Two-Hybrid System Techniques
                             
                            
                            
                                
                            
                            
                                
                            
                            
                            
                                
                                    
                                            
	"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, 
	MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, 
	which enables searching at various levels of specificity.
	
	
		
			
			
				Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
    
			
			
				
				
					
						| Descriptor ID | D020798 | 
					
						| MeSH Number(s) | E05.393.220.870 E05.601.690.650 E05.601.870 | 
					
						| Concept/Terms | Two-Hybrid System TechniquesTwo-Hybrid System TechniquesTechnique, Two-Hybrid SystemTechniques, Two-Hybrid SystemTwo Hybrid System TechniquesTwo-Hybrid System TechniqueTwo-Hybrid MethodMethod, Two-HybridMethods, Two-HybridTwo Hybrid MethodTwo-Hybrid MethodsTwo-Hybrid System TechnicsTechnic, Two-Hybrid SystemTechnics, Two-Hybrid SystemTwo Hybrid System TechnicsTwo-Hybrid System TechnicTwo-Hybrid AssayAssay, Two-HybridAssays, Two-HybridTwo Hybrid AssayTwo-Hybrid Assays
 One-Hybrid System TechniquesOne-Hybrid System TechniquesOne Hybrid System TechniquesOne-Hybrid System TechniqueTechnique, One-Hybrid SystemTechniques, One-Hybrid SystemOne-Hybrid System TechnicsOne Hybrid System TechnicsOne-Hybrid System TechnicTechnic, One-Hybrid SystemTechnics, One-Hybrid System
 Yeast Two-Hybrid System TechniquesYeast Two-Hybrid System TechniquesYeast Two Hybrid System TechniquesYeast Two-Hybrid SystemSystem, Yeast Two-HybridSystems, Yeast Two-HybridTwo-Hybrid System, YeastTwo-Hybrid Systems, YeastYeast Two Hybrid SystemYeast Two-Hybrid SystemsYeast Two-Hybrid AssayAssay, Yeast Two-HybridAssays, Yeast Two-HybridTwo-Hybrid Assay, YeastTwo-Hybrid Assays, YeastYeast Two Hybrid AssayYeast Two-Hybrid Assays
 Three-Hybrid System TechniquesThree-Hybrid System TechniquesTechnique, Three-Hybrid SystemTechniques, Three-Hybrid SystemThree Hybrid System TechniquesThree-Hybrid System TechniqueTri-Hybrid System TechniquesTechnique, Tri-Hybrid SystemTechniques, Tri-Hybrid SystemTri Hybrid System TechniquesTri-Hybrid System TechniqueThree-Hybrid System TechnicsTechnic, Three-Hybrid SystemTechnics, Three-Hybrid SystemThree Hybrid System TechnicsThree-Hybrid System Technic
 Yeast Three-Hybrid System TechniquesYeast Three-Hybrid System TechniquesYeast Three Hybrid System TechniquesYeast Three-Hybrid SystemSystem, Yeast Three-HybridSystems, Yeast Three-HybridThree-Hybrid System, YeastThree-Hybrid Systems, YeastYeast Three Hybrid SystemYeast Three-Hybrid SystemsYeast Three-Hybrid AssayAssay, Yeast Three-HybridAssays, Yeast Three-HybridThree-Hybrid Assay, YeastThree-Hybrid Assays, YeastYeast Three Hybrid AssayYeast Three-Hybrid Assays
 n-Hybrid System Techniquesn-Hybrid System TechniquesSystem Technique, n-HybridSystem Techniques, n-HybridTechnique, n-Hybrid SystemTechniques, n-Hybrid Systemn Hybrid System Techniquesn-Hybrid System Technique
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				Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".
				
			 
			
			
				Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".
				
			 
		 
	 
 
                                        
                                            
	
	
		
			
			
					
				This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications. 
				
					 
                    To see the data from this visualization as text, 
click here. 
                
		            | Year | Major Topic | Minor Topic | Total | 
|---|
| 1999 | 0 | 1 | 1 | 
| 2000 | 0 | 3 | 3 | 
| 2001 | 0 | 5 | 5 | 
| 2002 | 0 | 5 | 5 | 
| 2003 | 0 | 4 | 4 | 
| 2004 | 0 | 4 | 4 | 
| 2005 | 0 | 6 | 6 | 
| 2007 | 2 | 1 | 3 | 
| 2008 | 0 | 2 | 2 | 
| 2009 | 0 | 4 | 4 | 
| 2010 | 0 | 2 | 2 | 
| 2011 | 0 | 1 | 1 | 
| 2012 | 0 | 1 | 1 | 
| 2013 | 0 | 3 | 3 | 
| 2014 | 0 | 1 | 1 | 
| 2015 | 0 | 1 | 1 | 
| 2016 | 0 | 1 | 1 | 
| 2017 | 1 | 0 | 1 | 
| 2018 | 0 | 2 | 2 | 
| 2022 | 0 | 1 | 1 | 
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				Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.
						
					
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								The cargo adapter protein CLINT1 is phosphorylated by the Numb-associated kinase BIKE and mediates dengue virus infection. J Biol Chem. 2022 06; 298(6):101956. 
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								Coevolution-based inference of amino acid interactions underlying protein function. Elife. 2018 07 20; 7. 
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								Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat Commun. 2018 03 13; 9(1):1053. 
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								CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. Nat Methods. 2017 08; 14(8):819-825. 
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								The sodium chloride cotransporter (NCC) and epithelial sodium channel (ENaC) associate. Biochem J. 2016 10 01; 473(19):3237-52. 
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								Optical Control of Peroxisomal Trafficking. ACS Synth Biol. 2016 07 15; 5(7):554-60. 
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								Historical contingency and its biophysical basis in glucocorticoid receptor evolution. Nature. 2014 Aug 14; 512(7513):203-7. 
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								Identification and comparative analysis of hepatitis C virus-host cell protein interactions. Mol Biosyst. 2013 Dec; 9(12):3199-209. 
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								Sec16 influences transitional ER sites by regulating rather than organizing COPII. Mol Biol Cell. 2013 Nov; 24(21):3406-19. 
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								Exit from competence for genetic transformation in Streptococcus pneumoniae is regulated at multiple levels. PLoS One. 2013; 8(5):e64197.