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David Pincus

TitleAssistant Professor
InstitutionUniversity of Chicago
DepartmentMolecular Genetics and Cell Biology
AddressChicago IL 60637
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    Collapse Overview 
    Collapse overview
    David Pincus is an Assistant Professor in the Dept. of Molecular Genetics and Cell Biology. The Pincus lab is located in the Center for Physics of Evolving Systems on the 5th floor of GCIS.

    The Pincus Lab studies cellular adaptation at three levels: cell biological mechanisms of adaptation to environmental stress, global principles of adaptation and resource allocation in complex environments, and the intersection of physiological stress response factors and evolutionary adaptation.

    David is trained in approaches in biochemistry, biophysics, genetics, genomics, and molecular, cell, computational, systems and synthetic biology. The lab uses budding yeast and cultured human cells as experimental models.

    Key project areas:
    1) Quantitative cell biology of the heat shock response
    2) Single-cell transcriptomics in complex stress environments
    Collapse webpage

    Collapse Biography 
    Collapse education and training
    UC Berkeley, Berkeley, CABA12/2004Molecular & Cell Biology
    UCSF, San Francisco, CAPhD09/2012Biochemistry
    Whitehead Institute, Cambridge, MA09/2018
    Collapse awards and honors
    2007 - 2010Graduate Research Fellowship, NSF
    2013 - 2015Stewart Trust Cancer Fellowship, Alexander and Margaret Stewart Trust
    2013 - 2018Early Independence Award (DP5), NIH Office of the Director

    Collapse Research 
    Collapse research activities and funding
    R01GM138689     (PINCUS, DAVID)Apr 13, 2021 - Mar 31, 2025
    Regulatory Dynamics of the Proteostasis Network
    Role: Principal Investigator

    DP5OD017941     (PINCUS, DAVID)Sep 19, 2013 - Sep 30, 2018
    Quantitative approaches to reveal the homeostatic control mechanisms of stress re
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Garde R, Dea A, Herwig MF, Pincus D. Feedback control of the heat shock response by spatiotemporal regulation of Hsp70. bioRxiv. 2024 Jan 09. PMID: 38260373; PMCID: PMC10802473.
    2. Ali A, Garde R, Schaffer OC, Bard JAM, Husain K, Kik SK, Davis KA, Luengo-Woods S, Igarashi MG, Drummond DA, Squires AH, Pincus D. Adaptive preservation of orphan ribosomal proteins in chaperone-dispersed condensates. Nat Cell Biol. 2023 Nov; 25(11):1691-1703. PMID: 37845327; PMCID: PMC10868727.
      Citations: 2     Fields:    Translation:HumansAnimalsCells
    3. Gao J, Zhang R, Zheng L, Song L, Ji M, Li S, Wang J, Yang J, Kang G, Zhang P, Shi Y, Jiao Y, Pincus D, Zheng X. Blue light receptor CRY1 regulates HSFA1d nuclear localization to promote plant thermotolerance. Cell Rep. 2023 Sep 26; 42(9):113117. PMID: 37703177; PMCID: PMC10591714.
      Citations:    Fields:    
    4. Garde R, Singh A, Ali A, Pincus D. Transcriptional regulation of Sis1 promotes fitness but not feedback in the heat shock response. Elife. 2023 05 09; 12. PMID: 37158601; PMCID: PMC10191621.
      Citations: 3     Fields:    Translation:AnimalsCells
    5. Chowdhary S, Kainth AS, Paracha S, Gross DS, Pincus D. Inducible transcriptional condensates drive 3D genome reorganization in the heat shock response. Mol Cell. 2022 11 17; 82(22):4386-4399.e7. PMID: 36327976; PMCID: PMC9701134.
      Citations: 12     Fields:    Translation:AnimalsCells
    6. Kainth AS, Chowdhary S, Pincus D, Gross DS. Primordial super-enhancers: heat shock-induced chromatin organization in yeast. Trends Cell Biol. 2021 10; 31(10):801-813. PMID: 34001402; PMCID: PMC8448919.
      Citations: 12     Fields:    Translation:AnimalsCells
    7. Ali A, Singh A, Krakowiak J, Zheng X, Bindokas VP, Wolfgeher D, Kron SJ, Pincus D, Feder ZA. Subcellular localization of the J-protein Sis1 regulates the heat shock response. J Cell Biol. 2021 01 04; 220(1). PMID: 33326013; PMCID: PMC7748816.
      Citations: 12     Fields:    Translation:AnimalsCells
    8. Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 Sep 08; 32(10):108149. PMID: 32905780.
      Citations:    Fields:    
    9. Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 08 11; 32(6):108001. PMID: 32783929; PMCID: PMC10088179.
      Citations: 1     Fields:    Translation:HumansCells
    10. Mace K, Krakowiak J, El-Samad H, Pincus D. Multi-kinase control of environmental stress responsive transcription. PLoS One. 2020; 15(3):e0230246. PMID: 32160258; PMCID: PMC7065805.
      Citations: 8     Fields:    Translation:AnimalsCells
    11. Pincus D. Regulation of Hsf1 and the Heat Shock Response. Adv Exp Med Biol. 2020; 1243:41-50. PMID: 32297210.
      Citations: 34     Fields:    Translation:HumansAnimalsCells
    12. McCormick JW, Pincus D, Resnekov O, Reynolds KA. Strategies for Engineering and Rewiring Kinase Regulation. Trends Biochem Sci. 2020 03; 45(3):259-271. PMID: 31866305.
      Citations: 10     Fields:    Translation:HumansAnimalsCells
    13. Bushkin GG, Pincus D, Morgan JT, Richardson K, Lewis C, Chan SH, Bartel DP, Fink GR. m6A modification of a 3' UTR site reduces RME1 mRNA levels to promote meiosis. Nat Commun. 2019 07 30; 10(1):3414. PMID: 31363087; PMCID: PMC6667471.
      Citations: 27     Fields:    Translation:AnimalsCells
    14. Tye BW, Commins N, Ryazanova LV, Springer M, Pincus D, Churchman LS, Wühr M. Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness. Elife. 2019 03 07; 8. PMID: 30843788; PMCID: PMC6453566.
      Citations: 52     Fields:    Translation:AnimalsCells
    15. Chowdhary S, Kainth AS, Pincus D, Gross DS. Heat Shock Factor 1 Drives Intergenic Association of Its Target Gene Loci upon Heat Shock. Cell Rep. 2019 01 02; 26(1):18-28.e5. PMID: 30605674; PMCID: PMC6435272.
      Citations: 28     Fields:    Translation:Animals
    16. Fanning S, Haque A, Imberdis T, Baru V, Barrasa MI, Nuber S, Termine D, Ramalingam N, Ho GPH, Noble T, Sandoe J, Lou Y, Landgraf D, Freyzon Y, Newby G, Soldner F, Terry-Kantor E, Kim TE, Hofbauer HF, Becuwe M, Jaenisch R, Pincus D, Clish CB, Walther TC, Farese RV, Srinivasan S, Welte MA, Kohlwein SD, Dettmer U, Lindquist S, Selkoe D. Lipidomic Analysis of a-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 2019 03 07; 73(5):1001-1014.e8. PMID: 30527540; PMCID: PMC6408259.
      Citations: 118     Fields:    Translation:HumansAnimalsCells
    17. Pincus D, Pandey JP, Creixell P, Resnekov O, Reynolds KA, Feder ZA. Engineering allosteric regulation in protein kinases. Sci Signal. 2018 11 06; 11(555). PMID: 30401787; PMCID: PMC6662207.
      Citations: 10     Fields:    Translation:AnimalsCells
    18. Pincus D, Anandhakumar J, Thiru P, Guertin MJ, Erkine AM, Gross DS. Genetic and epigenetic determinants establish a continuum of Hsf1 occupancy and activity across the yeast genome. Mol Biol Cell. 2018 12 15; 29(26):3168-3182. PMID: 30332327; PMCID: PMC6340206.
      Citations: 31     Fields:    Translation:AnimalsCells
    19. Creixell P, Pandey JP, Palmeri A, Bhattacharyya M, Creixell M, Ranganathan R, Pincus D, Yaffe MB. Hierarchical Organization Endows the Kinase Domain with Regulatory Plasticity. Cell Syst. 2018 10 24; 7(4):371-383.e4. PMID: 30243563; PMCID: PMC6202253.
      Citations: 9     Fields:    Translation:HumansCells
    20. Truttmann MC, Pincus D, Ploegh HL. Chaperone AMPylation modulates aggregation and toxicity of neurodegenerative disease-associated polypeptides. Proc Natl Acad Sci U S A. 2018 05 29; 115(22):E5008-E5017. PMID: 29760078; PMCID: PMC5984528.
      Citations: 15     Fields:    Translation:AnimalsCells
    21. Zheng X, Beyzavi A, Krakowiak J, Patel N, Khalil AS, Pincus D. Hsf1 Phosphorylation Generates Cell-to-Cell Variation in Hsp90 Levels and Promotes Phenotypic Plasticity. Cell Rep. 2018 03 20; 22(12):3099-3106. PMID: 29562166; PMCID: PMC5893160.
      Citations: 17     Fields:    Translation:AnimalsCells
    22. Kayatekin C, Amasino A, Gaglia G, Flannick J, Bonner JM, Fanning S, Narayan P, Barrasa MI, Pincus D, Landgraf D, Nelson J, Hesse WR, Costanzo M, AMP T2D-GENES Consortium, Myers CL, Boone C, Florez JC, Lindquist S. Translocon Declogger Ste24 Protects against IAPP Oligomer-Induced Proteotoxicity. Cell. 2018 03 22; 173(1):62-73.e9. PMID: 29526462; PMCID: PMC5945206.
      Citations: 29     Fields:    Translation:HumansAnimalsCells
    23. Krakowiak J, Zheng X, Patel N, Anandhakumar J, Valerius K, Gross DS, Khalil AS, Pincus D, Feder ZA. Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response. Elife. 2018 02 02; 7. PMID: 29393852; PMCID: PMC5809143.
      Citations: 50     Fields:    Translation:Animals
    24. Pandey JP, Zheng X, Jin DX, Gupta PB, Airoldi EM, Pincus D, Denic V, Solís EJ. Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis. Mol Cell. 2018 02 01; 69(3):534. PMID: 29395071; PMCID: PMC5813686.
      Citations: 4     Fields:    
    25. Zheng X, Pincus D. Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins. Bio Protoc. 2017 Jun 20; 7(12). PMID: 29104895; PMCID: PMC5669396.
      Citations: 4     
    26. Pincus D, Resnekov O, Reynolds KA. An evolution-based strategy for engineering allosteric regulation. Phys Biol. 2017 04 28; 14(2):025002. PMID: 28266924; PMCID: PMC5943710.
      Citations: 5     Fields:    Translation:Cells
    27. Truttmann MC, Zheng X, Hanke L, Damon JR, Grootveld M, Krakowiak J, Pincus D, Ploegh HL. Unrestrained AMPylation targets cytosolic chaperones and activates the heat shock response. Proc Natl Acad Sci U S A. 2017 01 10; 114(2):E152-E160. PMID: 28031489; PMCID: PMC5240723.
      Citations: 15     Fields:    Translation:HumansAnimalsCells
    28. Zheng X, Krakowiak J, Patel N, Beyzavi A, Ezike J, Khalil AS, Pincus D. Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation. Elife. 2016 11 10; 5. PMID: 27831465; PMCID: PMC5127643.
      Citations: 99     Fields:    Translation:AnimalsCells
    29. Pincus D. Size doesn't matter in the heat shock response. Curr Genet. 2017 May; 63(2):175-178. PMID: 27502399; PMCID: PMC5298949.
      Citations: 3     Fields:    Translation:Animals
    30. Pandey JP, Zheng X, Jin DX, Gupta PB, Airoldi EM, Pincus D, Denic V, Solís EJ. Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis. Mol Cell. 2016 07 07; 63(1):60-71. PMID: 27320198; PMCID: PMC4938784.
      Citations: 91     Fields:    Translation:AnimalsCells
    31. van Anken E, Pincus D, Coyle S, Osman C, Lari F, Korennykh AV, Walter P, Aragón T, Gómez Puerta S. Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1. Elife. 2014 Dec 30; 3:e05031. PMID: 25549299; PMCID: PMC4279078.
      Citations: 21     Fields:    Translation:AnimalsCells
    32. Damon JR, Pincus D, Ploegh HL. tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae. Mol Biol Cell. 2015 Jan 15; 26(2):270-82. PMID: 25392298; PMCID: PMC4294674.
      Citations: 38     Fields:    Translation:AnimalsCells
    33. Pincus D, Ryan CJ, Smith RD, Brent R, Resnekov O. Assigning quantitative function to post-translational modifications reveals multiple sites of phosphorylation that tune yeast pheromone signaling output. PLoS One. 2013; 8(3):e56544. PMID: 23554854; PMCID: PMC3595240.
      Citations: 1     Fields:    Translation:AnimalsCells
    34. Vidal SE, Pincus D, Stewart-Ornstein J, El-Samad H. Formation of subnuclear foci is a unique spatial behavior of mating MAPKs during hyperosmotic stress. Cell Rep. 2013 Feb 21; 3(2):328-34. PMID: 23416049; PMCID: PMC3616637.
      Citations: 2     Fields:    Translation:AnimalsCells
    35. Fordyce PM, Pincus D, Kimmig P, Nelson CS, El-Samad H, Walter P, DeRisi JL. Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses. Proc Natl Acad Sci U S A. 2012 Nov 06; 109(45):E3084-93. PMID: 23054834; PMCID: PMC3494901.
      Citations: 34     Fields:    Translation:AnimalsCells
    36. Thomson TM, Benjamin KR, Bush A, Love T, Pincus D, Resnekov O, Yu RC, Gordon A, Colman-Lerner A, Endy D, Brent R. Scaffold number in yeast signaling system sets tradeoff between system output and dynamic range. Proc Natl Acad Sci U S A. 2011 Dec 13; 108(50):20265-70. PMID: 22114196; PMCID: PMC3250143.
      Citations: 31     Fields:    Translation:AnimalsCells
    37. Pincus D, Chevalier MW, van Anken E, Vidal SE, El-Samad H, Walter P, Aragón T. BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response. PLoS Biol. 2010 Jul 06; 8(7):e1000415. PMID: 20625545; PMCID: PMC2897766.
      Citations: 219     Fields:    Translation:AnimalsCells
    38. Pincus D, Benjamin K, Burbulis I, Tsong AE, Resnekov O. Reagents for investigating MAPK signalling in model yeast species. Yeast. 2010 Jul; 27(7):423-30. PMID: 20162536.
      Citations: 4     Fields:    Translation:AnimalsCells
    39. van Anken E, Pincus D, Serafimova IM, Korennykh AV, Rubio CA, Walter P, Aragón T. Messenger RNA targeting to endoplasmic reticulum stress signalling sites. Nature. 2009 Feb 05; 457(7230):736-40. PMID: 19079237; PMCID: PMC2768538.
      Citations: 163     Fields:    Translation:AnimalsCells
    40. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Negative feedback that improves information transmission in yeast signalling. Nature. 2008 Dec 11; 456(7223):755-61. PMID: 19079053; PMCID: PMC2716709.
      Citations: 113     Fields:    Translation:AnimalsCells
    41. King N, Westbrook MJ, Young SL, Kuo A, Abedin M, Chapman J, Fairclough S, Hellsten U, Isogai Y, Letunic I, Marr M, Pincus D, Putnam N, Rokas A, Wright KJ, Zuzow R, Dirks W, Good M, Goodstein D, Lemons D, Li W, Lyons JB, Morris A, Nichols S, Richter DJ, Salamov A, Sequencing JG, Bork P, Lim WA, Manning G, Miller WT, McGinnis W, Shapiro H, Tjian R, Grigoriev IV, Rokhsar D. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature. 2008 Feb 14; 451(7180):783-8. PMID: 18273011; PMCID: PMC2562698.
      Citations: 487     Fields:    Translation:HumansAnimalsCells
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