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Sara Szuchet

InstitutionUniversity of Chicago
AddressChicago IL 60637
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    Collapse Overview 
    Collapse overview
    The long-term goal of my laboratory is to decipher the etiology and pathogenesis of multiple sclerosis (MS) – a CNS demyelinating and neurodegenerative disease that cripples the young and bright. With the firm belief that in order to decrypt the breakdown of myelin in MS, we have to understand how it is formed, our current efforts are centered on two major projects. One, defining the molecular mechanism of myelin sheath formation by following the synthesis of myelin membranes (MyMs), their packaging into myelinophore organelles (MFOs) and transportation along an oligodendrocyte process using live imaging of embryonic avian optic nerve at different stages of development; purifying and characterizing the contents of MFOs. Two, testing the hypothesis that ectosomes are the conveyors of signals that coordinate and orchestrate the modular structure of the myelinated axon. We are developing technologies to obtain pure cell-specific ectosomes by molecularly color-code them. We are also customizing a microfluidic platform suitable to integrate multiple processes in a single lab-on-a-chip format. We plan to establish unique EM grid compatible bi-chamber for OLG-neuron cultures and capture the formation of cell-specific ectosomes using correlative light and electron microscopy and phase contrast cryo-electron tomography. We will assemble tomograms of the initial OLG-neuron encounter so that we can structurally model the interface in a 3D space.

    Collapse Biography 
    Collapse education and training
    NINDS, NIH, Maryland, USAVisiting Scientist06/2002Developmental Neurogenetics
    La Jolla Cancer Research Foundation, La Jolla, CA, USAVisiting Scientist09/1994Molecular Biology
    Department of Chemistry, Princeton University, USAPost-doctoral Fellow12/1966Nucleic Acid Chemistry
    Cambridge University, UKDoctor of Philosophy09/1963Physical Biochemistry
    University of Chile, Santiago, ChileN/A12/1959Chemistry
    Facultad of Exact Sciences, University of Buenos Aires, ArgentinaLicenciada in Chemistry12/1956Chemistry
    Collapse awards and honors
    2015 - 2016Big Vision Award, The University of Chicago
    2001 - 2002Ruth L Kirschstein National Research Service Award, NINDS, NIH
    1993 - 1994Dana Haughton Senior Fellowship, National Multiple Sclerosis Society
    1993 - 1994American Cancer Society Scholar, American Cancer Society
    1976 - 1978National Multiple Sclerosis Society, National Multiple Sclerosis Society Fellowship
    1962 - 1963Oliver Gatty Research Student, University of Cambridge, UK
    1961 - 1963Tucker-Price Research Scholar, Girton College, Cambridge, UK
    1959 - 1960Fellowship Consejo Nacional de Investigacions Cientificas y Technicas, Argentina

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Szuchet S, Nielsen LL, Domowicz MS, Austin JR, Arvanitis DL. CNS myelin sheath is stochastically built by homotypic fusion of myelin membranes within the bounds of an oligodendrocyte process. J Struct Biol. 2015 Apr; 190(1):56-72. PMID: 25682762.
      Citations: 7     Fields:    Translation:AnimalsCells
    2. Szuchet S, Nielsen JA, Lovas G, Domowicz MS, de Velasco JM, Maric D, Hudson LD. The genetic signature of perineuronal oligodendrocytes reveals their unique phenotype. Eur J Neurosci. 2011 Dec; 34(12):1906-22. PMID: 22132705; PMCID: PMC4286392.
      Citations: 14     Fields:    Translation:AnimalsCells
    3. Szuchet S, Plachetzki DC, Seeger MA, Domowicz MS, Szele FG. NOVOcan: a molecular link among selected glial cells. Biophys Chem. 2004 Mar 01; 108(1-3):245-58. PMID: 15043933.
      Citations:    Fields:    Translation:HumansAnimalsCells
    4. Szuchet S, Plachetzki DC, Karialukas R. Oligodendrocytes express an alpha/beta-interferon-susceptible Mx gene: molecular characterization of the encoded protein. Glia. 2002 Feb; 37(2):183-9. PMID: 11754216.
      Citations: 1     Fields:    Translation:AnimalsCells
    5. Szuchet S, Plachetzki DC, Eaton KS. Oligodendrocyte transmembrane protein: a novel member of the glutamate-binding protein subfamily. Biochem Biophys Res Commun. 2001 May 18; 283(4):900-7. PMID: 11350070.
      Citations: 3     Fields:    Translation:AnimalsCells
    6. Arvanitis DL, Stavridou AI, Mori de Moro G, Szuchet S, Arvanitis DL, Stavridou AI, Mori de Moro G, Szuchet S. Reactive astrocytes upregulate one or more gene products that are recognized by monoclonal antibody H. Cell Tissue Res. 2001 Apr; 304(1):11-9. PMID: 11383877.
      Citations:    Fields:    Translation:HumansAnimalsCells
    7. Szuchet S, Watanabe K, Yamaguchi Y. Differentiation/regeneration of oligodendrocytes entails the assembly of a cell-associated matrix. Int J Dev Neurosci. 2000 Nov; 18(7):705-20. PMID: 10978849.
      Citations: 2     Fields:    Translation:AnimalsCells
    8. Sanyal B, Polak PE, Szuchet S. Differential expression of the heavy-chain ferritin gene in non-adhered and adhered oligodendrocytes. J Neurosci Res. 1996 Oct 15; 46(2):187-97. PMID: 8915895.
      Citations: 3     Fields:    Translation:HumansAnimalsCells
    9. Arvanitis D, Kouklis P, Demoro G, Goutas N, Kittas C, Szuchet S. Immunostaining of ductal breast carcinomas with the monoclonal-antibody-h. Oncol Rep. 1995 Nov; 2(6):991-5. PMID: 21597840.
      Citations:    Fields:    
    10. LoPresti P, Szuchet S, Papasozomenos SC, Zinkowski RP, Binder LI. Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. Proc Natl Acad Sci U S A. 1995 Oct 24; 92(22):10369-73. PMID: 7479786; PMCID: PMC40798.
      Citations: 138     Fields:    Translation:AnimalsCells
    11. Fierro-Renoy JF, Szuchet S, Falcone M, Macchia E, DeGroot L. Three different thyroid hormone receptor isoforms are detected in a pure culture of ovine oligodendrocytes. Glia. 1995 Aug; 14(4):322-8. PMID: 8530188.
      Citations: 5     Fields:    Translation:AnimalsCells
    12. Soliven B, Szuchet S. Signal transduction pathways in oligodendrocytes: role of tumor necrosis factor-alpha. Int J Dev Neurosci. 1995 Jun-Jul; 13(3-4):351-67. PMID: 7572287.
      Citations: 4     Fields:    Translation:HumansAnimalsCells
    13. Schirmer EC, Farooqui J, Polak PE, Szuchet S. GRASP: a novel heparin-binding serum glycoprotein that mediates oligodendrocyte-substratum adhesion. J Neurosci Res. 1994 Nov 01; 39(4):457-73. PMID: 7533846.
      Citations: 1     Fields:    Translation:HumansAnimalsCells
    14. Soliven B, Takeda M, Szuchet S. Depolarizing agents and tumor necrosis factor-alpha modulate protein phosphorylation in oligodendrocytes. J Neurosci Res. 1994 May 01; 38(1):91-100. PMID: 7520088.
      Citations: 5     Fields:    Translation:AnimalsCells
    15. Ludwin SK, Szuchet S. Myelination by mature ovine oligodendrocytes in vivo and in vitro: evidence that different steps in the myelination process are independently controlled. Glia. 1993 Aug; 8(4):219-31. PMID: 8406679.
      Citations: 3     Fields:    Translation:AnimalsCells
    16. Yim SH, Sherin JE, Szuchet S. Oligodendrocyte proteoglycans: modulation by cell-substratum adhesion. J Neurosci Res. 1993 Mar 01; 34(4):401-13. PMID: 8474142.
      Citations: 4     Fields:    Translation:AnimalsCells
    17. Vartanian T, Szuchet S, Dawson G. Oligodendrocyte-substratum adhesion activates the synthesis of specific lipid species involved in cell signaling. J Neurosci Res. 1992 May; 32(1):69-78. PMID: 1321254.
      Citations: 2     Fields:    Translation:AnimalsCells
    18. Arvanitis D, Dumas M, Szuchet S. Myelin palingenesis. 2. Immunocytochemical localization of myelin/oligodendrocyte glycolipids in multilamellar structures. Dev Neurosci. 1992; 14(5-6):328-35. PMID: 1306158.
      Citations:    Fields:    Translation:AnimalsCells
    19. Arvanitis D, Polak PE, Szuchet S. Myelin palingenesis. 1. Electron microscopical localization of myelin/oligodendrocyte proteins in multilamellar structures by the immunogold method. Dev Neurosci. 1992; 14(5-6):313-27. PMID: 1284852.
      Citations: 1     Fields:    Translation:AnimalsCells
    20. Soliven B, Szuchet S, Nelson DJ. Tumor necrosis factor inhibits K+ current expression in cultured oligodendrocytes. J Membr Biol. 1991 Nov; 124(2):127-37. PMID: 1662283.
      Citations: 11     Fields:    Translation:HumansAnimalsCells
    21. Szuchet S, Yim SH. Adhesion-induced differentiation of oligodendrocytes signals the synthesis and polarization of heparan sulfate proteoglycans and sulfated glycoproteins. Ann N Y Acad Sci. 1991; 633:530-4. PMID: 1789575.
      Citations:    Fields:    Translation:Cells
    22. Hertz L, Soliven B, Hertz E, Szuchet S, Nelson DJ. Channel-mediated and carrier-mediated uptake of K+ into cultured ovine oligodendrocytes. Glia. 1990; 3(6):550-7. PMID: 2148557.
      Citations: 4     Fields:    Translation:AnimalsCells
    23. Vartanian T, Dawson G, Soliven B, Nelson DJ, Szuchet S. Phosphorylation of myelin basic protein in intact oligodendrocytes: inhibition by galactosylsphingosine and cyclic AMP. Glia. 1989; 2(5):370-9. PMID: 2478466.
      Citations: 9     Fields:    Translation:AnimalsCells
    24. Soliven B, Szuchet S, Arnason BG, Nelson DJ. Expression and modulation of K+ currents in oligodendrocytes: possible role in myelinogenesis. Dev Neurosci. 1989; 11(2):118-31. PMID: 2663420.
      Citations: 5     Fields:    Translation:AnimalsCells
    25. Soliven B, Szuchet S, Arnason BG, Nelson DJ. Forskolin and phorbol esters decrease the same K+ conductance in cultured oligodendrocytes. J Membr Biol. 1988 Oct; 105(2):177-86. PMID: 3216367.
      Citations: 11     Fields:    Translation:AnimalsCells
    26. Soliven B, Szuchet S, Arnason BG, Nelson DJ. Voltage-gated potassium currents in cultured ovine oligodendrocytes. J Neurosci. 1988 Jun; 8(6):2131-41. PMID: 2838593; PMCID: PMC6569331.
      Citations: 12     Fields:    Translation:AnimalsCells
    27. Vartanian T, Sprinkle TJ, Dawson G, Szuchet S. Oligodendrocyte substratum adhesion modulates expression of adenylate cyclase-linked receptors. Proc Natl Acad Sci U S A. 1988 Feb; 85(3):939-43. PMID: 2448785; PMCID: PMC279672.
      Citations: 10     Fields:    Translation:AnimalsCells
    28. Szuchet S, Polak PE, Yim SH, Arvanitis D. Plasma membrane of cultured oligodendrocytes: III. Relatedness to myelin. Glia. 1988; 1(2):141-50. PMID: 2465269.
      Citations: 2     Fields:    Translation:AnimalsCells
    29. Vartanian T, Dawson G, Szuchet S. Intracellular messengers. Influence of oligodendrocyte substratum adhesion. Ann N Y Acad Sci. 1988; 540:433-6. PMID: 2462818.
      Citations:    Fields:    Translation:AnimalsCells
    30. Polak PE, Szuchet S. Plasma membrane of cultured oligodendrocytes: I. Isolation, purification, and initial characterization. Glia. 1988; 1(1):39-53. PMID: 2976738.
      Citations:    Fields:    Translation:AnimalsCells
    31. Mikol DD, Szuchet S, Stefansson K. A peanut agglutinin binding glycoprotein in CNS myelin and oligodendrocytes. Ann N Y Acad Sci. 1988; 540:409-12. PMID: 3207269.
      Citations: 1     Fields:    Translation:HumansAnimalsCells
    32. Szuchet S, Polak PE, Yim SH, Lange Y. Plasma membrane of cultured oligodendrocytes: II. Possible structural and functional domains. Glia. 1988; 1(1):54-63. PMID: 2976739.
      Citations:    Fields:    Translation:AnimalsCells
    33. Vartanian T, Szuchet S, Dawson G, Campagnoni AT. Oligodendrocyte adhesion activates protein kinase C-mediated phosphorylation of myelin basic protein. Science. 1986 Dec 12; 234(4782):1395-8. PMID: 2431483.
      Citations: 22     Fields:    Translation:HumansCells
    34. Yim SH, Szuchet S, Polak PE. Cultured oligodendrocytes. A role for cell-substratum interaction in phenotypic expression. J Biol Chem. 1986 Sep 05; 261(25):11808-15. PMID: 3745167.
      Citations: 10     Fields:    Translation:AnimalsCells
    35. Yim SH, Monsma S, Hertz L, Szuchet S. Lipid and glycolipid metabolism of cultured astrocytes: a time course study. J Neurosci Res. 1986; 15(1):29-37. PMID: 3007768.
      Citations: 2     Fields:    Translation:AnimalsCells
    36. Szuchet S, Polak PE, Yim SH. Mature oligodendrocytes cultured in the absence of neurons recapitulate the ontogenic development of myelin membranes. Dev Neurosci. 1986; 8(4):208-21. PMID: 2435511.
      Citations: 13     Fields:    Translation:AnimalsCells
    37. Dumas M, Zurbriggen A, Vandevelde M, Yim SH, Arnason BG, Szuchet S, Meier C. A monoclonal antibody that binds to both astrocytes and myelin sheaths. J Neuroimmunol. 1985 Jul; 9(1-2):55-67. PMID: 4008637.
      Citations: 5     Fields:    Translation:AnimalsCells
    38. Stowring L, Haase AT, Petursson G, Georgsson G, Palsson P, Lutley R, Roos R, Szuchet S. Detection of visna virus antigens and RNA in glial cells in foci of demyelination. Virology. 1985 Mar; 141(2):311-8. PMID: 3002020.
      Citations: 19     Fields:    Translation:AnimalsCells
    39. Massa PT, Szuchet S, Mugnaini E. Cell-cell interactions of isolated and cultured oligodendrocytes: formation of linear occluding junctions and expression of peculiar intramembrane particles. J Neurosci. 1984 Dec; 4(12):3128-39. PMID: 6502227; PMCID: PMC6564850.
      Citations: 6     Fields:    Translation:AnimalsCells
    40. Szuchet S, Yim SH. Characterization of a subset of oligodendrocytes separated on the basis of selective adherence properties. J Neurosci Res. 1984; 11(2):131-44. PMID: 6200605.
      Citations: 6     Fields:    Translation:AnimalsCells
    41. Szuchet S, Yim SH, Monsma S. Lipid metabolism of isolated oligodendrocytes maintained in long-term culture mimics events associated with myelinogenesis. Proc Natl Acad Sci U S A. 1983 Nov; 80(22):7019-23. PMID: 6580624; PMCID: PMC390118.
      Citations: 8     Fields:    Translation:AnimalsCells
    42. Szuchet S, Dumas M. An in-vitro approach to the study of oligodendrocytes and their involvement in multiple sclerosis. Neurol Clin. 1983 Aug; 1(3):729-55. PMID: 6680171.
      Citations: 1     Fields:    Translation:HumansCells
    43. Szuchet S, Polak PE. A simple cell disrupter designed for small cells with relatively large nuclei. Anal Biochem. 1983 Feb 01; 128(2):453-8. PMID: 6846820.
      Citations: 1     Fields:    Translation:AnimalsCells
    44. Oger J, Szuchet S, Antel J, Arnason BG. A monoclonal antibody against human T suppressor lymphocytes binds specifically to the surface of cultured oligodendrocytes. Nature. 1982 Jan 07; 295(5844):66-8. PMID: 6173754.
      Citations: 16     Fields:    Translation:AnimalsCells
    45. Mack SR, Szuchet S. Synthesis of myelin glycosphingolipids by isolated oligodendrocytes in tissue culture. Brain Res. 1981 Jun 09; 214(1):180-5. PMID: 7237158.
      Citations: 3     Fields:    Translation:AnimalsCells
    46. Mack SR, Szuchet S, Dawson G. Synthesis of gangliosides by cultured oligodendrocytes. J Neurosci Res. 1981; 6(3):361-7. PMID: 7299846.
      Citations: 2     Fields:    Translation:AnimalsCells
    47. Wollmann RL, Szuchet S, Barlow J, Jerkovic M. Ultrastructural changes accompanying the growth of isolated oligodendrocytes. J Neurosci Res. 1981; 6(6):757-69. PMID: 7334534.
      Citations: 2     Fields:    Translation:AnimalsCells
    48. Szuchet S, Stefansson K, Wollmann RL, Dawson G, Arnason BG. Maintenance of isolated oligodendrocytes in long-term culture. Brain Res. 1980 Oct 27; 200(1):151-64. PMID: 6251943.
      Citations: 9     Fields:    Translation:AnimalsCells
    49. Szuchet S, Arnason BG, Polak PE. Separation of ovine oligodendrocytes into two distinct bands on a linear sucrose gradient. J Neurosci Methods. 1980 Oct; 3(1):7-19. PMID: 7230879.
      Citations: 6     Fields:    Translation:Animals
    50. Szuchet S. Effect of purification procedures on the self-association of myosin at high ionic strength. Arch Biochem Biophys. 1977 Apr 30; 180(2):493-503. PMID: 879796.
      Citations: 1     Fields:    Translation:AnimalsCells
    51. Szuchet S. Equilibrium centrifugation of proteins in acid solutions. Ovalbumin in aqueous acetic, propionic, and butyric acids. Arch Biochem Biophys. 1976 Dec; 177(2):437-60. PMID: 1015828.
      Citations: 1     Fields:    
    52. Szuchet S, Yphantis DA. Equilibrium centrifugation of proteins in acidic solutions. Beta-lactoglobulin A in aqueous acetic, propionic, and butyric acids. Arch Biochem Biophys. 1976 Apr; 173(2):495-516. PMID: 1275504.
      Citations: 1     Fields:    Translation:Cells
    53. Weiner RE, Szuchet S. The molecular weight of bovine lactoferrin. Biochim Biophys Acta. 1975 May 30; 393(1):143-7. PMID: 237563.
      Citations: 4     Fields:    Translation:Animals
    54. Szuchet S, Zobel CR. The dissociation of myosin in acid. Biochemistry. 1974 Mar 26; 13(7):1482-91. PMID: 4819761.
      Citations:    Fields:    Translation:AnimalsCells
    55. Szuchet S, Yphantis DA. Equilibrium sedimentation of proteins in acid solutions. Dissociation of aldolase by aqueous acetic acid. Biochemistry. 1973 Dec 04; 12(25):5115-27. PMID: 4799941.
      Citations:    Fields:    Translation:Animals
    56. DERECHIN M, JOHNSON P, SZUCHET S. Further studies with human plasminogen. Biochem J. 1962 Aug; 84:336-9. PMID: 13885558; PMCID: PMC1243671.
      Citations: 1     Fields:    
    57. DERECHIN M, SZUCHET S. Gelation phenomena in degraded fibrinogen and fibrin. Arch Biochem Biophys. 1960 Mar; 87:100-3. PMID: 13816017.
      Citations: 1     Fields:    
    58. SZUCHET S, DERECHIN M. Relation between degree of fibrinogen proteolysis by plasmin and fibrinolytic paracoagulation. Proc Soc Exp Biol Med. 1957 Feb; 94(2):267-9. PMID: 13408230.
      Citations: 1     Fields:    Translation:Cells
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