Michael A. Glotzer
Title | Professor |
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Institution | University of Chicago |
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Department | Molecular Genetics and Cell Biology |
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Address | Chicago IL 60637
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Overview The Glotzer lab focuses on questions related to cell organization. How do cells coordinate the position of the contractile ring with the position of the spindle? By what mechanisms are cortical domains that mediate cell polarization assembled and maintained? How do cells regulate cortical contractility during developmental morphogenesis?
To answer these questions, we use the nematode C. elegans, cultured human cells, budding yeast, and Drosophila as model systems and we combine forward and reverse genetics, biochemistry, and live cell imaging. Through these approaches we have discovered and extensively characterized the centralspindlin complex, a multifunctional protein complex that regulates essentially every step of cytokinesis.
We have pioneered the use of optogenetics to dissect spatiotemporally regulated processes. Using these approaches, we have demonstrated that RhoA activation is sufficient to induce cleavage furrows irrespective of the position of the spindle or the stage of the cell cycle. Likewise, we obtained direct evidence that positive feedback is active during yeast cell polarization.
By combining optogenetics with "conventional" genetics and live cell imaging we can directly test models for how cells endow well defined cortical regions with distinct properties that are required for complex cellular events, like cell division, cell polarization, and tissue scale morphogenetic events, such as gastrulation.
Biography European Molecular Biology Labs, Heidelberg, Germany | Postdoc | 09/1997 | | University of California, San Francisco, San Francisco, CA | Ph.D | 09/1993 | Cell Biology | University of California, Berkeley, Berkeley, CA | A.B. | 05/1985 | Mathematics |
2003 | EMBO Member | 2004 | Keith Porter Fellow, Keith Porter Endowment |
ORNG Applications Research R01GM074743 (GLOTZER, MICHAEL A.)Jul 1, 2006 - Jun 30, 2011 NIH Molecular Dissection of Cytokinesis Role: Principal Investigator |
| R01GM085087 (GLOTZER, MICHAEL A.)Sep 1, 2008 - May 31, 2021 NIH Positioning the Plane of Cell Division During Cytokinesis Role: Principal Investigator |
| R01GM088668 (GLOTZER, MICHAEL A.)Aug 1, 2009 - Jul 31, 2014 NIH Spatial and temporal control of cell behavior with a genetically-encoded photoswi Role: Principal Investigator |
| R35GM127091 (GLOTZER, MICHAEL A.)Aug 1, 2018 - Jul 31, 2023 NIH Spatial and temporal control of Rho family GTPases Role: Principal Investigator |
Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications.
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Rich A, Glotzer M. Small GTPases modulate intrinsic and extrinsic forces that control epithelial folding in Drosophila embryos. Small GTPases. 2021 Sep-Nov; 12(5-6):416-428. PMID: 33985411.
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Wang X, Zhang D, Zheng C, Wu S, Glotzer M, Tse YC. Cortical recruitment of centralspindlin and RhoA effectors during meiosis I of Caenorhabditiselegans primary spermatocytes. J Cell Sci. 2021 02 08; 134(3). PMID: 33468621.
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Rich A, Fehon RG, Glotzer M. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife. 2020 11 17; 9. PMID: 33200987.
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Lu W, Lakonishok M, Liu R, Billington N, Rich A, Glotzer M, Sellers JR, Gelfand VI. Competition between kinesin-1 and myosin-V defines Drosophila posterior determination. Elife. 2020 02 14; 9. PMID: 32057294.
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Adriaans IE, Basant A, Ponsioen B, Glotzer M, Lens SMA. PLK1 plays dual roles in centralspindlin regulation during cytokinesis. J Cell Biol. 2019 04 01; 218(4):1250-1264. PMID: 30728176.
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Basant A, Glotzer M. Spatiotemporal Regulation of RhoA during Cytokinesis. Curr Biol. 2018 05 07; 28(9):R570-R580. PMID: 29738735.
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Glotzer M. Cytokinesis in Metazoa and Fungi. Cold Spring Harb Perspect Biol. 2017 Oct 03; 9(10). PMID: 28007751.
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Basant A, Glotzer M. A GAP that Divides. F1000Res. 2017; 6:1788. PMID: 29043078.
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Witte K, Strickland D, Glotzer M. Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization. Elife. 2017 07 06; 6. PMID: 28682236.
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Oakes PW, Wagner E, Brand CA, Probst D, Linke M, Schwarz US, Glotzer M, Gardel ML. Optogenetic control of RhoA reveals zyxin-mediated elasticity of stress fibres. Nat Commun. 2017 06 12; 8:15817. PMID: 28604737.
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Afshar K, Werner ME, Tse YC, Glotzer M, Gönczy P. Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos. Development. 2016 07 15; 143(14):2689. PMID: 27436042.
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Wagner E, Glotzer M. Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage. J Cell Biol. 2016 06 20; 213(6):641-9. PMID: 27298323.
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Zhang D, Glotzer M. Cytokinesis: Placing the Furrow in Context. Curr Biol. 2015 Dec 21; 25(24):R1183-5. PMID: 26702657.
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Spiltoir JI, Strickland D, Glotzer M, Tucker CL. Optical Control of Peroxisomal Trafficking. ACS Synth Biol. 2016 07 15; 5(7):554-60. PMID: 26513473.
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Zhang D, Glotzer M. The RhoGAP activity of CYK-4/MgcRacGAP functions non-canonically by promoting RhoA activation during cytokinesis. Elife. 2015 Aug 07; 4. PMID: 26252513.
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Basant A, Lekomtsev S, Tse YC, Zhang D, Longhini KM, Petronczki M, Glotzer M. Aurora B kinase promotes cytokinesis by inducing centralspindlin oligomers that associate with the plasma membrane. Dev Cell. 2015 Apr 20; 33(2):204-15. PMID: 25898168.
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White EA, Raghuraman H, Perozo E, Glotzer M. Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit. J Biol Chem. 2013 Jul 05; 288(27):19785-95. PMID: 23720745.
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Glotzer M. Cytokinesis: centralspindlin moonlights as a membrane anchor. Curr Biol. 2013 Feb 18; 23(4):R145-7. PMID: 23428321.
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White EA, Glotzer M. Centralspindlin: at the heart of cytokinesis. Cytoskeleton (Hoboken). 2012 Nov; 69(11):882-92. PMID: 22927365.
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Tse YC, Werner M, Longhini KM, Labbe JC, Goldstein B, Glotzer M. RhoA activation during polarization and cytokinesis of the early Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-4. Mol Biol Cell. 2012 Oct; 23(20):4020-31. PMID: 22918944.
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Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. TULIPs: tunable, light-controlled interacting protein tags for cell biology. Nat Methods. 2012 Mar 04; 9(4):379-84. PMID: 22388287.
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Loria A, Longhini KM, Glotzer M. The RhoGAP domain of CYK-4 has an essential role in RhoA activation. Curr Biol. 2012 Feb 07; 22(3):213-9. PMID: 22226748.
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Tse YC, Piekny A, Glotzer M. Anillin promotes astral microtubule-directed cortical myosin polarization. Mol Biol Cell. 2011 Sep; 22(17):3165-75. PMID: 21737681.
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Glotzer M. Cytokinesis: integrating signaling, the cytoskeleton, and membranes to create new daughter cells. Semin Cell Dev Biol. 2010 Dec; 21(9):865. PMID: 20888420.
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Glotzer M. Controlling cytokinesis through promiscuous phosphorylation outside BARs. Mol Cell. 2010 Jul 09; 39(1):3-5. PMID: 20603070.
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Afshar K, Werner ME, Tse YC, Glotzer M, Gönczy P. Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos. Development. 2010 Jan; 137(2):237-47. PMID: 20040490.
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Hutterer A, Glotzer M, Mishima M. Clustering of centralspindlin is essential for its accumulation to the central spindle and the midbody. Curr Biol. 2009 Dec 15; 19(23):2043-9. PMID: 19962307.
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Wolfe BA, Takaki T, Petronczki M, Glotzer M. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. PLoS Biol. 2009 May 05; 7(5):e1000110. PMID: 19468300.
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Wolfe BA, Glotzer M. Single cells (put a ring on it). Genes Dev. 2009 Apr 15; 23(8):896-901. PMID: 19390083.
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Glotzer M. Cytokinesis: GAP gap. Curr Biol. 2009 Feb 24; 19(4):R162-5. PMID: 19243691.
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Glotzer M. The 3Ms of central spindle assembly: microtubules, motors and MAPs. Nat Rev Mol Cell Biol. 2009 Jan; 10(1):9-20. PMID: 19197328.
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Slatkin DN, Blattmann H, Wagner HP, Glotzer MA, Laissue JA. Prospects for microbeam radiation therapy of brain tumours in children. Dev Med Child Neurol. 2009 Feb; 51(2):163. PMID: 19018844.
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Werner M, Glotzer M. Control of cortical contractility during cytokinesis. Biochem Soc Trans. 2008 Jun; 36(Pt 3):371-7. PMID: 18481961.
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Simon GC, Schonteich E, Wu CC, Piekny A, Ekiert D, Yu X, Gould GW, Glotzer M, Prekeris R. Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO J. 2008 Jul 09; 27(13):1791-803. PMID: 18511905.
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Kieserman EK, Glotzer M, Wallingford JB. Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis. Curr Biol. 2008 Jan 22; 18(2):116-23. PMID: 18207743.
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Piekny AJ, Glotzer M. Anillin is a scaffold protein that links RhoA, actin, and myosin during cytokinesis. Curr Biol. 2008 Jan 08; 18(1):30-6. PMID: 18158243.
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Pavicic-Kaltenbrunner V, Mishima M, Glotzer M. Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex. Mol Biol Cell. 2007 Dec; 18(12):4992-5003. PMID: 17942600.
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Werner M, Munro E, Glotzer M. Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis. Curr Biol. 2007 Aug 07; 17(15):1286-97. PMID: 17669650.
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Petronczki M, Glotzer M, Kraut N, Peters JM. Polo-like kinase 1 triggers the initiation of cytokinesis in human cells by promoting recruitment of the RhoGEF Ect2 to the central spindle. Dev Cell. 2007 May; 12(5):713-25. PMID: 17488623.
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Hizlan D, Mishima M, Tittmann P, Gross H, Glotzer M, Hoenger A. Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubules. J Struct Biol. 2006 Jan; 153(1):73-84. PMID: 16361109.
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Piekny A, Werner M, Glotzer M. Cytokinesis: welcome to the Rho zone. Trends Cell Biol. 2005 Dec; 15(12):651-8. PMID: 16243528.
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Yüce O, Piekny A, Glotzer M. An ECT2-centralspindlin complex regulates the localization and function of RhoA. J Cell Biol. 2005 Aug 15; 170(4):571-82. PMID: 16103226.
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Guse A, Mishima M, Glotzer M. Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Curr Biol. 2005 Apr 26; 15(8):778-86. PMID: 15854913.
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Glotzer M. The molecular requirements for cytokinesis. Science. 2005 Mar 18; 307(5716):1735-9. PMID: 15774750.
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Balasubramanian MK, Bi E, Glotzer M. Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells. Curr Biol. 2004 Sep 21; 14(18):R806-18. PMID: 15380095.
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Mishima M, Pavicic V, Grüneberg U, Nigg EA, Glotzer M. Cell cycle regulation of central spindle assembly. Nature. 2004 Aug 19; 430(7002):908-13. PMID: 15282614.
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Glotzer M. Cleavage furrow positioning. J Cell Biol. 2004 Feb 02; 164(3):347-51. PMID: 14757750.
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Glotzer M. Cytokinesis: progress on all fronts. Curr Opin Cell Biol. 2003 Dec; 15(6):684-90. PMID: 14644192.
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Mishima M, Glotzer M. Cytokinesis: a logical GAP. Curr Biol. 2003 Aug 05; 13(15):R589-91. PMID: 12906808.
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Romano A, Guse A, Krascenicova I, Schnabel H, Schnabel R, Glotzer M. CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1. J Cell Biol. 2003 Apr 28; 161(2):229-36. PMID: 12707312.
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Schleiffer A, Kaitna S, Maurer-Stroh S, Glotzer M, Nasmyth K, Eisenhaber F. Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners. Mol Cell. 2003 Mar; 11(3):571-5. PMID: 12667442.
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Dechant R, Glotzer M. Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation. Dev Cell. 2003 Mar; 4(3):333-44. PMID: 12636915.
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Macara IG, Baldarelli R, Field CM, Glotzer M, Hayashi Y, Hsu SC, Kennedy MB, Kinoshita M, Longtine M, Low C, Maltais LJ, McKenzie L, Mitchison TJ, Nishikawa T, Noda M, Petty EM, Peifer M, Pringle JR, Robinson PJ, Roth D, Russell SE, Stuhlmann H, Tanaka M, Tanaka T, Trimble WS, Ware J, Zeleznik-Le NJ, Zieger B. Mammalian septins nomenclature. Mol Biol Cell. 2002 Dec; 13(12):4111-3. PMID: 12475938.
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Mendoza M, Hyman AA, Glotzer M. GTP binding induces filament assembly of a recombinant septin. Curr Biol. 2002 Oct 29; 12(21):1858-63. PMID: 12419187.
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Gruneberg U, Glotzer M, Gartner A, Nigg EA. The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo. J Cell Biol. 2002 Sep 02; 158(5):901-14. PMID: 12213836.
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Kaitna S, Schnabel H, Schnabel R, Hyman AA, Glotzer M. A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo. J Cell Sci. 2002 Jun 01; 115(Pt 11):2293-302. PMID: 12006614.
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Glotzer M, Dechant R. Cytokinesis: regulated by destruction. Curr Biol. 2002 May 14; 12(10):R344-6. PMID: 12015132.
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Kaitna S, Pasierbek P, Jantsch M, Loidl J, Glotzer M. The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis. Curr Biol. 2002 May 14; 12(10):798-812. PMID: 12015116.
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Mishima M, Kaitna S, Glotzer M. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev Cell. 2002 Jan; 2(1):41-54. PMID: 11782313.
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Siomos MF, Badrinath A, Pasierbek P, Livingstone D, White J, Glotzer M, Nasmyth K. Separase is required for chromosome segregation during meiosis I in Caenorhabditis elegans. Curr Biol. 2001 Nov 27; 11(23):1825-35. PMID: 11728305.
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Glotzer M. Animal cell cytokinesis. Annu Rev Cell Dev Biol. 2001; 17:351-86. PMID: 11687493.
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Kaitna S, Mendoza M, Jantsch-Plunger V, Glotzer M. Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis. Curr Biol. 2000 Oct 05; 10(19):1172-81. PMID: 11050385.
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Jantsch-Plunger V, Gönczy P, Romano A, Schnabel H, Hamill D, Schnabel R, Hyman AA, Glotzer M. CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis. J Cell Biol. 2000 Jun 26; 149(7):1391-404. PMID: 10871280.
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Jantsch-Plunger V, Glotzer M. Depletion of syntaxins in the early Caenorhabditis elegans embryo reveals a role for membrane fusion events in cytokinesis. Curr Biol. 1999 Jul 15; 9(14):738-45. PMID: 10421575.
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Glotzer M. Chromosome segregation: Samurai separation of Siamese sisters. Curr Biol. 1999 Jul 15; 9(14):R531-4. PMID: 10421570.
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Glotzer M. The mechanism and control of cytokinesis. Curr Opin Cell Biol. 1997 Dec; 9(6):815-23. PMID: 9425346.
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Wheatley SP, Hinchcliffe EH, Glotzer M, Hyman AA, Sluder G, Wang Yl. CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo. J Cell Biol. 1997 Jul 28; 138(2):385-93. PMID: 9230080.
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Glotzer M. Cytokinesis. Curr Biol. 1997 May 01; 7(5):R274-6. PMID: 9115401.
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Glotzer JB, Saffrich R, Glotzer M, Ephrussi A. Cytoplasmic flows localize injected oskar RNA in Drosophila oocytes. Curr Biol. 1997 May 01; 7(5):326-37. PMID: 9115398.
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Drechsel DN, Hyman AA, Hall A, Glotzer M. A requirement for Rho and Cdc42 during cytokinesis in Xenopus embryos. Curr Biol. 1997 Jan 01; 7(1):12-23. PMID: 8999996.
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Glotzer M. Mitosis: don't get mad, get even. Curr Biol. 1996 Dec 01; 6(12):1592-4. PMID: 8994822.
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King RW, Glotzer M, Kirschner MW. Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates. Mol Biol Cell. 1996 Sep; 7(9):1343-57. PMID: 8885231.
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Glotzer M, Hyman AA. Cell polarity. The importance of being polar. Curr Biol. 1995 Oct 01; 5(10):1102-5. PMID: 8548277.
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Glotzer M. Cell cycle. The only way out of mitosis. Curr Biol. 1995 Sep 01; 5(9):970-2. PMID: 8542285.
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Holloway SL, Glotzer M, King RW, Murray AW. Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor. Cell. 1993 Jul 02; 73(7):1393-402. PMID: 8391932.
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Glotzer M, Murray AW, Kirschner MW. Cyclin is degraded by the ubiquitin pathway. Nature. 1991 Jan 10; 349(6305):132-8. PMID: 1846030.
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Solomon MJ, Glotzer M, Lee TH, Philippe M, Kirschner MW. Cyclin activation of p34cdc2. Cell. 1990 Nov 30; 63(5):1013-24. PMID: 2147872.
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Gautier J, Minshull J, Lohka M, Glotzer M, Hunt T, Maller JL. Cyclin is a component of maturation-promoting factor from Xenopus. Cell. 1990 Feb 09; 60(3):487-94. PMID: 1967981.
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Jacobsson A, Stadler U, Glotzer MA, Kozak LP. Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression. J Biol Chem. 1985 Dec 25; 260(30):16250-4. PMID: 2999153.
This graph shows the total number of publications by year, by first, middle/unknown, or last author.
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Year | Publications |
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1985 | 1 | 1990 | 2 | 1991 | 1 | 1993 | 1 | 1995 | 2 | 1996 | 2 | 1997 | 5 | 1999 | 2 | 2000 | 2 | 2001 | 2 | 2002 | 7 | 2003 | 5 | 2004 | 3 | 2005 | 4 | 2006 | 1 | 2007 | 4 | 2008 | 4 | 2009 | 5 | 2010 | 3 | 2011 | 1 | 2012 | 4 | 2013 | 2 | 2015 | 4 | 2016 | 2 | 2017 | 4 | 2018 | 1 | 2019 | 1 | 2020 | 2 | 2021 | 2 |
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