Article Golgi enlargement in Arf-depleted yeast cells is due to altered dynamics of cisternal maturation.
Academic Article COPI selectively drives maturation of the early Golgi.
Academic Article The Atg17-Atg31-Atg29 Complex Coordinates with Atg11 to Recruit the Vam7 SNARE and Mediate Autophagosome-Vacuole Fusion.
Academic Article 4D Confocal Imaging of Yeast Organelles.
Academic Article Visualizing Secretory Cargo Transport in Budding Yeast.
Academic Article Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Academic Article A microscopy-based kinetic analysis of yeast vacuolar protein sorting.
Academic Article Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.
Academic Article ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Academic Article TRAPP structures reveal the big picture.
Academic Article Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.
Grant The Transitional ER-Golgi System in Budding Yeasts
Grant Dissecting the functions of yeast COPI
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  • Saccharomyces