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Sally Horne-Badovinac

TitleProfessor
InstitutionUniversity of Chicago
DepartmentMolecular Genetics and Cell Biology
AddressChicago IL 60637
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    Collapse Overview 
    Collapse overview
    My lab takes an integrative approach to studying organ morphogenesis. We are currently probing how cells migrate collectively & how basement membrane ECMs are assembled.

    Collective cell migration - The collective migration of cells within an epithelial sheet underlies tissue remodeling events associated with morphogenesis, wound repair, and the spread of many cancers. Yet little is known about how each epithelial cell coordinates its individual migratory behaviors with those of its neighbors, or how epithelial motility shapes tissues during development. We recently discovered a novel planar signaling system that coordinates leading and trailing edge dynamics between neighboring epithelial cells, and are now working to elucidate the mechanistic basis of this signaling. We are also investigating how collective cell migration can be harnesses to create a stable, planar tissue pattern within an epithelium for subsequent morphogenesis.

    Basement membrane dynamics – Basement membrane is a sheet-like extracellular matrix that lines the basal surface of all epithelial tissues. This complex protein network provides structural stability to the monolayer, promotes cell-cell and cell-matrix signaling, and acts as a physical barrier to metastasis. Despite their ubiquity and many essential functions, we know surprisingly little about how basement membranes are built or how they are remodeled during development. We are taking a comprehensive approach to identifying: the cellular mechanisms underlying basement membrane biosynthesis and secretion, how basement membrane biosynthetic programs are modulated to construct diverse matrix architectures, and how different basement membrane architectures ultimately influence organ morphogenesis.

    Experimental system – We perform these studies in the Drosophila egg chamber, which is an organ-like structure in the ovary that will give rise to one egg. Each egg chamber is comprised of a germ cell cluster, surrounded by a somatic epithelium of follicle cells. The follicle cells produce their own basement membrane, which ensheaths the organ. Though initially spherical, egg chambers lengthen as they mature. This morphogenesis depends on a dramatic collective migration of the follicle cells across their basement membrane, which causes the entire egg chamber to rotate within its surrounding matrix. Importantly, the follicle cells also remodel the basement membrane as they crawl along its surface, creating a polarized array of fibrils in the direction of tissue movement. This fibrillar matrix is then thought to act as a “molecular corset” that resists the expansive growth of the germ cells to directionally bias egg chamber growth, and thus create the elongated shape of the egg. A major strength of this system is that the collective migration and basement membrane remodeling both occur on the egg chamber’s outer surface, which allows us to image dynamic cell behaviors - in a living organ - with exquisite clarity. Moreover, we can use the sophisticated genetic tools of Drosophila to identify the underlying molecular mechanisms. Please check out the papers below to see what we have already discovered!

    Collapse Biography 
    Collapse education and training
    UC BerkeleyPostdoc2008Cell and Developmental Biology
    UC San FranciscoPhD2003Biochemistry
    University of OregonBS1996Chemistry
    Collapse awards and honors
    2021Quantrell Award for Excellence in Undergraduate Teaching, The University of Chicago
    2010 - 2012Basil O'Connor Starter Scholar Research Award, The March of Dimes Foundation
    2009 - 2012Young Investigator Award , Edward Mallinckrodt, Jr. Foundation
    2003 - 2006Postdoctoral Fellowship, Jane Coffin Childs Foundation
    2003Harold C. Weintraub Graduate Student Award, Fred Hutchinson Cancer Center
    2001 - 2003Predoctoral Fellowship, American Heart Association
    1998 - 2001Predoctoral Fellowship, National Science Foundation
    1996American Foundation of Chemists Award, University of Oregon
    1996Graduated summa cum laude and with Departmental Honors, University of Oregon

    Collapse Research 
    Collapse research activities and funding
    R35GM148285     (HORNE-BADOVINAC, SALLY)Mar 1, 2023 - Jan 31, 2028
    NIH
    Mechanisms of epithelial migration and basement membrane assembly
    Role: Principal Investigator

    R13HD103289     (HORNE-BADOVINAC, SALLY)Jul 29, 2020 - Jun 30, 2021
    NIH
    The 2020 Santa Cruz Developmental Biology Meeting
    Role: Principal Investigator

    R01GM136961     (HORNE-BADOVINAC, SALLY)Apr 1, 2020 - Feb 29, 2024
    NIH
    Mechanisms of basement membrane secretion and assembly
    Role: Principal Investigator

    R01GM126047     (HORNE-BADOVINAC, SALLY)Feb 1, 2018 - Dec 31, 2021
    NIH
    Mechanisms that Coordinate Cellular Movements for Epithelial Migration
    Role: Principal Investigator

    R01GM094276     (HORNE-BADOVINAC, SALLY)Jul 1, 2010 - Jun 30, 2016
    NIH
    Genetic Analysis of Epithelial Morphogenesis and Organ Shape
    Role: Principal Investigator

    T32HD055164     (HORNE-BADOVINAC, SALLY ;PRINCE, VICTORIA E.)Jun 1, 2007 - Apr 30, 2024
    NIH
    Training Program in Developmental Biology
    Role: Co-Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Scepanovic G, Fernandez-Gonzalez R, Tokamov SA, Buiter S, Ullyot A, Williams AM, Horne-Badovinac S, Fehon RG. Cortical tension promotes Kibra degradation via Par-1. Mol Biol Cell. 2024 Jan 01; 35(1):ar2. PMID: 37903240; PMCID: PMC10881160.
      Citations:    Fields:    Translation:AnimalsCells
    2. Williams AM, Horne-Badovinac S. Fat2 polarizes Lar and Sema5c to coordinate the motility of collectively migrating epithelial cells. J Cell Sci. 2024 03 01; 137(5). PMID: 37593878; PMCID: PMC10508692.
      Citations: 1     Fields:    Translation:AnimalsCells
    3. Williams AM, Horne-Badovinac S. Fat2 polarizes Lar and Sema5c to coordinate the motility of collectively migrating epithelial cells. bioRxiv. 2023 Mar 01. PMID: 36909523; PMCID: PMC10002635.
      Citations:    
    4. Zajac AL, Williams AM, Horne-Badovinac S. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers During Drug Treatments. Methods Mol Biol. 2023; 2626:277-289. PMID: 36715910.
      Citations: 1     Fields:    Translation:Animals
    5. Anderson MT, Sherrard K, Horne-Badovinac S. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells. Methods Mol Biol. 2023; 2626:179-191. PMID: 36715905.
      Citations: 1     Fields:    Translation:AnimalsCells
    6. Williams AM, Donoughe S, Munro E, Horne-Badovinac S. Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration. Elife. 2022 09 26; 11. PMID: 36154691; PMCID: PMC9576270.
      Citations: 7     Fields:    Translation:Animals
    7. Zajac AL, Horne-Badovinac S. Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells. Curr Biol. 2022 02 28; 32(4):735-748.e10. PMID: 35021047; PMCID: PMC8891071.
      Citations: 16     Fields:    Translation:AnimalsCells
    8. Sherrard KM, Cetera M, Horne-Badovinac S. DAAM mediates the assembly of long-lived, treadmilling stress fibers in collectively migrating epithelial cells in Drosophila. Elife. 2021 11 23; 10. PMID: 34812144; PMCID: PMC8610420.
      Citations: 7     Fields:    Translation:AnimalsCells
    9. Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BS. ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Mol Biol Cell. 2020 12 15; 31(26):2892-2903. PMID: 33112725; PMCID: PMC7927198.
      Citations: 6     Fields:    Translation:HumansAnimalsCells
    10. Horne-Badovinac S. The Drosophila micropyle as a system to study how epithelia build complex extracellular structures. Philos Trans R Soc Lond B Biol Sci. 2020 10 12; 375(1809):20190561. PMID: 32829690; PMCID: PMC7482212.
      Citations: 7     Fields:    Translation:AnimalsCells
    11. Horne-Badovinac S. Mobilizing the Matrix for Organ Morphogenesis. Dev Cell. 2020 07 06; 54(1):1-2. PMID: 32634396.
      Citations: 2     Fields:    
    12. Williams AM, Horne-Badovinac S. Looking deeper into tissue elongation. Nat Rev Mol Cell Biol. 2020 06; 21(6):305. PMID: 32071437.
      Citations:    Fields:    Translation:AnimalsCells
    13. Isabella A, Horne-Badovinac S, Cerqueira Campos F, Dennis C, Alégot H, Fritsch C, Pouchin P, Bardot O, Mirouse V. Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation. Development. 2020 04 08; 147(7). PMID: 32156755; PMCID: PMC7157587.
      Citations: 16     Fields:    Translation:AnimalsCells
    14. Cerqueira Campos F, Dennis C, Alégot H, Fritsch C, Isabella A, Pouchin P, Bardot O, Horne-Badovinac S, Mirouse V. Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation. Development. 2020 Jan 01. PMID: 34004855.
      Citations:    
    15. Davis MN, Horne-Badovinac S, Naba A. In-silico definition of the Drosophila melanogaster matrisome. Matrix Biol Plus. 2019 Nov; 4:100015. PMID: 33543012; PMCID: PMC7852309.
      Citations: 18     
    16. Dent LG, Manning SA, Kroeger B, Williams AM, Saiful Hilmi AJ, Crea L, Kondo S, Horne-Badovinac S, Harvey KF. The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development. PLoS Genet. 2019 05; 15(5):e1008083. PMID: 31116733; PMCID: PMC6555532.
      Citations: 4     Fields:    Translation:AnimalsCells
    17. Stedden CG, Menegas W, Zajac AL, Williams AM, Cheng S, Horne-Badovinac S, Özkan E. Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila. Curr Biol. 2019 03 18; 29(6):908-920.e6. PMID: 30827914; PMCID: PMC6424623.
      Citations: 16     Fields:    Translation:AnimalsCells
    18. Zajac AL, Horne-Badovinac S. Tissue Structure: A CIVICs Lesson for Adipocytes. Curr Biol. 2017 09 25; 27(18):R1013-R1015. PMID: 28950083.
      Citations: 1     Fields:    Translation:AnimalsCells
    19. Horne-Badovinac S. Fat-like cadherins in cell migration-leading from both the front and the back. Curr Opin Cell Biol. 2017 10; 48:26-32. PMID: 28551508; PMCID: PMC5591768.
      Citations: 4     Fields:    Translation:Animals
    20. Barlan K, Cetera M, Horne-Badovinac S. Fat2 and Lar Define a Basally Localized Planar Signaling System Controlling Collective Cell Migration. Dev Cell. 2017 03 13; 40(5):467-477.e5. PMID: 28292425; PMCID: PMC5354100.
      Citations: 44     Fields:    Translation:AnimalsCells
    21. Williams AM, Horne-Badovinac S. Shaping the Drosophila egg. Mol Reprod Dev. 2016 Dec; 83(12):1045. PMID: 27977054.
      Citations:    
    22. Isabella AJ, Horne-Badovinac S. Rab10-Mediated Secretion Synergizes with Tissue Movement to Build a Polarized Basement Membrane Architecture for Organ Morphogenesis. Dev Cell. 2016 07 11; 38(1):47-60. PMID: 27404358; PMCID: PMC4942852.
      Citations: 50     Fields:    Translation:AnimalsCells
    23. Andersen D, Horne-Badovinac S. Influence of ovarian muscle contraction and oocyte growth on egg chamber elongation in Drosophila. Development. 2016 Apr 15; 143(8):1375-87. PMID: 26952985; PMCID: PMC4852517.
      Citations: 17     Fields:    Translation:AnimalsCells
    24. Cetera M, Lewellyn L, Horne-Badovinac S. Cultivation and Live Imaging of Drosophila Ovaries. Methods Mol Biol. 2016; 1478:215-226. PMID: 27730584.
      Citations: 14     Fields:    Translation:AnimalsCells
    25. Isabella AJ, Horne-Badovinac S. Dynamic regulation of basement membrane protein levels promotes egg chamber elongation in Drosophila. Dev Biol. 2015 Oct 15; 406(2):212-21. PMID: 26348027; PMCID: PMC4639450.
      Citations: 33     Fields:    Translation:Animals
    26. Isabella AJ, Horne-Badovinac S. Building from the Ground up: Basement Membranes in Drosophila Development. Curr Top Membr. 2015; 76:305-36. PMID: 26610918; PMCID: PMC4772872.
      Citations: 17     Fields:    Translation:HumansAnimalsCells
    27. Horne-Badovinac S, Powell K. Sally Horne-Badovinac: Taking a spin around morphogenesis. J Cell Biol. 2015 Jul 06; 210(1):4-5. PMID: 26150385; PMCID: PMC4494012.
      Citations:    Fields:    Translation:HumansAnimals
    28. Cetera M, Horne-Badovinac S. Round and round gets you somewhere: collective cell migration and planar polarity in elongating Drosophila egg chambers. Curr Opin Genet Dev. 2015 Jun; 32:10-5. PMID: 25677931; PMCID: PMC4470743.
      Citations: 30     Fields:    Translation:AnimalsCells
    29. Cetera M, Ramirez-San Juan GR, Oakes PW, Lewellyn L, Fairchild MJ, Tanentzapf G, Gardel ML, Horne-Badovinac S. Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation. Nat Commun. 2014 Nov 21; 5:5511. PMID: 25413675; PMCID: PMC4241503.
      Citations: 100     Fields:    Translation:AnimalsCells
    30. Horne-Badovinac S. The Drosophila egg chamber-a new spin on how tissues elongate. Integr Comp Biol. 2014 Oct; 54(4):667-76. PMID: 24920751; PMCID: PMC4229889.
      Citations: 27     Fields:    Translation:AnimalsCells
    31. Horne-Badovinac S. Cell-cell and cell-matrix interactions. Mol Biol Cell. 2014 Mar; 25(6):731. PMID: 24626845; PMCID: PMC3952839.
      Citations: 1     Fields:    Translation:HumansAnimalsCells
    32. Lewellyn L, Cetera M, Horne-Badovinac S. Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila. J Cell Biol. 2013 Mar 18; 200(6):721-9. PMID: 23509067; PMCID: PMC3601364.
      Citations: 38     Fields:    Translation:AnimalsCells
    33. Lerner DW, McCoy D, Isabella AJ, Mahowald AP, Gerlach GF, Chaudhry TA, Horne-Badovinac S. A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis. Dev Cell. 2013 Jan 28; 24(2):159-68. PMID: 23369713; PMCID: PMC3562474.
      Citations: 76     Fields:    Translation:AnimalsCells
    34. Horne-Badovinac S, Hill J, Gerlach G, Menegas W, Bilder D. A screen for round egg mutants in Drosophila identifies tricornered, furry, and misshapen as regulators of egg chamber elongation. G3 (Bethesda). 2012 Mar; 2(3):371-8. PMID: 22413091; PMCID: PMC3291507.
      Citations: 29     Fields:    
    35. Horne-Badovinac S, Munro E. Developmental biology. Tubular transformations. Science. 2011 Jul 15; 333(6040):294-5. PMID: 21764735.
      Citations:    Fields:    Translation:AnimalsCells
    36. Horne-Badovinac S, Bilder D. Dynein regulates epithelial polarity and the apical localization of stardust A mRNA. PLoS Genet. 2008 Jan; 4(1):e8. PMID: 18208331; PMCID: PMC2213700.
      Citations: 49     Fields:    Translation:AnimalsCells
    37. Horne-Badovinac S, Bilder D. Mass transit: epithelial morphogenesis in the Drosophila egg chamber. Dev Dyn. 2005 Mar; 232(3):559-74. PMID: 15704134.
      Citations: 129     Fields:    Translation:AnimalsCells
    38. Horne-Badovinac S, Rebagliati M, Stainier DY. A cellular framework for gut-looping morphogenesis in zebrafish. Science. 2003 Oct 24; 302(5645):662-5. PMID: 14576439.
      Citations: 43     Fields:    Translation:Animals
    39. Horne-Badovinac S, Lin D, Waldron S, Schwarz M, Mbamalu G, Pawson T, Jan Y, Stainier DY, Abdelilah-Seyfried S, Horne-Badovinac S, Lin D, Waldron S, Schwarz M, Mbamalu G, Pawson T, Jan Y, Stainier DY, Abdelilah-Seyfried S. Positional cloning of heart and soul reveals multiple roles for PKC lambda in zebrafish organogenesis. Curr Biol. 2001 Oct 02; 11(19):1492-502. PMID: 11591316.
      Citations: 103     Fields:    Translation:HumansAnimalsCells
    40. Yelon D, Horne SA, Stainier DY. Restricted expression of cardiac myosin genes reveals regulated aspects of heart tube assembly in zebrafish. Dev Biol. 1999 Oct 01; 214(1):23-37. PMID: 10491254.
      Citations: 209     Fields:    Translation:Animals
    41. Postlethwait JH, Yan YL, Gates MA, Horne S, Amores A, Brownlie A, Donovan A, Egan ES, Force A, Gong Z, Goutel C, Fritz A, Kelsh R, Knapik E, Liao E, Paw B, Ransom D, Singer A, Thomson M, Abduljabbar TS, Yelick P, Beier D, Joly JS, Larhammar D, Rosa F, Westerfield M, Zon LI, Johnson SL, Talbot WS. Vertebrate genome evolution and the zebrafish gene map. Nat Genet. 1998 Apr; 18(4):345-9. PMID: 9537416.
      Citations: 234     Fields:    Translation:Animals
    42. Johnson SL, Gates MA, Johnson M, Talbot WS, Horne S, Baik K, Rude S, Wong JR, Postlethwait JH. Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics. 1996 Apr; 142(4):1277-88. PMID: 8846904; PMCID: PMC1207124.
      Citations: 43     Fields:    Translation:AnimalsCells
    43. Johnson SL, Africa D, Horne S, Postlethwait JH. Half-tetrad analysis in zebrafish: mapping the ros mutation and the centromere of linkage group I. Genetics. 1995 Apr; 139(4):1727-35. PMID: 7789773; PMCID: PMC1206498.
      Citations: 38     Fields:    Translation:AnimalsCells
    44. Postlethwait JH, Johnson SL, Midson CN, Talbot WS, Gates M, Ballinger EW, Africa D, Andrews R, Carl T, Eisen JS, et al. A genetic linkage map for the zebrafish. Science. 1994 Apr 29; 264(5159):699-703. PMID: 8171321.
      Citations: 82     Fields:    Translation:AnimalsCells
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