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Search Results to Heng-Chi Lee

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One or more keywords matched the following properties of Lee, Heng-Chi

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keywords Small Non-Coding RNA
keywords RNA Processing, Post Transcriptional
overview Small non-coding RNAs play remarkable roles in regulating gene expression by binding to Argonaute family proteins and guiding them to recognize their targets. We are interested in understanding how small RNAs function as a surveillance system that defends the genome against foreign nucleic acids, such as transposons. A conserved subfamily of Argonaute, named PIWI, binds PIWI-interacting RNA (piRNA) and plays critical roles in silencing transposons in various animals. Using C. elegans as a model organism, our recent study suggests PIWI and >25,000 genome-encoded piRNAs survey the whole transcriptome to detect "non-self" nucleic acids. Interestingly, our research demonstrated that piRNAs can establish "non-self memory" that silences its targets over multiple generations! In addition, self-transcripts appear to be protected by another group of small RNAs from piRNA silencing. Genetic analyses have laid the groundwork for our knowledge of this defense system, but the molecular mechanisms are still poorly understood. We aim to investigate several fundamental questions including: 1. How do piRNAs recognize their RNA targets/ 2. How are "self" and "non-self" memories established and inherited? 3. How are various small RNAs produced and specifically associated with distinct Argonaute complexes? By applying complementary approaches including genomics, genetics and biochemistry, we aim to identify important principles of small RNA-based genome defense mechanisms that are likely conserved between different animals. Importantly, as PIWI mutants exhibit fertility defects in various animals, we hope our research will provide important insights toward the molecular pathogenesis of infertility.

One or more keywords matched the following items that are connected to Lee, Heng-Chi

Item TypeName
Concept RNA, Fungal
Concept RNA Replicase
Concept RNA
Concept RNA Polymerase II
Concept RNA Processing, Post-Transcriptional
Concept RNA Interference
Concept RNA Caps
Concept RNA, Double-Stranded
Concept RNA, Messenger
Concept DEAD-box RNA Helicases
Concept RNA, Small Interfering
Concept RNA, Helminth
Academic Article The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes.
Academic Article CapSeq and CIP-TAP identify Pol II start sites and reveal capped small RNAs as C. elegans piRNA precursors.
Academic Article Diverse pathways generate microRNA-like RNAs and Dicer-independent small interfering RNAs in fungi.
Academic Article qiRNA is a new type of small interfering RNA induced by DNA damage.
Academic Article The DNA/RNA-dependent RNA polymerase QDE-1 generates aberrant RNA and dsRNA for RNAi in a process requiring replication protein A and a DNA helicase.
Academic Article piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline.
Academic Article C. elegans piRNAs mediate the genome-wide surveillance of germline transcripts.
Academic Article The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans.
Grant Mechanism of piRNA-mediated recognition and silencing of foreign genes
Academic Article piRTarBase: a database of piRNA targeting sites and their roles in gene regulation.
Academic Article pirScan: a webserver to predict piRNA targeting sites and to avoid transgene silencing in C. elegans.
Academic Article The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditis elegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle.
Academic Article The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditis elegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle.

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  • RNA