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Search Results to Douglas K. Bishop

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One or more keywords matched the following properties of Bishop, Douglas K.

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keywords meiosis, homologous recombination, homology-directed DNA repair, gene targeting, Rad51, Dmc1, RecA
overview Homologous recombination repairs DNA damage, facilitates DNA replication, and creates the physical connections between chromosomes needed for reductional chromosome segregation during meiosis. We study two key recombination proteins, Dmc1 and Rad51 that are related to the central bacterial recombination protein, RecA. The mechanisms of recombinational repair of damage induced double strand breaks in DNA (DSBs) and the mechanism of meiotic recombination are very closely related in terms of the DNA intermediates that form; DSBs are normal intermediates in most or all meiotic recombination. There are, however critical differences in how meiotic recombination is regulated as compared to mitotic recombinational repair. Our research is directed at understanding how Dmc1's function is specialized for meiosis, how the functions of Rad51 and Dmc1 differ, how the two proteins interact with one another during meiosis, and how the two proteins interact with components of the synaptonemal complex, a meiosis-specific chromosome scaffold. Our studies show that Rad51, which serves as the central “recombinase” in mitotic cells, is converted to a Dmc1 accessory protein in meiosis. Our current approaches to understanding Rad51 and Dmc1 function include biochemical reconstitution of Dmc1 activity. This work currently involves 7 meiotic recombination proteins. We were first to show that recombination proteins can be detected at multiple subnuclear sites during recombination using immunostaining techniques. We have used cytological methods in the past to identify proteins required for recruitment of recombinase to double strand break sites in mitotic and meiotic cells. These regulators include the breast cancer susceptibility genes BRCA1 and BRCA2, which promote the assembly of recombination complexes, and RAD54 family translocases, which promote disassembly. We recently began to focus our cytological efforts on super-resolution light microscopy methods including STORM and STED. These methods are shedding new light on the architecture of the recombinosome. Finally we use molecular genetic techniques that allow detection of DNA recombination intermediates to study the mechanism that regulate meiotic recombination in living cells.

One or more keywords matched the following items that are connected to Bishop, Douglas K.

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Concept Meiosis
Academic Article Saccharomyces cerevisiae checkpoint genes MEC1, RAD17 and RAD24 are required for normal meiotic recombination partner choice.
Academic Article Early decision; meiotic crossover interference prior to stable strand exchange and synapsis.
Academic Article Red-Hed regulation: recombinase Rad51, though capable of playing the leading role, may be relegated to supporting Dmc1 in budding yeast meiosis.
Academic Article The Mei5-Sae3 protein complex mediates Dmc1 activity in Saccharomyces cerevisiae.
Academic Article Tid1/Rdh54 promotes dissociation of Dmc1 from nonrecombinogenic sites on meiotic chromatin.
Academic Article Rad51, the lead in mitotic recombinational DNA repair, plays a supporting role in budding yeast meiosis.
Academic Article Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis.
Academic Article Multiple mechanisms of meiotic recombination.
Academic Article Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes.
Academic Article High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway.
Academic Article Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis.
Academic Article Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation.
Academic Article Synthesis-dependent strand annealing in meiosis.
Academic Article Gradual implementation of the meiotic recombination program via checkpoint pathways controlled by global DSB levels.
Academic Article DNA strand exchange and RecA homologs in meiosis.
Academic Article Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation.
Academic Article The third exon of the budding yeast meiotic recombination gene HOP2 is required for calcium-dependent and recombinase Dmc1-specific stimulation of homologous strand assimilation.
Academic Article Small Rad51 and Dmc1 Complexes Often Co-occupy Both Ends of a Meiotic DNA Double Strand Break.
Academic Article RPA resolves conflicting activities of accessory proteins during reconstitution of Dmc1-mediated meiotic recombination.
Academic Article Distinct Functions in Regulation of Meiotic Crossovers for DNA Damage Response Clamp Loader Rad24(Rad17) and Mec1(ATR) Kinase.
Academic Article A mutant form of Dmc1 that bypasses the requirement for accessory protein Mei5-Sae3 reveals independent activities of Mei5-Sae3 and Rad51 in Dmc1 filament stability.
Academic Article Meiosis in Quarantine discussions lead to an action plan to increase diversity and inclusion within the genetics community.

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  • Meiosis