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One or more keywords matched the following properties of Bishop, Douglas K.
overview Homologous recombination repairs DNA damage, facilitates DNA replication, and creates the physical connections between chromosomes needed for reductional chromosome segregation during meiosis. We study two key recombination proteins, Dmc1 and Rad51 that are related to the central bacterial recombination protein, RecA. The mechanisms of recombinational repair of damage induced double strand breaks in DNA (DSBs) and the mechanism of meiotic recombination are very closely related in terms of the DNA intermediates that form; DSBs are normal intermediates in most or all meiotic recombination. There are, however critical differences in how meiotic recombination is regulated as compared to mitotic recombinational repair. Our research is directed at understanding how Dmc1's function is specialized for meiosis, how the functions of Rad51 and Dmc1 differ, how the two proteins interact with one another during meiosis, and how the two proteins interact with components of the synaptonemal complex, a meiosis-specific chromosome scaffold. Our studies show that Rad51, which serves as the central “recombinase” in mitotic cells, is converted to a Dmc1 accessory protein in meiosis. Our current approaches to understanding Rad51 and Dmc1 function include biochemical reconstitution of Dmc1 activity. This work currently involves 7 meiotic recombination proteins. We were first to show that recombination proteins can be detected at multiple subnuclear sites during recombination using immunostaining techniques. We have used cytological methods in the past to identify proteins required for recruitment of recombinase to double strand break sites in mitotic and meiotic cells. These regulators include the breast cancer susceptibility genes BRCA1 and BRCA2, which promote the assembly of recombination complexes, and RAD54 family translocases, which promote disassembly. We recently began to focus our cytological efforts on super-resolution light microscopy methods including STORM and STED. These methods are shedding new light on the architecture of the recombinosome. Finally we use molecular genetic techniques that allow detection of DNA recombination intermediates to study the mechanism that regulate meiotic recombination in living cells.
One or more keywords matched the following items that are connected to Bishop, Douglas K.
Item TypeName
Concept Rec A Recombinases
Concept Rad51 Recombinase
Concept Recombinases
Academic Article Xrcc3 is required for assembly of Rad51 complexes in vivo.
Academic Article BRCA2 and homologous recombination.
Academic Article The breast cancer susceptibility gene BRCA1 is required for subnuclear assembly of Rad51 and survival following treatment with the DNA cross-linking agent cisplatin.
Academic Article Red-Hed regulation: recombinase Rad51, though capable of playing the leading role, may be relegated to supporting Dmc1 in budding yeast meiosis.
Academic Article RAD51 up-regulation bypasses BRCA1 function and is a common feature of BRCA1-deficient breast tumors.
Academic Article A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments.
Academic Article The Mei5-Sae3 protein complex mediates Dmc1 activity in Saccharomyces cerevisiae.
Academic Article Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth.
Academic Article The epistatic relationship between BRCA2 and the other RAD51 mediators in homologous recombination.
Academic Article Rad51, the lead in mitotic recombinational DNA repair, plays a supporting role in budding yeast meiosis.
Academic Article A chemical compound that stimulates the human homologous recombination protein RAD51.
Academic Article Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis.
Academic Article RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells.
Academic Article RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis.
Academic Article Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes.
Academic Article High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway.
Academic Article Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis.
Academic Article RecA homology search is promoted by mechanical stress along the scanned duplex DNA.
Academic Article Real-time solution measurement of RAD51- and RecA-mediated strand assimilation without background annealing.
Academic Article Gradual implementation of the meiotic recombination program via checkpoint pathways controlled by global DSB levels.
Academic Article DNA strand exchange and RecA homologs in meiosis.
Academic Article RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.
Academic Article Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation.
Academic Article The third exon of the budding yeast meiotic recombination gene HOP2 is required for calcium-dependent and recombinase Dmc1-specific stimulation of homologous strand assimilation.
Academic Article The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors.
Academic Article Caffeine impairs resection during DNA break repair by reducing the levels of nucleases Sae2 and Dna2.
Academic Article Caffeine inhibits gene conversion by displacing Rad51 from ssDNA.
Academic Article Small Rad51 and Dmc1 Complexes Often Co-occupy Both Ends of a Meiotic DNA Double Strand Break.
Academic Article The ATPase activity of E. coli RecA prevents accumulation of toxic complexes formed by erroneous binding to undamaged double stranded DNA.
Academic Article RPA resolves conflicting activities of accessory proteins during reconstitution of Dmc1-mediated meiotic recombination.
Academic Article Non-enzymatic roles of human RAD51 at stalled replication forks.
Academic Article A mutant form of Dmc1 that bypasses the requirement for accessory protein Mei5-Sae3 reveals independent activities of Mei5-Sae3 and Rad51 in Dmc1 filament stability.
Academic Article How strand exchange protein function benefits from ATP hydrolysis.
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  • Recombinases