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David Pincus

AddressChicago IL 60637
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    Collapse Overview 
    Collapse overview
    David Pincus is an Assistant Professor in the Dept. of Molecular Genetics and Cell Biology. The Pincus lab is located in the Center for Physics of Evolving Systems on the 5th floor of GCIS.

    The Pincus Lab studies "System Mechanics of Cellular Stress Responses", defined as quantitative control processes cells employ to sense and respond to environmental and endogenous perturbations.

    David is trained in approaches in biochemistry, biophysics, genetics, and molecular, cell, computational, systems and synthetic biology. The lab uses budding yeast and cultured human cells as experimental models, and applies this toolkit to investigate three interconnected areas:

    1) Hsf1, chaperones, and proteostasis networks
    2) Stress responsive kinase signaling
    3) Stress responsive gene expression

    The goal of the research program is to understand the establishment and maintenance of cell-level homeostasis.


    Collapse Biography 
    Collapse education and training
    UC Berkeley, Berkeley, CABA12/2004Molecular & Cell Biology
    UCSF, San Francisco, CAPhD09/2012Biochemistry
    Whitehead Institute, Cambridge, MA09/2018
    Collapse awards and honors
    2007 - 2010Graduate Research Felloship, NSF
    2013 - 2015Stewart Trust Cancer Fellowship, Alexander and Margaret Stewart Trust
    2013 - 2018Early Independence Award (DP5), NIH Office of the Director

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 Sep 08; 32(10):108149. PMID: 32905780.
      View in: PubMed
    2. Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 Aug 11; 32(6):108001. PMID: 32783929.
      View in: PubMed
    3. Mace K, Krakowiak J, El-Samad H, Pincus D. Multi-kinase control of environmental stress responsive transcription. PLoS One. 2020; 15(3):e0230246. PMID: 32160258.
      View in: PubMed
    4. Pincus D. Regulation of Hsf1 and the Heat Shock Response. Adv Exp Med Biol. 2020; 1243:41-50. PMID: 32297210.
      View in: PubMed
    5. McCormick JW, Pincus D, Resnekov O, Reynolds KA. Strategies for Engineering and Rewiring Kinase Regulation. Trends Biochem Sci. 2020 Mar; 45(3):259-271. PMID: 31866305.
      View in: PubMed
    6. Bushkin GG, Pincus D, Morgan JT, Richardson K, Lewis C, Chan SH, Bartel DP, Fink GR. m6A modification of a 3' UTR site reduces RME1 mRNA levels to promote meiosis. Nat Commun. 2019 07 30; 10(1):3414. PMID: 31363087.
      View in: PubMed
    7. Tye BW, Commins N, Ryazanova LV, Wühr M, Springer M, Pincus D, Churchman LS. Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness. Elife. 2019 03 07; 8. PMID: 30843788.
      View in: PubMed
    8. Chowdhary S, Kainth AS, Pincus D, Gross DS. Heat Shock Factor 1 Drives Intergenic Association of Its Target Gene Loci upon Heat Shock. Cell Rep. 2019 01 02; 26(1):18-28.e5. PMID: 30605674.
      View in: PubMed
    9. Fanning S, Haque A, Imberdis T, Baru V, Barrasa MI, Nuber S, Termine D, Ramalingam N, Ho GPH, Noble T, Sandoe J, Lou Y, Landgraf D, Freyzon Y, Newby G, Soldner F, Terry-Kantor E, Kim TE, Hofbauer HF, Becuwe M, Jaenisch R, Pincus D, Clish CB, Walther TC, Farese RV, Srinivasan S, Welte MA, Kohlwein SD, Dettmer U, Lindquist S, Selkoe D. Lipidomic Analysis of a-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 2019 03 07; 73(5):1001-1014.e8. PMID: 30527540.
      View in: PubMed
    10. Pincus D, Pandey JP, Feder ZA, Creixell P, Resnekov O, Reynolds KA. Engineering allosteric regulation in protein kinases. Sci Signal. 2018 11 06; 11(555). PMID: 30401787.
      View in: PubMed
    11. Pincus D, Anandhakumar J, Thiru P, Guertin MJ, Erkine AM, Gross DS. Genetic and epigenetic determinants establish a continuum of Hsf1 occupancy and activity across the yeast genome. Mol Biol Cell. 2018 12 15; 29(26):3168-3182. PMID: 30332327.
      View in: PubMed
    12. Creixell P, Pandey JP, Palmeri A, Bhattacharyya M, Creixell M, Ranganathan R, Pincus D, Yaffe MB. Hierarchical Organization Endows the Kinase Domain with Regulatory Plasticity. Cell Syst. 2018 10 24; 7(4):371-383.e4. PMID: 30243563.
      View in: PubMed
    13. Truttmann MC, Pincus D, Ploegh HL. Chaperone AMPylation modulates aggregation and toxicity of neurodegenerative disease-associated polypeptides. Proc Natl Acad Sci U S A. 2018 05 29; 115(22):E5008-E5017. PMID: 29760078.
      View in: PubMed
    14. Zheng X, Beyzavi A, Krakowiak J, Patel N, Khalil AS, Pincus D. Hsf1 Phosphorylation Generates Cell-to-Cell Variation in Hsp90 Levels and Promotes Phenotypic Plasticity. Cell Rep. 2018 03 20; 22(12):3099-3106. PMID: 29562166.
      View in: PubMed
    15. Kayatekin C, Amasino A, Gaglia G, Flannick J, Bonner JM, Fanning S, Narayan P, Barrasa MI, Pincus D, Landgraf D, Nelson J, Hesse WR, Costanzo M. Translocon Declogger Ste24 Protects against IAPP Oligomer-Induced Proteotoxicity. Cell. 2018 03 22; 173(1):62-73.e9. PMID: 29526462.
      View in: PubMed
    16. Krakowiak J, Zheng X, Patel N, Feder ZA, Anandhakumar J, Valerius K, Gross DS, Khalil AS, Pincus D. Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response. Elife. 2018 02 02; 7. PMID: 29393852.
      View in: PubMed
    17. Solís EJ, Pandey JP, Zheng X, Jin DX, Gupta PB, Airoldi EM, Pincus D, Denic V. Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis. Mol Cell. 2018 02 01; 69(3):534. PMID: 29395071.
      View in: PubMed
    18. Zheng X, Pincus D. Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins. Bio Protoc. 2017 Jun 20; 7(12). PMID: 29104895.
      View in: PubMed
    19. Pincus D, Resnekov O, Reynolds KA. An evolution-based strategy for engineering allosteric regulation. Phys Biol. 2017 04 28; 14(2):025002. PMID: 28266924.
      View in: PubMed
    20. Truttmann MC, Zheng X, Hanke L, Damon JR, Grootveld M, Krakowiak J, Pincus D, Ploegh HL. Unrestrained AMPylation targets cytosolic chaperones and activates the heat shock response. Proc Natl Acad Sci U S A. 2017 01 10; 114(2):E152-E160. PMID: 28031489.
      View in: PubMed
    21. Zheng X, Krakowiak J, Patel N, Beyzavi A, Ezike J, Khalil AS, Pincus D. Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation. Elife. 2016 11 10; 5. PMID: 27831465.
      View in: PubMed
    22. Pincus D. Size doesn't matter in the heat shock response. Curr Genet. 2017 May; 63(2):175-178. PMID: 27502399.
      View in: PubMed
    23. Solís EJ, Pandey JP, Zheng X, Jin DX, Gupta PB, Airoldi EM, Pincus D, Denic V. Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis. Mol Cell. 2016 07 07; 63(1):60-71. PMID: 27320198.
      View in: PubMed
    24. van Anken E, Pincus D, Coyle S, Aragón T, Osman C, Lari F, Gómez Puerta S, Korennykh AV, Walter P. Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1. Elife. 2014 Dec 30; 3:e05031. PMID: 25549299.
      View in: PubMed
    25. Damon JR, Pincus D, Ploegh HL. tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae. Mol Biol Cell. 2015 Jan 15; 26(2):270-82. PMID: 25392298.
      View in: PubMed
    26. Pincus D, Ryan CJ, Smith RD, Brent R, Resnekov O. Assigning quantitative function to post-translational modifications reveals multiple sites of phosphorylation that tune yeast pheromone signaling output. PLoS One. 2013; 8(3):e56544. PMID: 23554854.
      View in: PubMed
    27. Vidal SE, Pincus D, Stewart-Ornstein J, El-Samad H. Formation of subnuclear foci is a unique spatial behavior of mating MAPKs during hyperosmotic stress. Cell Rep. 2013 Feb 21; 3(2):328-34. PMID: 23416049.
      View in: PubMed
    28. Fordyce PM, Pincus D, Kimmig P, Nelson CS, El-Samad H, Walter P, DeRisi JL. Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses. Proc Natl Acad Sci U S A. 2012 Nov 06; 109(45):E3084-93. PMID: 23054834.
      View in: PubMed
    29. Thomson TM, Benjamin KR, Bush A, Love T, Pincus D, Resnekov O, Yu RC, Gordon A, Colman-Lerner A, Endy D, Brent R. Scaffold number in yeast signaling system sets tradeoff between system output and dynamic range. Proc Natl Acad Sci U S A. 2011 Dec 13; 108(50):20265-70. PMID: 22114196.
      View in: PubMed
    30. Pincus D, Chevalier MW, Aragón T, van Anken E, Vidal SE, El-Samad H, Walter P. BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response. PLoS Biol. 2010 Jul 06; 8(7):e1000415. PMID: 20625545.
      View in: PubMed
    31. Pincus D, Benjamin K, Burbulis I, Tsong AE, Resnekov O. Reagents for investigating MAPK signalling in model yeast species. Yeast. 2010 Jul; 27(7):423-30. PMID: 20162536.
      View in: PubMed
    32. Aragón T, van Anken E, Pincus D, Serafimova IM, Korennykh AV, Rubio CA, Walter P. Messenger RNA targeting to endoplasmic reticulum stress signalling sites. Nature. 2009 Feb 05; 457(7230):736-40. PMID: 19079237.
      View in: PubMed
    33. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Negative feedback that improves information transmission in yeast signalling. Nature. 2008 Dec 11; 456(7223):755-61. PMID: 19079053.
      View in: PubMed
    34. King N, Westbrook MJ, Young SL, Kuo A, Abedin M, Chapman J, Fairclough S, Hellsten U, Isogai Y, Letunic I, Marr M, Pincus D, Putnam N, Rokas A, Wright KJ, Zuzow R, Dirks W, Good M, Goodstein D, Lemons D, Li W, Lyons JB, Morris A, Nichols S, Richter DJ, Salamov A, Sequencing JG, Bork P, Lim WA, Manning G, Miller WT, McGinnis W, Shapiro H, Tjian R, Grigoriev IV, Rokhsar D. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature. 2008 Feb 14; 451(7180):783-8. PMID: 18273011.
      View in: PubMed
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